Transcriptome analysis reveals the effects of strigolactone on shoot regeneration of apple

Asghar, Sumeera; Xiong, Yan; Che, Meng; Fan, Xingqiang; Li, Hui; Wang, Yi; Xu, Xuefeng; Li, Wei; Han, Zhenhai

doi:10.1007/s00299-022-02882-x

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…However, the same effect was not observed in the ER cultivar, indicating a cultivar-specific response mechanism. Similarly, recent reports have highlighted the positive impact of TIS108 on shoot regeneration in woody plants (Asghar et al, 2022).…”

Section: Discussionmentioning

confidence: 83%

Identification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Olea europaea L.)

Özbilen,

Sezer,

Taşkin

2024

Plant Direct

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Strigolactones (SLs), synthesized in plant roots, play a dual role in modulating plant growth and development, and in inducing the germination of parasitic plant seeds and arbuscular mycorrhizal fungi in the rhizosphere. As phytohormones, SLs are crucial in regulating branching and shaping plant architecture. Despite the significant impact of branching strategies on the yield performance of fruit crops, limited research has been conducted on SLs in these crops. In our study, we identified the transcript sequences of SL biosynthesis and signaling genes in olive (Olea europaea L.) using rapid amplification of cDNA ends. We predicted the corresponding protein sequences, analyzed their characteristics, and conducted molecular docking with bioinformatics tools. Furthermore, we quantified the expression levels of these genes in various tissues using quantitative real‐time PCR. Our findings demonstrate the predominant expression of SL biosynthesis and signaling genes (OeD27, OeMAX3, OeMAX4, OeMAX1, OeD14, and OeMAX2) in roots and lateral buds, highlighting their importance in branching. Treatment with rac‐GR24, an SL analog, enhanced the germination frequency of olive seeds in vitro compared with untreated embryos. Conversely, inhibition of SL biosynthesis with TIS108 increased lateral bud formation in a hard‐to‐root cultivar, underscoring the role of SLs as phytohormones in olives. These results suggest that modifying SL biosynthesis and signaling pathways could offer novel approaches for olive breeding, with potential applicability to other fruit crops.

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Section: Discussionmentioning

confidence: 83%

Identification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Olea europaea L.)

Özbilen,

Sezer,

Taşkin

2024

Plant Direct

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“…To build the gene overexpression vector, the coding sequences of MdBRC1 , MdSMXL7 , MdWRKY6 and MdGH3.1 without a stop codon were cloned and inserted into pRI101‐eGFP, respectively. A previously built MdCCD7 ‐overexpressing vector (Asghar et al., 2022) was used in the present study. To construct the MdBRC1 ‐RNAi vector, a 244‐bp region of the MdBRC1 coding sequence and its reverse complement sequence were cloned and inserted on opposite sides of an intron into the same pCAMBIA1305.1 vector (Figure S8).…”

Section: Methodsmentioning

confidence: 99%

Strigolactone regulates adventitious root formation via the MdSMXL7‐MdWRKY6‐MdBRC1 signaling cascade in apple

Fan

Deng

et al. 2023

The Plant Journal

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SUMMARY Propagation through stem cuttings is a popular method worldwide for species such as fruit tree rootstocks and forest trees. Adventitious root (AR) formation from stem cuttings is crucial for effective and successful clonal propagation of apple rootstocks. Strigolactones (SLs) are newly identified hormones involved in AR formation. However, the regulatory mechanisms underpinning this process remain elusive. In the present study, weighted gene co‐expression network analysis, as well as rooting assays using stable transgenic apple materials, revealed that MdBRC1 served as a key gene in the inhibition of AR formation by SLs. We have demonstrated that MdSMXL7 and MdWRKY6 synergistically regulated MdBRC1 expression, depending on the interactions of MdSMXL7 and MdWRKY6 at the protein level downstream of SLs as well as the direct promoter binding on MdBRC1 by MdWRKY6. Furthermore, biochemical studies and genetic analysis revealed that MdBRC1 inhibited AR formation by triggering the expression of MdGH3.1 in a transcriptional activation pathway. Finally, the present study not only proposes a component, MdWRKY6, that enables MdSMXL7 to regulate MdBRC1 during the process of SL‐controlled AR formation in apple, but also provides prospective target genes to enhance AR formation capacity using CRISPR (i.e. clustered regularly interspaced short palindromic repeats) technology, particularly in woody plants.

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“…SMAX1/SMXL genes, members of a small gene family, function as downstream suppressors of the D14-dependent strigolactone signaling pathway and KAI2-dependent KAR/KL signaling pathway [23][24][25][26]28]. Our knowledge about genes downstream of SMAX1/SMXL is still limited, even in angiosperm species [46][47][48]. This study revealed that GCAM1 works in the downstream genetic cascade of the KAI2-dependent signaling pathway in M. polymorpha.…”

Section: Gcam1 Work Downstream Of the Kai2-dependent Signaling To Con...mentioning

confidence: 99%

KARRIKIN INSENSITIVE2(KAI2)-dependent signaling pathway controls vegetative reproduction inMarchantia polymorpha

Komatsu

Kodama

Mizuno

et al. 2022

Preprint

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In vegetative reproduction of Marchantia polymorpha, propagules, called gemmae, are formed in gemma cups. Despite its significance for survival, control of gemma and gemma cup formation by environmental cues is not well understood. We show here that the number of gemmae formed in a gemma cup is a genetic trait. Gemma formation starts from the central region of the floor of the gemma cup, proceeds to the periphery, and terminates when the appropriate number of gemmae are initiated. The MpKARRIKIN INSENSITIVE2 (MpKAI2)-dependent signaling pathway promotes gemma cup formation and gemma initiation. The number of gemmae in a cup is controlled by modulating the ON/OFF switch of the KAI2-dependent signaling. Termination of the signaling results in the accumulation of MpSMXL, a suppressor protein. In the Mpsmxl mutants, gemma initiation continues, leading to the formation of a highly increased number of gemmae in a cup. Consistent with its function, the MpKAI2-dependent signaling pathway is active in gemma cups where gemmae initiate, as well as in the notch region of the mature gemma and midrib of the ventral side of the thallus. In this work, we also show that GEMMA CUP-ASSOCIATED MYB1 works downstream of this signaling pathway to promote gemma cup formation and gemma initiation. We also found that the availability of potassium affects gemma cup formation independently from the KAI2-dependent signaling pathway in M. polymorpha. We propose that the ancestral function of the KAI2-dependent signaling pathway may be to optimize vegetative reproduction by adapting to the environment.

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Transcriptome analysis reveals the effects of strigolactone on shoot regeneration of apple

Cited by 5 publications

References 57 publications

Identification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Olea europaea L.)

Identification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Olea europaea L.)

Strigolactone regulates adventitious root formation via the MdSMXL7‐MdWRKY6‐MdBRC1 signaling cascade in apple

KARRIKIN INSENSITIVE2(KAI2)-dependent signaling pathway controls vegetative reproduction inMarchantia polymorpha

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