Transcriptome analysis reveals comprehensive responses to cadmium stress in maize inoculated with arbuscular mycorrhizal fungi

Gu, Longjiang; Zhao, Manli; Ge, Min; Zhu, Suwen; Cheng, Beijiu; Li, Xiaoyü

doi:10.1016/j.ecoenv.2019.109744

Cited by 31 publications

(19 citation statements)

References 41 publications

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“…ROS generation in plant cells can be induced by some environmental factors, such as cold ( Li et al, 2019 ), drought ( Zheng et al, 2020 ), heat ( Zhao et al, 2018 ), and cadmium stress ( Gu et al, 2019 ). ROS, as signal molecules, trigger signal transduction pathways in response to these abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

“…Deciphering the mechanism underlying maize seed germination in response to low-temperature stress can help improve low-temperature resistance. ROS generation in plant cells can be induced by some environmental factors, such as cold (Li et al, 2019), drought (Zheng et al, 2020), heat (Zhao et al, 2018), and cadmium stress (Gu et al, 2019). ROS, as signal molecules, trigger signal transduction pathways in response to these abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

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Maize Seed Germination Under Low-Temperature Stress Impacts Seedling Growth Under Normal Temperature by Modulating Photosynthesis and Antioxidant Metabolism

2022

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Spring maize is usually subjected to low-temperature stress during seed germination, which retards seedling growth later even under a suitable temperature. However, the mechanism underlying maize seed germination under low-temperature stress impacting seedling growth is still ambiguous. In this study, we used one low-temperature sensitive maize (SM) and one low-temperature resistance maize (RM) to investigate the mechanism. The results showed that the SM line had higher malondialdehyde content and lower total antioxidant capacity (TAC) and germination percentage than the RM line under low-temperature stress, indicating the vulnerability of SM line to low-temperature stress. Further transcriptome analysis revealed that seed germination under low-temperature stress caused the down-regulation of photosynthesis-related gene ontology terms in two lines. Moreover, the SM line displayed down-regulation of ribosome and superoxide dismutase (SOD) related genes, whereas genes involved in SOD and vitamin B6 were up-regulated in the RM line. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that photosynthesis and antioxidant metabolism-related pathways played essential roles in response to low-temperature stress during seed germination. The photosynthetic system displayed a higher degree of damage in the SM line. Both qRT-PCR and physiological characteristics experiments showed similar results with transcriptome data. Taken together, we propose a model for maize seed germination in response to low-temperature stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Maize Seed Germination Under Low-Temperature Stress Impacts Seedling Growth Under Normal Temperature by Modulating Photosynthesis and Antioxidant Metabolism

2022

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“…Meanwhile, calmodulin binding, metal ion binding, fungal-type cell wall, ATP biosynthetic process, heme binding, and membranes can be found in most C. geophilum strains after Cd treatment ( Figure 6 ). All of these regulatory pathways can be found in the arbuscular mycorrhizal fungi under heavy metal treatment [ 75 , 76 ], and show that some mechanisms are conserved in the heavy metal response, especially Cd treatment. Moreover, DEGs of nitrogen metabolism, valine, leucine and isoleucine biosynthesis, alanine, aspartate and glutamate metabolism, and 2-oxocarboxylic acid metabolism, methane metabolism, and oxidative phosphorylation pathways were indicated to play key roles in the Cd-induced ROS homeostasis ( Figure 8 ), which are inconsistent with high plants [ 77 , 78 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Physiological and Transcriptome Analysis Provide Insights into the Response of Cenococcum geophilum, an Ectomycorrhizal Fungus to Cadmium Stress

Shi

Yan

Yuan

et al. 2022

JoF

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Cadmium (Cd) displays strong toxicity, high mobility, and cannot be degraded, which poses a serious threat to the environment. Cenococcum geophilum (C. geophilum) is one of the most common ectomycorrhizal fungi (ECMF) in the natural environment. In this study, three Cd sensitive and three Cd tolerant strains of C. geophilum were used to analyze the physiological and molecular responses to Cd exposure. The results showed that Cd inhibited the growth of all strains of C. geophilum but had a less toxic effect on the tolerant strains, which may be correlated to a lower content of Cd and higher activity of antioxidant enzymes in the mycelia of tolerant strains. Comparative transcriptomic analysis was used to identify differentially expressed genes (DEGs) of four selected C. geophilum strains after 2 mg/L Cd treatment. The results showed that the defense response of C. geophilum strain to Cd may be closely related to the differential expression of functional genes involved in cell membrane ion transport, macromolecular compound metabolism, and redox pathways. The results were further confirmed by RT-qPCR analysis. Collectively, this study provides useful information for elucidation of the Cd tolerance mechanism of ECMF.

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“…ROS generation in plant cells can be induced by some environmental factors, such as cold (Li et al, 2019b), drought (Zheng et al, 2020), heat (Zhao et al, 2018), and cadmium stress (Gu et al, 2019). ROS, as signal molecules, trigger signal transduction pathways in response to these abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

Photosynthesis and antioxidant metabolism modulate the low-temperature resistance of seed germination in maize

Meng

Wen

Zhang

2021

Preprint

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Spring maize is usually subjected to low-temperature stress during seed germination, which retards seedling growth even if under a suitable temperature. However, the mechanism underlying maize seed germination under low-temperature stress modulating seedling growth after being transferred to normal temperature is still ambiguous. In this study, we used two maize inbred lines with different low-temperature resistance (SM and RM) to investigate the mechanism. The results showed that the SM line had higher lipid peroxidation and lower total antioxidant capacity and germination percentage than the RM line under low-temperature stress, which indicated that the SM line was more vulnerable to low-temperature stress. Further transcriptome analysis revealed that seed germination under low-temperature stress caused down-regulation of photosynthesis related gene ontology (GO) terms in two lines. Moreover, the SM line displayed down-regulation of ribosome and superoxide dismutase (SOD) related genes, whereas genes involved in SOD and vitamin B6 were up-regulated in the RM line. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that photosynthesis and antioxidant metabolism related pathways played important roles in seed germination in response to low-temperature stress, and the photosynthetic system displayed a higher damage degree in the SM line. Both qRT-PCR and physiological characteristics experiments showed similar results with transcriptome data. Taken together, we propose a model for maize seed germination in response to low-temperature stress.

show abstract

Transcriptome analysis reveals comprehensive responses to cadmium stress in maize inoculated with arbuscular mycorrhizal fungi

Cited by 31 publications

References 41 publications

Maize Seed Germination Under Low-Temperature Stress Impacts Seedling Growth Under Normal Temperature by Modulating Photosynthesis and Antioxidant Metabolism

Maize Seed Germination Under Low-Temperature Stress Impacts Seedling Growth Under Normal Temperature by Modulating Photosynthesis and Antioxidant Metabolism

Comparative Physiological and Transcriptome Analysis Provide Insights into the Response of Cenococcum geophilum, an Ectomycorrhizal Fungus to Cadmium Stress

Photosynthesis and antioxidant metabolism modulate the low-temperature resistance of seed germination in maize

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