Transcriptome analysis reveals<scp>TOR</scp>signalling‐mediated plant flush shoots governing<scp><i>Diaphorina citri</i>Kuwayama</scp>oviposition

Guo, Shu-Hao; Liu, Y.-M.; Wang, Z.-Y.; Wang, F.-F.; Mao, Yan; Hu, Yingtong; Han, Pei‐Jie; Cuthbertson, Andrew G. S.; Qiu, Bao‐Li; Sang, Wen

doi:10.1111/imb.12693

Cited by 4 publications

(9 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In ACPs feeding on young shoots, psyllid female ovarian development was activated, inducing oviposition. However, silencing of the positive regulatory gene DcRheb in the target of rapamycin (TOR) pathway lowered ecdysone and juvenile hormone levels, suppressed vitellogenin synthesis, and reduced female reproductive capacity [86]. Genes in the TOR pathway, among others, have been proposed as the potent candidates for silencing to reduce the reproductive capacity of insects; however, if product of choice is an RNAi-based pesticide, it must be applied to shoots for successfully silencing the target transcripts.…”

Section: Acp Control Using Rnai Technologymentioning

confidence: 99%

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Marques¹,

Angelotti-Mendonça

Roberto

2021

Horticulturae

View full text Add to dashboard Cite

Citrus species, including sweet oranges, grapefruits, pomelos, and lemons, are the most widely cultivated trees and consumed fruits worldwide. In citrus orchard management, the control of Huanglongbing (HLB) disease and its insect vector Diaphorina citri (Asian citrus psyllid, ACP) represents a major global challenge. Consumers have been increasingly pushing the citrus production chain toward a more sustainable system, including stringent measures to prevent the use of chemical pesticides. In recent years, biotechnological advances have offered safe and environmentally friendly alternatives for crop production. Technologies such as RNA interference (RNAi)-mediated gene silencing have emerged as innovative tools for agricultural pest management. Here, we provide an overview of RNAi as a promising approach for ACP control and discuss the associated challenges. Despite the availability of specific silencing sequences aimed at a target gene of the insect pest, the uptake of double-stranded RNA is limited in hemipteran insects. In this context, improved delivery methods, stability maintenance, and RNAi response are considered the factors contributing to the increased effectiveness of exogenous RNAi against hemipteran pests. These approaches can serve as potential tools for efficient ACP control.

show abstract

Section: Acp Control Using Rnai Technologymentioning

confidence: 99%

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Marques¹,

Angelotti-Mendonça

Roberto

2021

Horticulturae

View full text Add to dashboard Cite

show abstract

“…The D. citri used in this study was collected on shrubs of Murraya paniculata near South China Agricultural University (SCAU), Guangzhou, China, and subcultured in the laboratory of the Engineering Research Center for Biological Control, Ministry of Education, SCAU. The culturing method was to simply allow the D. citri to feed on the host plant M. paniculata covered in a white nylon net bag in an incubator at 26 °C, 14 h light and 10 h dark photoperiod, and 60% ± 2% relative humidity (Guo et al ., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…These substances will be ingested by female D. citri , so changing their nutritional status after feeding on flush shoots (Sétamou et al ., 2016 ) and inducing them to lay eggs. Our previous research demonstrated that the expression levels of JH‐ and 20E‐related genes and VG increase after D. citri has fed on flush shoots (Guo et al ., 2021 ). The DcRheb (Ras‐homologue enriched in brain), DcRaptor (regulatory associated protein of mTOR), DcSin1 (target of rapamycin [TOR] complex 2 subunit MAPKAP1), and DcS6K (TOR complex 2 subunit MAPKAP1) genes in the TOR signaling pathway are also all upregulated after mated D. citri females have fed on flush shoots (Guo et al ., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Our previous research demonstrated that the expression levels of JH‐ and 20E‐related genes and VG increase after D. citri has fed on flush shoots (Guo et al ., 2021 ). The DcRheb (Ras‐homologue enriched in brain), DcRaptor (regulatory associated protein of mTOR), DcSin1 (target of rapamycin [TOR] complex 2 subunit MAPKAP1), and DcS6K (TOR complex 2 subunit MAPKAP1) genes in the TOR signaling pathway are also all upregulated after mated D. citri females have fed on flush shoots (Guo et al ., 2021 ). We also previously determined that the synthesis and effect of JH and 20E are closely related to the TOR pathway.…”

