Transcriptome analysis reveals the GRAS family in Salvia miltiorrhiza hairy roots in response to gibberellin

Abstract

Background: Salvia miltiorrhiza is one of the most important traditional Chinese medicinal plants with high medicinal value. Gibberellins are growth-promoting phytohormones that regulate numerous growth and developmental processes. However, their role in regulating secondary metabolism has not been investigated. Results: In this study, we found that GA can promote hairy roots growth and increase the content of tanshinones and phenolic acids. Transcriptome sequencing revealed that secondary metabolism pathway genes were enriched in the GA-responded. After further analysis of the changes of GA signaling pathway genes, it was found that the GRAS transcription factors family had a significant response to GA. We identified 35 SmGRAS genes in S. miltiorrhiza. SmGRAS genes can be divided into ten subfamilies in which members of the same subfamily showed similar conserved motifs and gene structures, suggesting the possible conserved functions.Conclusions: Most SmGRAS genes are significantly responsive to GA, indicating that they may play an important role in the GA signaling pathway and participate in GA regulation of root growth and secondary metabolism in S. miltiorrhiza.

Advances in Biotechnological Production and Metabolic Regulation of Astragalus membranaceus

“…The pathway of secondary metabolite biosynthesis can be explained by the identification of candidate genes and important regulatory factors. The best inducer for screening the Astragalus polysaccharide metabolic pathway is silver nitrate solution, the best treatment site is the underground part, the best treatment time is 6-9 days, and the best polysaccharide content change detection site is the Astragalus root; 36 unigenes were found in the metabolic pathway of the polysaccharides metabolized [46]. Studies have found that methyl jasmonate, salicylic acid, IAA, and NAA promote the accumulation of Astragalus hairy root polysaccharides and total saponins, and IBA and 2.4-D have a negative effect on the accumulation of Astragalus hairy root polysaccharides and total saponins [47].…”