Transcriptome analysis reveals genes potentially related to maize resistance to Rhizoctonia solani

Cao, Hongxiang; Yang, Zhangshuai; Song, Shaoxian; Xue, Mei; Liang, Guanyu; Li, Ning

doi:10.1016/j.plaphy.2022.10.029

Cited by 11 publications

(10 citation statements)

References 42 publications

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“…2 A and B and 3 A). This finding is consistent with the findings obtained for maize- R. solani interactions [ 23 ]. Furthermore, in a comparative transcriptional analysis of bls2 -mediated resistance to compatible Xoc strain (WT) and incompatible type III effectors deficiency Xoc strain (MT) interactions, there are 415 DEGs between WT and MT were identified at 12 hpi, while only 150 DEGs were found at 48 hpi [ 21 ].…”

Section: Discussionsupporting

confidence: 93%

“…RNA-seq analysis of the inoculation of two strains will be not only revealed the differential virulence involved genes, but also concentrated conserve DRs and reduced noise. These are also supported by recently published references for the inoculation of two fungi, R. solani YWK196 and YWK62, in maize [ 23 ]. Therefore, the DR genes were enriched by using comparative RNA-seq for inoculation with two closely related virulent strains.…”

Section: Discussionsupporting

confidence: 81%

“…Meng et al identified potential disease resistance genes by comparing the transcriptomes of powdery mildew-resistant and powdery mildew-susceptible cucumber materials [ 22 ]. Cao et al identified 388 potential key DR genes for banded leaf and sheath blight (BLSB) resistance by analyzing the transcriptomes of maize plants infected with a hypovirulent strain or a hypervirulent strain of Rhizoctonia solani for 3 or 5 days via transcriptomic deep sequencing [ 23 ]. Comparative transcriptomics analysis is used to reveal the expression patterns of conserved genes and identify homologs that perform the same function in different organisms, highlight genes that are critical to biological processes, and study gene evolution from the perspective of gene expression.…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains

Bi,

Yu,

Mao

et al. 2024

BMC Plant Biol

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Background Two-tiered plant immune responses involve cross-talk among defense-responsive (DR) genes involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), effector-triggered immunity (ETI) and effector-triggered susceptibility (ETS). Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzicola (Xoc) is an important bacterial disease that causes serious threats to rice yield and quality. Transcriptomic profiling provides an effective approach for the comprehensive and large-scale detection of DR genes that participate in the interactions between rice and Xoc. Results In this study, we used RNA-seq to analyze the differentially expressed genes (DEGs) in susceptible rice after inoculation with two naturally pathogenic Xoc strains, a hypervirulent strain, HGA4, and a relatively hypovirulent strain, RS105. First, bacterial growth curve and biomass quantification revealed that differential growth occurred beginning at 1 day post inoculation (dpi) and became more significant at 3 dpi. Additionally, we analyzed the DEGs at 12 h and 3 days post inoculation with two strains, representing the DR genes involved in the PTI and ETI/ETS responses, respectively. Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed on the common DEGs, which included 4380 upregulated and 4019 downregulated genes and 930 upregulated and 1383 downregulated genes identified for the two strains at 12 h post inoculation (hpi) and 3 dpi, respectively. Compared to those at 12 hpi, at 3 dpi the number of common DEGs decreased, while the degree of differential expression was intensified. In addition, more disease-related GO pathways were enriched, and more transcription activator-like effector (TALE) putative target genes were upregulated in plants inoculated with HGA4 than in those inoculated with RS105 at 3 dpi. Then, four DRs were randomly selected for the BLS resistance assay. We found that CDP3.10, LOC_Os11g03820, and OsDSR2 positively regulated rice resistance to Xoc, while OsSPX3 negatively regulated rice resistance. Conclusions By using an enrichment method for RNA-seq, we identified a group of DEGs related to the two stages of response to the Xoc strain, which included four functionally identified DR genes.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains

Bi,

Yu,

Mao

et al. 2024

BMC Plant Biol

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“…12,13 The pathogen generally causes necrotic lesions on leaves, sheaths and stems. 14 Infection of R. solani AG-3 causes black scurf, stem and stolon canker on potatoes, 1,15 forms sclerotia on sugar beet root, 16 and stem lesions of tomato plants that are irregular in shape, water-soaked, and brown,manifesting sunken appearance. 12 Nicotiana tabacum (N.tabacum) is an annual solanaceous crop that is widely planted in China.…”

Section: Introductionmentioning

confidence: 99%

“…R. solani AG‐3 was reported to infect crops such as soybean, 7 potato, 8,9 tobacco, 10 carrot, 11 tomato and onion 12,13 . The pathogen generally causes necrotic lesions on leaves, sheaths and stems 14 . Infection of R. solani AG‐3 causes black scurf, stem and stolon canker on potatoes, 1,15 forms sclerotia on sugar beet root, 16 and stem lesions of tomato plants that are irregular in shape, water‐soaked, and brown,manifesting sunken appearance 12 …”

Section: Introductionmentioning

confidence: 99%

RNA interference‐based exogenous double‐stranded RNAs confer resistance to Rhizoctonia solaniAG‐3 on Nicotiana tabacum

Wang,

Guo,

Guo

et al. 2024

Pest Management Science

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BACKGROUNDRhizoctonia solani Kühn is a pathogenic fungus causing tobacco target spot disease, and leads to great losses worldwide. At present, resistant varieties and effective control strategy on tobacco target spot disease are very limited. Host‐induced gene silencing (HIGS) as well as the exogenous dsRNA can be used to suppress disease progression, and reveal the function of crucial genes involved in the growth and pathogenesis of the fungus.RESULTSThe silencing of endoPGs or RPMK1 in host plants by TRV‐based HIGS resulted in a significant reduction in disease development in Nicotiana benthamiana. In vitro analysis validated that red fluorescence signals were consistently observed in the hyphae treated with Cy3‐fluorescein‐labeled dsRNA at 12, 24, 48 and 72 h postinoculation (hpi). Additionally, application of dsRNA‐endoPGs, dsRNA‐RPMK1 and dsRNA‐PGMK (fusion of partial endoPGs and RPMK1 sequences) effectively inhibited the hyphal growth of R. solani YC‐9 in vitro and suppressed disease progression in the leaves, and quantitative real‐time PCR confirmed that the application of dsRNAs significantly reduced the expression levels of endoPGs and RPMK1.CONCLUSIONThese results provide theoretical basis and new direction for RNAi approaches on the prevention and control of disease caused by R. solani. © 2024 Society of Chemical Industry.

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The F-box protein ZmFBL41 negatively regulates disease resistance to Rhizoctonia solani by degrading the abscisic acid synthase ZmNCED6 in maize

Yang,

Liang,

Liu

et al. 2024

Plant Cell Rep

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Transcriptome analysis reveals genes potentially related to maize resistance to Rhizoctonia solani

Cited by 11 publications

References 42 publications

Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains

Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains

RNA interference‐based exogenous double‐stranded RNAs confer resistance to Rhizoctonia solaniAG‐3 on Nicotiana tabacum

The F-box protein ZmFBL41 negatively regulates disease resistance to Rhizoctonia solani by degrading the abscisic acid synthase ZmNCED6 in maize

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