Transcriptome analysis reveals underlying immune response mechanism of fungal disease in Gastrodia elata Bl. f. glauca S. Chow

Abstract

Background Gastrodia elata Bl. f. glauca S. Chow is a rare medicinal plant. G. elata f. glauca is unavoidably infected by pathogens in their growth process, for they are usually grown in a semi-wild state. If it happens, that will seriously affect the yield and quality of their tubers. In previous work, we have successfully isolated and identified the pathogenic fungus from infected tubers in G. elata f. glauca. In this study, healthy and fungal diseased mature tubers of G. elata f. glauca from Changbai Mountain area were used as experimental materials. Results A total of 7540 differentially expressed Unigenes (DEGs) were identified (FDR < 0.01, log2FC > 2). Further analysis revealed that transcription factors (TFs) in plant hormone signal transduction pathway and plant pathogen interaction pathway, like WRKY22, GH3, TIFY/JAZ, ERF1, WRKY33, TGA, played vital role in fungal disease response. They mainly involved signal transduction, environmental adaptation, biosynthesis/metabolism of secondary metabolites. In addition, we found jasmonic acid/ethylene is the noteworthy signaling pathway in response to fungal disease in G. elata f. glauca. Conclusions The results reveal an underlying immune response mechanism of fungal disease in G. elata f. glauca and provide a novel insight into the breeding of disease resistant varieties of G. elata f. glauca.

Homokaryotic High-Quality Genome Assembly of Medicinal Fungi Wolfiporia hoelen Reveals Auto-Regulation and High-Temperature Adaption of Probable Two-Speed Genome

“…Phenylalanine, tyrosine, and tryptophan metabolism was involved in waterlogging and drought resisting of Chenopodium quinoa willd [31], while phenylalanine metabolism responded to cold stimulation in Brassica napus [32]. Phenylalanine and tyrosine metabolism was involved in disease resistance of Gastrodia elata f. glauca and rice [33,34]. Meanwhile, amino sugar and nucleotide sugar metabolism was also separately involved in drought resistance and disease resistance in Reaumuria soongorica and Poa pratensis [35,36].…”