Transcriptome analysis reveals novel insights in air-breathing magur catfish (Clarias magur) in response to high environmental ammonia

Banerjee, Bodhisattwa; Koner, Debaprasad; Hasan, R.; Bhattacharya, Samir; Saha, Nirmalendu

doi:10.1016/j.gene.2019.04.009

Cited by 24 publications

(9 citation statements)

References 70 publications

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“…The clean reads were mapped to the C. sinensis reference genome and >77% could be unique mapped to the genome. This offered enough sequence coverage for RNA-seq analysis (Banerjee et al, 2019). Thus, the identified 2537 DEGs from the present study reflected the ammonia nitrogen response regulation credibly.…”

Section: Discussionsupporting

confidence: 53%

Combined analysis of mRNA–miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress

Shi²,

Jialing³

et al. 2021

Aquaculture Research

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The clam Cyclina sinensis is one of the most important economic bivalves in China and the clam industry has developed rapidly in recent years (Liu et al., 2002;Wei et al., 2020). C. sinensis is readily obtained in coastal intertidal zone of East Asia, particularly in coastal muddy tidal and estuaries. The clam possesses lots of advantages as one of the main aquaculture species of shellfish, including rapid growth,

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Section: Discussionsupporting

confidence: 53%

Combined analysis of mRNA–miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress

Shi²,

Jialing³

et al. 2021

Aquaculture Research

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“…Exposure to ZnO NPs also caused an increase in the activity of thioredoxin reductase (TrxR) in various tissues, which we speculated as an alternative to glutathione reductase (GR) for the maintenance of the GSH pool in this magur catfish. The transcriptome analysis of this magur catfish revealed the absence of any gene for the GR enzyme and the presence of the TrxR (txnrd1) gene (Banerjee et al 2019). Further, we also could not detect the GR activity in any of the tissues of magur catfish examined.…”

Section: Discussionmentioning

confidence: 64%

“…Similarly, the levels of mRNAs for cat, gst¸ gpx1, and trxnrd1 genes also increased significantly in different tissues of magur catfish during exposure to ZnO NPs. The whole transcriptome analysis, carried out under ammonia-induced oxidative stress in the liver and brain tissues of magur catfish, also demonstrated the possible induction of a set of antioxidant genes (Banerjee et al 2019). The increase in concentrations of mRNAs for different antioxidant genes was also supplemented by a significant rise in the abundance of various antioxidant enzyme proteins at variable levels in all the tissues examined during exposure to ZnO NPs.…”

Section: Discussionmentioning

confidence: 86%

Molecular characterization of superoxide dismutase and catalase genes, and the induction of antioxidant genes under the zinc oxide nanoparticle-induced oxidative stress in air-breathing magur catfish (Clarias magur)

Koner

Banerjee

Kumari

et al. 2021

Fish Physiol Biochem

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The deduced amino acid sequences from the complete cDNA coding sequences of three antioxidant enzyme genes (cmsod1, cmsod2, and cmcat) demonstrated that phylogenetically the magur catfish (Clarias magur) is very much close to other bony fishes with complete conservation of active site residues among piscine, amphibian and mammalian species. The three-dimensional structures of three antioxidant enzyme proteins are very much similar to mammalian counterparts, thereby suggesting the functional similarities of these enzymes. Exposure to ZnO NPs resulted in an oxidative stress as evidenced by an initial sharp rise of intracellular concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), but decreased gradually at later stages. The level of glutathione (GSH) also increased gradually in all the tissues examined after an initial decrease. Biochemical and gene expression analyses indicated that the magur catfish has the ability to defend the ZnO NP-induced oxidative stress by inducing the SOD/CAT enzyme system and also the GSH-related enzymes that are mediated through the activation of various antioxidant-related genes both at the transcriptional and translational levels in various tissues. Furthermore, it appeared that the stimulation of NO, as a consequence of induction nos2 gene, under NP-induced oxidative stress serves as a modulator to induce the SOD/CAT system in various tissues of magur catfish as an antioxidant strategy. Thus, it can be contemplated that the magur catfish possesses a very efficient antioxidant defensive mechanisms to defend against the oxidative stress during exposure to ZnO NPs into their natural environment, and also from related cellular damages.

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“…Although much attention has been paid to the effects of acute ammonia exposure on fish immunity, there are only limited studies on the effects of chronic or long-term ammonia stress and its potential molecular mechanism. In addition, some investigators have reported adverse effects of ammonia nitrogen on mRNA expression of TLRs through transcriptional analysis [ 16 , 19 , 20 , 21 , 22 ]. Consequently, we hypothesize that persistent ammonia exposure also could affect fish immunity, in which the TLR signaling pathway might play an important role.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Ammonia Nitrogen Exposure in Immune Defenses Present on Spleen and Head-Kidney of Wuchang Bream (Megalobrama amblycephala)

Guo

Chen²,

Ouyang

et al. 2022

IJMS

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Ammonia is one of the most important environmental factors in aquatic ecosystems. However, there are limited studies on the effects of chronic or long-term ammonia stress and its potential molecular mechanism in fish. This study aimed to investigate the immune response and molecular mechanisms in the spleen and head-kidney of fish following chronic ammonia exposure. Megalobrama amblycephala (9.98 ± 0.48 g) were exposed to different concentrations of total ammonia nitrogen (0–30 mg/L) for 30 days. Ammonia exposure caused significant increases in cortisol levels and decreases in lysozyme and complement 3/4 concentrations in the serum, indicating inhibitory effects of ammonia stress on innate immune responses. Ammonia exposure also induced concentration-dependent increases in ammonia concentrations in tissue, pathological damage and indexes of spleen and head-kidney. Additionally, the contents of immunoglobulin M (IgM), interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) as well as mRNA levels of toll-like receptors (TLRs)/Myeloid differentiation factor 88 (MyD88)-independent signaling molecules in the spleen and head-kidney were significantly downregulated after ammonia exposure. Our findings suggested that chronic ammonia exposure caused the suppression of innate and adaptive immune responses through downregulating TLR/MyD88-independent signaling. Adverse influences of chronic ammonia stress were more severe in the spleen than in the head-kidney.

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Transcriptome analysis reveals novel insights in air-breathing magur catfish (Clarias magur) in response to high environmental ammonia

Cited by 24 publications

References 70 publications

Combined analysis of mRNA–miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress

Combined analysis of mRNA–miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress

Molecular characterization of superoxide dismutase and catalase genes, and the induction of antioxidant genes under the zinc oxide nanoparticle-induced oxidative stress in air-breathing magur catfish (Clarias magur)

Evaluation of Ammonia Nitrogen Exposure in Immune Defenses Present on Spleen and Head-Kidney of Wuchang Bream (Megalobrama amblycephala)

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