Transcriptome analysis reveals the mechanism of the effect of flower tea <i>Coreopsis tinctoria</i> on hepatic insulin resistance

Jiang, Baoping; Lv, Qiuyue; Wan, Wenting; Le, Liang; Xu, Lijia; Hu, Keping; Xiao, Pei‐Gen

doi:10.1039/c8fo00965a

Cited by 15 publications

(10 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…RASD1, Ras-related dexamethasone induced 1, was an activator of G-protein signaling [ 40 ]. RASD1 was probably involved in hepatic insulin resistance [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

A nomogram for predicting metabolic steatohepatitis: The combination of NAMPT, RALGDS, GADD45B, FOSL2, RTP3, and RASD1

Liao

Zeng

et al. 2021

Open Medicine

View full text Add to dashboard Cite

Objective To identify differentially expressed and clinically significant mRNAs and construct a potential prediction model for metabolic steatohepatitis (MASH). Method We downloaded four microarray datasets, GSE89632, GSE24807, GSE63067, and GSE48452, from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) analysis and weighted gene co-expression network analysis were performed to screen significant genes. Finally, we constructed a nomogram of six hub genes in predicting MASH and assessed it through receiver operating characteristic (ROC) curve, calibration plot, and decision curve analysis (DCA). In addition, qRT-PCR was used for relative quantitative detection of RNA in QSG-7011 cells to further verify the expression of the selected mRNA in fatty liver cells. Results Based on common DEGs and brown and yellow modules, seven hub genes were identified, which were NAMPT, PHLDA1, RALGDS, GADD45B, FOSL2, RTP3, and RASD1. After logistic regression analysis, six hub genes were used to establish the nomogram, which were NAMPT, RALGDS, GADD45B, FOSL2, RTP3, and RASD1. The area under the ROC of the nomogram was 0.897. The DCA showed that when the threshold probability of MASH was 0–0.8, the prediction model was valuable to GSE48452. In QSG-7011 fatty liver model cells, the relative expression levels of NAMPT, GADD45B, FOSL2, RTP3, RASD1 and RALGDS were lower than the control group. Conclusion We identified seven hub genes NAMPT, PHLDA1, RALGDS, GADD45B, FOSL2, RTP3, and RASD1. The nomogram showed good performance in the prediction of MASH and it had clinical utility in distinguishing MASH from simple steatosis.

show abstract

“…RASD1, Ras-related dexamethasone induced 1, was an activator of G-protein signaling [ 40 ]. RASD1 was probably involved in hepatic insulin resistance [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

A nomogram for predicting metabolic steatohepatitis: The combination of NAMPT, RALGDS, GADD45B, FOSL2, RTP3, and RASD1

Liao

Zeng

et al. 2021

Open Medicine

View full text Add to dashboard Cite

show abstract

“…Hasil dari penelitian tersebut menunjukkan bahwa ekstrak mampu menurunkan resistensi insulin. 29 Selanjutnya, terdapat jenis tanaman teh hijau yang dapat menurunkan kadar glukosa darah, serum insulin, kadar HbA1c darah, hiperplasia serta ekspansi islet pankreas. 30 Sebanyak 1 artikel menjelaskan tikus yang diinduksi dengan menggunakan alloxan monohydrate dan larutan glukosa hingga tikus diabetes, kemudian dibagi menjadi kelompok kontrol (air), kelompok perlakuan (teh chamomile), dan kelompok standar (glibenklamid).…”

