Transcriptome analysis reveals that high temperatures alter modes of lipid metabolism in juvenile turbot (Scophthalmus maximus) liver

Zhao, Tingting; Ma, Aijun; Huang, Zhihui; Liu, Zhifeng; Sun, Zhibin; Zhu, Chunyue; Yang, Jingkun; Li, Yingdi; Wang, Qingmin; Qiao, Xuewei; Chen, Zhuo

doi:10.1016/j.cbd.2021.100887

Cited by 21 publications

(14 citation statements)

References 53 publications

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“…Lipid metabolism, as an important component of energy metabolism, participates in many important physiological processes (Luo et al, 2021; Zhao et al, 2021). Studies have shown that lipid metabolism is the main form of energy consumption of shrimp under ammonia exposure, and the levels of TG and T‐CHO are usually used as indicators of lipid metabolism (Li, Zhang et al, 2019; Li, Ma et al, 2019; Racotta & Hernández‐Herrera, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Lipid metabolism provides the energy to ensure the normal functioning of physiological operations in aquatic animals (Chen et al, 2014). However, the action of external environmental factors, such as viruses, temperature and copper, can lead to fat metabolism disorders (Cheng & Wang, 2016; Liu et al, 2022; Zhao et al, 2021). Previous studies on ammonia in lipid metabolism have been carried out in Penaeus vannamei , Litopenaeus vannamei and Piaractus mesopotamicus (Carneiro et al, 2021; Wang et al, 2020, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of lipid metabolism between broodstock and hybrid offspring in the hepatopancreas of juvenile shrimp (Macrobrachium nipponense): Response to chronic ammonia stress

Jiang

et al. 2022

Animal Genetics

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Ammonia nitrogen is a major pollutant that causes great physiological harm to crustaceans in culture. In this study, we conducted a 28 day chronic ammonia nitrogen stress experiment with broodstock populations (Dianshan, DS) and hybrid offspring populations (DS ♀ × CD (Changjiang ♂ × Dongting ♀), SCD) exposed to 0, 1 and 10 mg/L of ammonia concentrations. A 28 day feeding trial and chronic ammonia nitrogen stress were used to investigate the effects on the growth performance, histological structure and lipid metabolism of juvenile shrimp, Macrobrachium nipponense. Our results indicated that survival rates in the SCD groups were significantly higher than those in the DS groups, whereas weight and length gain rates were not significantly different between the groups (p > 0.05). Histological structure results showed that the number of vacuoles in the DS group was significantly higher than that in the SCD group and hepatopancreas cell structures were disrupted in the ammonia treatment groups. The results of oil red staining showed that the number of lipid droplets increased significantly with the increase in ammonia concentration. As the ammonia concentration increased, fatty acid contents, lipid enzyme activities and lipid metabolism‐related gene expression all tended to rise. In conclusion, ammonia nitrogen exposure caused damage to the hepatopancreas structure of juvenile shrimp and disturbed the lipid metabolism of the hepatopancreas. In addition, the SCD population had stronger stress resistance than the DS population when subjected to the same concentration of ammonia nitrogen stress.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of lipid metabolism between broodstock and hybrid offspring in the hepatopancreas of juvenile shrimp (Macrobrachium nipponense): Response to chronic ammonia stress

Jiang

et al. 2022

Animal Genetics

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“…In the SRA study of SRP152627, kidney tissue in turbot exposed to heat stress (23°C, 25°C, and 28°C) for 24 h and kidney tissue in the control group maintained at 14°C was used for RNA-seq (Huang et al, 2020). In the SRA study of SRP273870, RNA-seq was conducted on liver tissue in the treatment group under heat stress (20°C, 24°C, and 28°C) for 24 h and in the control group maintained at 14°C (Zhao et al, 2021). The SRA study of SRP167318 was used for oxygenstress expression analysis, in which gill tissues in turbot subjected to waterless treatment under different oxygen conditions (6.5 mg/L (water, control group), 300 mg/L (waterless, air group), and 1430 mg/L (waterless, oxygen)) were used for RNA-seq (Nie et al, 2020).…”

Section: Rna-seq Data Sets Under Abiotic Stressmentioning

confidence: 99%

Genome-wide identification of the MAPK gene family in turbot and its involvement in abiotic and biotic stress responses

Zheng

Xu²,

Zechen³

et al. 2022

Front. Mar. Sci.