Section: Introductionmentioning

confidence: 99%

“…The expression of JH‐ and 20E‐related genes was significantly inhibited by interference treatment of DcRheb , a key factor in the TOR pathway. These results suggested that feeding on flush shoots induces oviposition in female D. citri and is realized by the TOR signaling pathway affecting JH and 20E (Guo et al ., 2021 ). However, its upstream pathway has not been fully studied.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Insulin peptides and their receptors regulate ovarian development and oviposition behavior in Diaphorina citri

et al. 2022

Self Cite

View full text Add to dashboard Cite

Diaphorina citri is an important vector of Citrus Huanglongbing (HLB) disease. After feeding on young host plant shoots, the population of D. citri can increase significantly. Females also only lay eggs on young shoots. However, there are few studies on the mechanism of this phenomenon. Exogenous nutrient signals can affect the insulin signaling system of D. citri after feeding on young shoots. In this study, the expression of upstream factors DcILP1, DcILP2, and DcIR in the insulin signaling system of D. citri was upregulated after feeding on young shoots. After being silenced by RNA interference technology, the results showed that the number of oviposited eggs of D. citri was significantly decreased and the ovarian development was inhibited with severe vacuolation. In addition, detection using quantitative reverse transcription-polymerase chain reaction showed that the upstream regulatory gene DcRheb of the target of rapamycin (TOR) pathway and the downstream reproduction-related DcVg gene were also significantly downregulated. These results suggest that feeding upon young shoots may upregulate the expression levels of upstream factors DcILP1, DcILP2, and DcIR in the insulin signaling system. The signal will be through upregulating the expression of DcRheb, an upstream gene of the TOR signaling pathway. This in turn influences yolk metabolism, which eventually causes the ovaries of female D. citri to mature and therefore initiate oviposition behavior.

show abstract

RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata

Li,

Zhang

et al. 2023

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUNDInsulin signalling pathways play crucial roles in regulating growth and development in insects, but their effects on the growth and development of Arachnids, such as spiders, have rarely been studied. As a valuable pest natural enemy in agricultural fields, the molecular mechanisms of insulin signalling pathway‐mediated growth and development of the wolf spider, Pardosa pseudoannulata, are of particular interest.RESULTSIn this study, we identified and characterized six insulin signalling pathway genes—InR, InR2, IRS1, PI3K1, PI3K2, and PDK—in P. pseudoannulata. Real‐time quantitative PCR results were used to analyse the relative expression levels of the six genes in different developmental instars and tissues, and in response to starvation treatment. In addition, the function of the insulin receptor substrate (IRS1) gene was investigated using RNA interference technology, which found that IRS1 significantly influenced nutrient content, developmental duration, body weight, and gonad development.CONCLUSIONThis study revealed the roles of six key insulin signalling pathway genes in P. pseudoannulata, and in particular the importance of the IRS1 gene in regulating growth and development in the spider. The results lay the foundation for further research on the internal regulation mechanisms of growth and development in Araneae species, and also provide a reference for the artificial breeding of spiders.This article is protected by copyright. All rights reserved.

show abstract

Transcriptome analysis revealsTORsignalling‐mediated plant flush shoots governingDiaphorina citriKuwayamaoviposition

Cited by 4 publications

References 56 publications

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Advances and Challenges in RNA Interference Technology for Citrus Huanglongbing Vector Control

Insulin peptides and their receptors regulate ovarian development and oviposition behavior in Diaphorina citri

RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata

Contact Info

Product

Resources

About