Transcriptome Analysis Reveals Silver Nanoparticle-Decorated Quercetin Antibacterial Molecular Mechanism

Abstract

Facile and simple method is developed to synthesize silver-nanoparticle-decorated quercetin nanoparticles (QA NPs). Modification suggests that synergistic quercetin (Qe) improves the antibacterial effect of silver nanoparticles (Ag NPs). Characterization experiment indicates that QA NPs have a diameter of approximately 10 nm. QA NPs show highly effective antibacterial activities against drug-resistant Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). We explore antibacterial mechanisms using S. aureus and E. coli treated with QA NPs. Through morphological changes in E. coli and S. aureus, mechanisms are examined for bacterial damage caused by particulate matter from local dissociation of silver ion and Qe from QA NPs trapped inside membranes. Moreover, we note that gene expression profiling methods, such as RNA sequencing, can be used to predict discover mechanisms of toxicity of QA NPs. Gene ontology (GO) assay analyses demonstrate the molecular mechanism of the antibacterial effect of QA NPs. Regarding cellular component ontology, "cell wall organization or biogenesis" (GO: 0071554) and "cell wall macromolecule metabolic process" (GO: 0044036) are the most represented categories. The present study reports that transcriptome analysis of the mechanism offers novel insights into the molecular mechanism of antibacterial assays.

Biosynthesis of silver nanoparticle from pomelo (Citrus Maxima) and their antibacterial activity against acidovorax oryzae RS-2

“…Interestingly, bacterial cells indicated a mixture of red and green fluoresce when exposed to AgNPs ( figure 10(c)). However, the number of cells was highly reduced in the presence of AgNPs which indicated an inhibition of bacterial growth and replication, which is consistent with previous studies [56,57]. Therefore, it seems that AgNPs has a bacteriostatic as well as bactericidal effect on Ao strain RS-2.…”

Designing Aptamer-Gold Nanoparticle-Loaded pH-Sensitive Liposomes Encapsulate Morin for Treating Cancer

“…All cells (including the floating cells in the medium) were collected with 2000 r/ min for 5 min. The collected cells were then post-fixed in 2.5% glutaric dialdehyde solution for 4 h [32]. The fixed cells were dehydrated in an acetone gradient series for 20 min.…”

Peer Review #2 of "The anti-biofilm effect of silver-nanoparticle-decorated quercetin nanoparticles on a multi-drug resistant Escherichia coli strain isolated from a dairy cow with mastitis (v0.1)"

“…In recent years, nanoparticles, safe, cost effective bactericidal materials, which are gaining popularity, and have been used successfully as carriers of therapeutic agents, in diagnostics of chronic diseases (Gomes & Henriques 2016;Hong et al 2008;Martinez-Gutierrez et al 2013;Zhang et al 2008). In addition, although silver is toxic to microorganisms, it is less dangerous to mammalian cells than other metals (Morones et al 2005;Sondi & Salopeksondi 2004;Sun et al 2017;Zhao & Jr 1998). Silver nanoparticles (Ag NPs or nanosilver), a kind of nanosized silver particle, are widely used NPs and show strong biocidal effects on a broad spectrum of bacterial pathogens, including E. coli (Morones et al 2005;Sondi & Salopeksondi 2004).…”

“…Hence, the synergistic effect of Ag NPs and other antimicrobial agents, especially plant-derived drugs, has gotten a lot of attention (Gomes & Henriques 2016). Previous reports show that plant-derived drugs have the advantage of not inducing resistance after prolonged exposure and are easily obtained (Domadia et al 2007;Ohno et al 2003;Sun et al 2017). 3,3′,4′,5,7-Pentahydroxyflavone (quercetin, Qe), a kind of flavonoid compound, is Manuscript to be reviewed commonly present in various foods including onions, fruits, and vegetables (Smith et al 2016;Sun et al 2017).…”

“…Previous reports show that plant-derived drugs have the advantage of not inducing resistance after prolonged exposure and are easily obtained (Domadia et al 2007;Ohno et al 2003;Sun et al 2017). 3,3′,4′,5,7-Pentahydroxyflavone (quercetin, Qe), a kind of flavonoid compound, is Manuscript to be reviewed commonly present in various foods including onions, fruits, and vegetables (Smith et al 2016;Sun et al 2017). Silver-nanoparticle-decorated quercetin nanoparticles (QA NPs) showed highly effective antibacterial activity against drug-resistant E. coli (Sun et al 2017).…”