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The mitogen-activated protein kinase (MAPK) gene family performs crucial roles in cell division, migration, development, apoptosis, inflammatory response, and abiotic and biotic stress responses. However, very little information is available about the MAPKs in turbot (Scophthalmus maximus). In this study, 15 turbot MAPKs (SmMAPKs) were identified throughout the whole genome, and their basic chemical and physical properties and subcellular localization were illustrated. All SmMAPKs contained the serine/threonine protein kinases, catalytic domain (S_TKc, SMART00220). Phylogenetic analysis revealed that SmMAPKs were classified into three subfamilies, namely, c-Jun NH2-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and p38. Conserved motif and gene structure analysis revealed high levels of conservation within and between phylogenetic subfamilies. Expression patterns of MAPKs in distinct tissues and under diverse abiotic and biotic stresses were examined using the published available RNA-seq data sets. As a result, SmMAPKs showed obviously tissue-specific expression. Furthermore, 7 and 10 candidate stress-responsive MAPK genes were detected under abiotic and biotic stresses, respectively, among which five common MAPK genes, namely, SmMAPK4 (ERK4), SmMAPK6 (ERK3), SmMAPK11 (p38β), SmMAPK12b (p38γ), and SmMAPK15 (ERK7/8) showed extremely significant responses to both abiotic and biotic stresses, demonstrating their potential functions in comprehensive antistress. These results demonstrate that MAPKs might play vital roles in response to various abiotic and biotic stresses in turbot, which would contribute to making scientific preventive measures to environmental changes in the process of farming and promoting the development of selective breeding for comprehensive stress resistance in turbot.

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“…Indeed, global warming poses a serious threat to global aquaculture production [ 4 ]. Rising water temperature has a series of deleterious effects on the immunity [ 2 , 5 ], metabolism [ 6 , 7 ], reproduction and development [ 8 , 9 ], and behavioral patterns [ 10 , 11 ] of aquatic organisms.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Gut Microbiome in the Mud Snail Cipangopaludina cathayensis in Response to High-Temperature Stress

Cheng

Liu

et al. 2022

Animals

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The mud snail Cipangopaludina cathayensis is a widely distributed species in China. Particularly in Guangxi province, mud snail farming contributes significantly to the economic development. However, global warming in recent decades poses a serious threat to global aquaculture production. The rising water temperature is harmful to aquatic animals. The present study explored the effects of high temperature on the intestinal microbiota of C. cathayensis. Snail intestinal samples were collected from the control and high-temperature groups on days 3 and 7 to determine the gut microbiota composition and diversity. Gut bacterial community composition was investigated using high-throughput sequencing of the V3–V4 region of bacterial 16S rRNA genes. Our results suggested that thermal stress altered the gut microbiome structure of C. cathayensis. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were dominant in C. cathayensis gut microbiota. The T2 treatment (32 ± 1 °C, day 7) significantly decreased the relative abundance of Firmicutes, Actinobacteria, and Deinococcus-Thermus. In T2, the abundance of several genera of putatively beneficial bacteria (Pseudomonas, Aeromonas, Rhodobacter, and Bacteroides) decreased, whereas the abundance of Halomonas—a pathogenic bacterial genus—increased. The functional prediction results indicated that T2 treatment inhibited some carbohydrate metabolism pathways and induced certain disease-related pathways (e.g., those related to systemic lupus erythematosus, Vibrio cholerae infection, hypertrophic cardiomyopathy, and shigellosis). Thus, high temperature profoundly affected the community structure and function of C. cathayensis gut microbiota. The results provide insights into the mechanisms associated with response of C. cathayensis intestinal microbiota to global warming.

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Transcriptome analysis reveals that high temperatures alter modes of lipid metabolism in juvenile turbot (Scophthalmus maximus) liver

Cited by 21 publications

References 53 publications

Comparison of lipid metabolism between broodstock and hybrid offspring in the hepatopancreas of juvenile shrimp (Macrobrachium nipponense): Response to chronic ammonia stress

Comparison of lipid metabolism between broodstock and hybrid offspring in the hepatopancreas of juvenile shrimp (Macrobrachium nipponense): Response to chronic ammonia stress

Genome-wide identification of the MAPK gene family in turbot and its involvement in abiotic and biotic stress responses

Characterization of Gut Microbiome in the Mud Snail Cipangopaludina cathayensis in Response to High-Temperature Stress

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