Transcriptome analysis reveals pathogenicity and evolutionary history of the pathogenic oomycete Pythium insidiosum

Krajaejun, Theerapong; Garg, Gagan; Lowhnoo, Tassanee; Yingyong, Wanta; Khositnithikul, Rommanee; Tangphatsornruang, Sithichoke; Suriyaphol, Prapat; Ranganathan, Shoba; Sullivan, Thomas D.

doi:10.1016/j.funbio.2014.01.009

Cited by 26 publications

(27 citation statements)

References 55 publications

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“…litchii . High-throughput sequencing is a powerful and economical technology to uncover new genes and their involved biochemical pathways in non-model species[47–50]. Transcriptome analysis provide abundant sequence information and shed light on the molecular mechanism of pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Phytophthora litchii reveals pathogenicity arsenals and confirms taxonomic status

et al. 2017

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Litchi downy blight, caused by Peronophythora litchii, is one of the major diseases of litchi and has caused severe economic losses. P. litchii has the unique ability to produce downy mildew like sporangiophores under artificial culture. The pathogen had been placed in a new family Peronophytophthoraceae by some authors. In this study, the whole transcriptome of P. litchii from mycelia, sporangia, and zoospores was sequenced for the first time. A set of 23637 transcripts with an average length of 1284 bp was assembled. Using six open reading frame (ORF) predictors, 19267 representative ORFs were identified and were annotated by searching against several public databases. There were 4666 conserved gene families and various sets of lineage-specific genes among P. litchii and other four closely related oomycetes. In silico analyses revealed 490 pathogen-related proteins including 128 RXLR and 22 CRN effector candidates. Based on the phylogenetic analysis of 164 single copy orthologs from 22 species, it is validated that P. litchii is in the genus Phytophthora. Our work provides valuable data to elucidate the pathogenicity basis and ascertain the taxonomic status of P. litchii.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Phytophthora litchii reveals pathogenicity arsenals and confirms taxonomic status

et al. 2017

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“…Among the human pathogens, elicitins are present only in P. insidiosum and not in true fungi or other microorganisms (30)(31)(32)(33)(34)(35). One of the elicitins, ELI025, has been identified in P. insidiosum as a highly expressed protein on the cell surface (33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

“…One of the elicitins, ELI025, has been identified in P. insidiosum as a highly expressed protein on the cell surface (33)(34)(35). ELI025 has been successfully expressed and purified as a recombinant protein from the bacterium Escherichia coli (33).…”

Section: Discussionmentioning

confidence: 99%

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Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis

et al. 2016

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Pythiosis is an emerging and life-threatening infectious disease of humans and animals living in tropical and subtropical countries and is caused by the fungus-like organism Pythium insidiosum. Antifungals are ineffective against this pathogen. Most patients undergo surgical removal of the infected organ, and many die from advanced infections. Early and accurate diagnosis leads to prompt management and promotes better prognosis for affected patients. Immunohistochemical assays (IHCs) have been developed using rabbit antibodies raised against P. insidiosum crude extract, i.e., culture filtrate antigen (CFA), for the histodiagnosis of pythiosis, but cross-reactivity with pathogenic fungi compromises the diagnostic performance of the IHC. Therefore, there is a need to improve detection specificity. Recently, the elicitin protein, ELI025, was identified in P. insidiosum, but it was not identified in other human pathogens, including true fungi. The ELI025-encoding gene was successfully cloned and expressed as a recombinant protein in Escherichia coli. This study aims to develop a new IHC using the rabbit anti-ELI025 antibody (anti-ELI) and to compare its performance with the previously reported anti-CFA-based IHC. Thirty-eight P. insidiosum histological sections stained positive by anti-ELI-based and anti-CFA-based IHCs indicating 100% detection sensitivity for the two assays. The anti-ELI antibody stained negative for all 49 negative-control sections indicating 100% detection specificity. In contrast, the anti-CFA antibody stained positive for one of the 49 negative controls (a slide prepared from Fusarium-infected tissue) indicating 98% detection specificity. In conclusion, the anti-ELI based IHC is sensitive and specific for the histodiagnosis of pythiosis and is an improvement over the anti-CFA-based assay.

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“…Transcript mapping (11) and gene prediction by MAKER2 (12) revealed 14,962 open reading frames (ORFs), of which 3,579, 1,254, and 10,129 ORFs were predicted by both transcript mapping and gene prediction, only transcript mapping, or only gene prediction, respectively. A BLASTp search against the NCBI nonredundant protein database found significant matches ( E value, less than −6) in 89.7% of ORF-translated proteins.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of the Pathogenic Oomycete Pythium insidiosum Strain Pi-S, Isolated from a Patient with Pythiosis

et al. 2015

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Transcriptome analysis reveals pathogenicity and evolutionary history of the pathogenic oomycete Pythium insidiosum

Cited by 26 publications

References 55 publications

Transcriptome analysis of Phytophthora litchii reveals pathogenicity arsenals and confirms taxonomic status

Transcriptome analysis of Phytophthora litchii reveals pathogenicity arsenals and confirms taxonomic status

Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis

Draft Genome Sequence of the Pathogenic Oomycete Pythium insidiosum Strain Pi-S, Isolated from a Patient with Pythiosis

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