Section: Studi In Vivo Minuman Herbal Antidiabetesunclassified

Potensi Tanaman Herbal Antidiabetes untuk Minuman Obat: Sebuah Literatur Review

Prisdiany¹,

Puspitasari²,

Putriana³

et al. 2021

ijcp

View full text Add to dashboard Cite

Jumlah kasus dan prevalensi diabetes terus meningkat selama beberapa dekade terakhir. Pada tahun 2012, diabetes menyebabkan 1,5 juta kematian. Seiring berkembangnya pengetahuan, saat ini tanaman herbal lebih banyak digunakan sebagai bahan obat. Salah satu sediaan yang saat ini sedang banyak dikembangkan adalah sediaan minuman herbal. Minuman herbal kaya akan senyawa bioaktif alami. Bukti ilmiah menunjukkan bahwa senyawa bioaktif ini memberikan banyak efek biologis, seperti antioksidan, antibakteri, antidiabetes, dan lain-lain. Salah satu permasalahan yang berkaitan dengan penggunaan tanaman herbal adalah kurangnya penelitian atau pengujian secara ilmiah yang menunjukkan khasiat dari tanaman obat yang digunakan. Artikel ini memberikan gambaran secara ilmiah tentang tanaman herbal yang sudah terbukti memiliki khasiat antidiabetes yang dapat dimanfaatkan untuk pengembangan minuman obat herbal. Pencarian literatur melalui basis data elektronik PubMed pada bulan April 2020 dengan kata kunci "herbal tea" dan "antidiabetic" memperoleh total 30 artikel yang dapat ditelaah lebih lanjut. Sebanyak 12 artikel tentang uji in vitro, 16 artikel tentang uji in vivo, dan 2 artikel tentang uji klinik. Terdapat 35 jenis tanaman herbal di seluruh dunia yang terbukti dapat memberikan aktivitas antidiabetes. Bagian morfologi tanaman yang paling sering digunakan yaitu bagian daun. Mekanisme in vitro dari tanaman herbal dalam memberikan aktivitas antidiabetes adalah dengan menghambat aktivitas enzim α-Amylase dan α-Glucosidase. Pada uji in vivo dan uji klinik, semua tanaman herbal yang digunakan pada penelitian mempunyai aktivitas dalam membantu menurunkan kadar glukosa darah dan dapat dimanfaatkan sebagai minuman obat herbal.

show abstract

“…Forty male Wistar rats weighing 200 ± 20 g were purchased from Speyford (Beijing) Biotechnology Co., Ltd. All rats were maintained under a 12 h light/dark cycle at room temperature (22 ± 2 • C) with free access to food and water. The animal experiments were performed in accordance with the Guidelines and Policies for Animal Surgery under the approval of the Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (approval No: SLXD-20170302526) and were approved by the Institutional Animal Use and Care Committee (IACUC) (10).…”

Section: Animal Procedures and Drug Treatmentmentioning

confidence: 99%

“…Statistical significance was set at p < 0.05. The method used for network analysis was described in our previous work (10).…”

Section: Statistics and Network Analysismentioning

confidence: 99%

Liver Transcriptomic Reveals Novel Pathways of Empagliflozin Associated With Type 2 Diabetic Rats

Xiang

et al. 2020

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

The hypoglycaemic target of empagliflozin (EMP), as a novel inhibitor of sodium-glucose cotransporter (SGLT2), is clear. However, recent studies have shown that EMP also has an important role in lipid metabolism and cardiovascular diseases. The liver plays an important role in the development of type 2 diabetes (T2D), although whether EMP affects liver glucose metabolism is currently not reported. This study was designed to evaluate the effect of EMP on hepatic glucose metabolism in T2D and the underlying mechanism. A model of T2D was established by a high-fat and glucose diet (HFD) combined with streptozotocin (30 mg/kg) in male Wistar rats. Serum samples were collected to measure biochemical indicators, and liver samples were extracted for RNA-seq assay. Quantitative real-time PCR (qPCR) was used to further verify the gene expression levels detected by the RNA-seq assay. The EMP group showed significantly decreased blood glucose, triglyceride, cholesterol, non-esterified fatty acid and low-density lipoprotein cholesterol levels, and increased high-density lipoprotein cholesterol levels in serum compared with the type 2 diabetes model (MOD) group. Furthermore, EMP decreased the levels of inflammatory factors IL-1β, IL-6, and IL-8 in the serum compared to the MOD. Liver transcriptome analysis showed EMP affects a large number of upregulated and downregulated genes. Some of these genes are novel and involve in the metal ion binding pathway and the negative regulation of transcription from the RNA polymerase II promoter pathway, which are also closely related to glucolipid metabolism and insulin signaling. Our study provides new knowledge about the mechanism through which SGLT inhibitor can offer beneficial effects in T2D and especially in the hepatic metabolism. These genes found in this study also laid a solid foundation for further research on the new roles and mechanisms of EMP.

show abstract

Transcriptome analysis reveals the mechanism of the effect of flower tea Coreopsis tinctoria on hepatic insulin resistance

Abstract: Non-Camellia tea and herbal medicine help prevent the development of diabetes and other metabolic diseases.

Cited by 15 publications

References 64 publications

A nomogram for predicting metabolic steatohepatitis: The combination of NAMPT, RALGDS, GADD45B, FOSL2, RTP3, and RASD1

A nomogram for predicting metabolic steatohepatitis: The combination of NAMPT, RALGDS, GADD45B, FOSL2, RTP3, and RASD1

Potensi Tanaman Herbal Antidiabetes untuk Minuman Obat: Sebuah Literatur Review

Liver Transcriptomic Reveals Novel Pathways of Empagliflozin Associated With Type 2 Diabetic Rats

Contact Info

Product

Resources

About