Transcriptome analysis reveals the accumulation mechanism of anthocyanins in ‘Zijuan’ tea (Camellia sinensis var. asssamica (Masters) kitamura) leaves

Li, Jian; Lv, Xiaoyang; Wang, Lingxia; Qiu, Zhimin; Song, Xiaomin; Lin, Juan; Chen, Wei

doi:10.1007/s10725-016-0183-x

Cited by 48 publications

(45 citation statements)

References 54 publications

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“…A previous report indicated that both PAL expression and PAL activities were upregulated in purple leaves of the Zijuan tea tree [16]. In our proteomics data, PAL abundance in the purple leaves was slightly higher (1.3-fold) as compared to that in the green leaves, but the difference was insignificant.…”

Section: Discussioncontrasting

confidence: 53%

Regulation of Anthocyanin Biosynthesis in Purple Leaves of Zijuan Tea (Camellia sinensis var. kitamura)

Wang

Pan

Liang

et al. 2017

IJMS

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Plant anthocyanin biosynthesis is well understood, but the regulatory mechanism in purple foliage tea remains unclear. Using isobaric tag for relative and absolute quantification (iTRAQ), 815 differential proteins were identified in the leaves of Zijuan tea, among which 20 were associated with the regulation of anthocyanin metabolism. We found that the abundances of anthocyanin synthesis-related enzymes such as chalcone synthase, chalcone isomerase, dihydroflavonol 4-reductase and anthocyanin synthetase, as well as anthocyanin accumulation-related UDP-glucosyl transferase and ATP-binding cassette (ABC) transporters in the purple leaves were all significantly higher than those in the green leaves. The abundances of the transcription factors bHLH and HY5, regulating anthocyanin biosynthesis at transcriptional level were also obviously higher in purple leaves than those in green leaves. In addition, bifunctional 3-dehydroquinate dehydratase and chorismate mutase in purple leaves were distinctly higher in abundance compared to green leaves, which provided sufficient phenylalanine substrate for anthocyanin synthesis. Furthermore, lignin synthesis was found to be reduced due to the lower abundances of cinnamoyl-CoA reductase 1, peroxidase 15 and laccase-6, which resulted in increase of intermediates flow into anthocyanin synthesis pathway. The physiological data were consistent with proteomic results. These four aspects of biosynthetic regulation contribute to anthocyanin accumulation in purple leaves of Zijuan tea.

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Section: Discussioncontrasting

confidence: 53%

Regulation of Anthocyanin Biosynthesis in Purple Leaves of Zijuan Tea (Camellia sinensis var. kitamura)

Wang

Pan

Liang

et al. 2017

IJMS

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“…Under drought stress conditions, the accumulation of anthocyanins appears to be under complex regulatory control at both spatial and temporal levels and thus the inductive mechanisms of anthocyanin synthesis remain unresolved (Castellarin et al 2007a;Ollé et al 2011). Abscisic acid (ABA) is a plant hormone that regulates plant growth, development such as seed dormancy, floral induction, and is involved in abiotic stress responses such as drought stress, salinity, and cold (Finkelstein 2013;Li et al 2017a). Under these abiotic stresses, ABA regulates the activation of antioxidant enzymes and also reduces stomatal aperture (Choudhary et al 2011;Guajardo et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

González‐Villagra

Kurepin

2017

Planta

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Main conclusion ABA is involved in anthocyanin synthesis through the regulation of microRNA156, augmenting the level of expression of anthocyanin synthesisrelated genes and, therefore, increasing anthocyanin level.Drought stress is the main cause of agricultural crop loss in the world. However, plants have developed mechanisms that allow them to tolerate drought stress conditions. At cellular level, drought stress induces changes in metabolite accumulation, including increases in anthocyanin levels due to upregulation of the anthocyanin biosynthetic pathway. Recent studies suggest that the higher anthocyanin content observed under drought stress conditions could be a consequence of a rise in the abscisic acid (ABA) concentration. This plant hormone crosses the plasma membrane by specific transporters, and it is recognized at the cytosolic level by receptors known as pyrabactin resistance (PYR)/regulatory component of ABA receptors (PYR/RCARs) that regulate downstream components. In this review, we discuss the hypothesis regarding the involvement of ABA in the regulation of microRNA156 (miRNA156), which is upregulated as part of dehydration stress responsiveness in different species. The miRNA156 upregulation produces a greater level of anthocyanin gene expression, forming the multienzyme complex that will synthesize an increased level of anthocyanins at the cytosolic face of the rough endoplasmic reticulum (RER). After synthesis, anthocyanins are transported from the RER to the vacuole by two possible models of transport: (1) membrane vesicle-mediated transport, or (2) membrane transporter-mediated transport. Thus, the aim was to analyze the recent findings on synthesis, transport and the possible mechanism by which ABA could increase anthocyanin synthesis under drought stress conditions potentially throughout microRNA156 (miRNA156).

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“…In a recent study on Camellia sinensis var. assamica it was observed that the expression of anthocyanin biosynthetic genes such as DFR can be regulated by the induction of various transcriptions factors and hence induces higher anthocyanin accumulation 35 . A similar mechanism may be conjectured to occur in plants treated with heavy metal stress.…”

Section: Fig 3amentioning

confidence: 99%

Blood chemistry profile of Bali cattle fed silage rice straw and biosupplement of selected rumen and termites lignocellulolytic bacteria consortium

2017

JCBSC

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Transcriptome analysis reveals the accumulation mechanism of anthocyanins in ‘Zijuan’ tea (Camellia sinensis var. asssamica (Masters) kitamura) leaves

Cited by 48 publications

References 54 publications

Regulation of Anthocyanin Biosynthesis in Purple Leaves of Zijuan Tea (Camellia sinensis var. kitamura)

Regulation of Anthocyanin Biosynthesis in Purple Leaves of Zijuan Tea (Camellia sinensis var. kitamura)

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

Blood chemistry profile of Bali cattle fed silage rice straw and biosupplement of selected rumen and termites lignocellulolytic bacteria consortium

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