Transcriptome analysis reveals reasons for the low tolerance of Clostridium tyrobutyricum to furan derivatives

Suo, Yukai; Li, Wenyi; Wan, Liqiong; Luo, Linshuang; Liu, Shuang; Qin, Shiwen; Wang, Jufang

doi:10.1007/s00253-022-12281-7

Cited by 8 publications

(7 citation statements)

References 55 publications

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“…In previous studies, the effects of components of lignocellulosic hydrolysate on microbial activity were evaluated based on the levels of metabolic intermediates and gene expression as well as cell growth. 11,12 These results suggest that benzoic acids and cinnamic acid derivatives are critical fermentation inhibitors for 4APhe production from the enzymatic hydrolysate of sorghum bagasse and that the inhibitory effect of furfural and 5HMF on 4APhe production by E. coli is limited, even after repeated feeding of the hydrolysate.…”

Section: Resultsmentioning

confidence: 93%

“…These results suggest that 4APhe production from the enzymatic hydrolysate of sorghum bagasse by metabolically engineered E. coli was subjected to fermentation inhibition. 11,36…”

Section: Resultsmentioning

confidence: 99%

“…However, this hydrolysate also contains organic contaminants derived from the decomposition of cellulose, hemicellulose, and lignin, which can act as fermentation inhibitors that hinder cell growth and metabolism during fermentation. 1,11,12 Although a metabolically engineered Escherichia coli strain expressing papABC derived from Pseudomonas uorescens was developed and found to successfully produce 4APhe from glucose, [13][14][15][16] it was not able to produce 4APhe from lignocellulosic biomass (Table 1).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

et al. 2023

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Section: Resultsmentioning

confidence: 93%

“…These results suggest that 4APhe production from the enzymatic hydrolysate of sorghum bagasse by metabolically engineered E. coli was subjected to fermentation inhibition. 11,36…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

et al. 2023

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“…Insertion and overexpression of certain components of efflux pumps from less susceptible strains might considerably enhance tolerance to different inhibitors [ 59 ]. Moreover, genes for efflux pumps are commonly upregulated in response to lignocellulose-derived inhibitors and among them those of the ABC type [ 38 , 60 ]. The highest efflux pump activity during ABE fermentation was detected just prior to the metabolic switch, with a predominant representation of ABC-type pumps [ 61 ], which is in agreement with our observation that late acidogenic stages belong to the least susceptible stages to inhibitor impacts.…”

Section: Discussionmentioning

confidence: 99%

Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation

Branska,

Koppova,

Husakova

et al. 2024

Biotechnol Biofuels

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Background Inhibitors that are released from lignocellulose biomass during its treatment represent one of the major bottlenecks hindering its massive utilization in the biotechnological production of chemicals. This study demonstrates that negative effect of inhibitors can be mitigated by proper feeding strategy. Both, crude undetoxified lignocellulose hydrolysate and complex medium supplemented with corresponding inhibitors were tested in acetone–butanol–ethanol (ABE) fermentation using Clostridium beijerinckii NRRL B-598 as the producer strain. Results First, it was found that the sensitivity of C. beijerinckii to inhibitors varied with different growth stages, being the most significant during the early acidogenic phase and less pronounced during late acidogenesis and early solventogenesis. Thus, a fed-batch regime with three feeding schemes was tested for toxic hydrolysate (no growth in batch mode was observed). The best results were obtained when the feeding of an otherwise toxic hydrolysate was initiated close to the metabolic switch, resulting in stable and high ABE production. Complete utilization of glucose, and up to 88% of xylose, were obtained. The most abundant inhibitors present in the alkaline wheat straw hydrolysate were ferulic and coumaric acids; both phenolic acids were efficiently detoxified by the intrinsic metabolic activity of clostridia during the early stages of cultivation as well as during the feeding period, thus preventing their accumulation. Finally, the best feeding strategy was verified using a TYA culture medium supplemented with both inhibitors, resulting in 500% increase in butanol titer over control batch cultivation in which inhibitors were added prior to inoculation. Conclusion Properly timed sequential feeding effectively prevented acid-crash and enabled utilization of otherwise toxic substrate. This study unequivocally demonstrates that an appropriate biotechnological process control strategy can fully eliminate the negative effects of lignocellulose-derived inhibitors. Graphical Abstract

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“…They can serve as nutrients or inhibitors for downstream fermentation. For example, organic acids may be accumulated by metabolic activities of microorganisms during enzyme production, lead to changes in the pH of the medium, or have toxic effects on the microorganisms, thus affecting the growth of microorganisms and the efficiency of the downstream biorefinery process [39,[43][44][45][46].…”

Section: Introductionmentioning

confidence: 99%

Composition of Lignocellulose Hydrolysate in Different Biorefinery Strategies: Nutrients and Inhibitors

Wang,

Zhang,

Cui

et al. 2024

Molecules

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The hydrolysis and biotransformation of lignocellulose, i.e., biorefinery, can provide human beings with biofuels, bio-based chemicals, and materials, and is an important technology to solve the fossil energy crisis and promote global sustainable development. Biorefinery involves steps such as pretreatment, saccharification, and fermentation, and researchers have developed a variety of biorefinery strategies to optimize the process and reduce process costs in recent years. Lignocellulosic hydrolysates are platforms that connect the saccharification process and downstream fermentation. The hydrolysate composition is closely related to biomass raw materials, the pretreatment process, and the choice of biorefining strategies, and provides not only nutrients but also possible inhibitors for downstream fermentation. In this review, we summarized the effects of each stage of lignocellulosic biorefinery on nutrients and possible inhibitors, analyzed the huge differences in nutrient retention and inhibitor generation among various biorefinery strategies, and emphasized that all steps in lignocellulose biorefinery need to be considered comprehensively to achieve maximum nutrient retention and optimal control of inhibitors at low cost, to provide a reference for the development of biomass energy and chemicals.

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Transcriptome analysis reveals reasons for the low tolerance of Clostridium tyrobutyricum to furan derivatives

Cited by 8 publications

References 55 publications

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation

Composition of Lignocellulose Hydrolysate in Different Biorefinery Strategies: Nutrients and Inhibitors

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