Transcriptome Analysis Provides Insights into Lignin Biosynthesis in Styrax tonkinensis Branches

Abstract

Approximately 12% of China’s papermaking raw materials are derived from wood, while the majority are sourced from branches. Styrax tonkinensis is a more desirable species for pulpwood in subtropical ultra-short rotations, whose branches are prone to breakage. Lignin has a significant impact on wood quality and pulping yield, and the growth process influences lignin biosynthesis. To explore the lignin biosynthesis pathway in S. tonkinensis, we determined the lignin content in the current-year and biennial branches on 20 July, 20 September, and 20 October and analyzed the transcriptome sequencing results. It was concluded that the lignin content showed an increasing trend in the current-year branches (182.26, 206.17, and 213.47 mg/g, respectively), while that in the biennial branches showed a decrease in the samples taken in October, without significant difference (221.77, 264.43, and 261.83 mg/g, respectively). The transcriptome sequencing results showed that 91,513 unigenes were spliced with a total length of 92,961,618 bp. KEGG pathway analysis indicated that the upregulated DEGs were mainly enriched in the phenylpropanoid biosynthesis pathway. Our study suggested that CCoAOMT, COMT, peroxidase, and F5H may serve as key enzymes regulating lignin synthesis in branches of S. tonkinensis, thereby influencing the lignin content.