Transcriptome analysis provides insights into xylogenesis formation in Moso bamboo (Phyllostachys edulis) shoot

Zhang, Hui; Ying, Yibin; Wang, Jie; Zhao, Xiaoling; Zeng, Wei; Beahan, Cherie T.; He, Jun; Chen, Xiaoyang; Bacic, Antony; Song, Lili; Wu, Ai‐Min

doi:10.1038/s41598-018-21766-3

Cited by 33 publications

(30 citation statements)

References 69 publications

(75 reference statements)

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“…Peng et al [22] published the genome of bamboo shoots, provided information on potential candidate genes during the MBS fast-growth process, and speculated on the molecular basis of the physiological process. He et al [20] and Zhang et al [23] generated a transcriptome by taking advantage of the annotated Moso bamboo genome sequencing and delivered a molecular basis underlying this phenomenon of sequentially elongated internodes from base to top. However, little is known about the coordinated molecular mechanism of secondary wall thickening and lignification in MBS after harvest.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanism of Xylogenesis in Moso Bamboo (Phyllostachys edulis) Shoots during Cold Storage

Xuan

et al. 2018

Polymers

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A bamboo shoot is the immature stem of the woody grass and a nutritious and popular vegetable in East Asia. However, it undergoes a rapid xylogenesis process right after harvest, even being stored in a cold chamber. To investigate the molecular regulation mechanisms of xylogenesis in Moso bamboo (Phyllostachys edulis) shoots (MBSes) during cold storage, the measurement of cell wall polymers (cellulose, hemicellulose, and lignin) and related enzyme activities (phenylalanine ammonia lyase (PAL), cinnamyl alcohol dehydrogenase (CAD), peroxidase (POD), and xylan xylosyltransferase (XylT)) and transcriptomic analysis were performed during cold storage. It was noticed that cellulose and lignin contents increased, while hemicellulose content exhibited a downward trend. PAL, CAD, and POD activity presented an upward trend generally in MBS when stored at 4 °C for 16 days. XylT activity showed a descending trend during the stages of storage, but slightly increased during the 8th to 12th days after harvest at 4 °C. Transcriptomic analysis identified 72, 28, 44, and 31 functional unigenes encoding lignin, cellulose, xylan biosynthesis enzymes, and transcription factors (TFs), respectively. Many of these secondary cell wall (SCW)-related genes showed higher expression levels in the later period of cold storage. Quantitative RT-PCR analysis of the selected genes conformed to the expression pattern. Our study provides a comprehensive analysis of MBS secondary wall biosynthesis at the molecular level during the cold storage process. The results give insight into the xylogenesis process of this economically important vegetable and shed light on solving this problem of the post-harvest industry.

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Section: Introductionmentioning

confidence: 99%

Molecular Mechanism of Xylogenesis in Moso Bamboo (Phyllostachys edulis) Shoots during Cold Storage

Xuan

et al. 2018

Polymers

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“…In Arabidopsis , NAC TFs have mainly been characterized as master switches for secondary cell wall metabolism, rather than being the direct regulators of biosynthetic structural genes [ 39 ], with rare exceptions, such as AtVND7 and AtSND1 [ 40 , 41 ]. According to our previous report, some lignification-related TFs were chosen to explore the lignin biosynthesis of two types of bamboo shoots during storage at room temperature [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature

Zhang

et al. 2020

Plants

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Two types of bamboo shoots, high bamboo (Phyllostachys prominens) shoots (HBSes) and moso bamboo (Phyllostachys edulis) shoots (MBSes), underwent a fast post-harvest lignification process under room temperature storage. To explore the mechanism of lignification in two types of bamboo shoots after post-harvest during room temperature storage, the measurement of cell wall polymers (lignin and cellulose) and enzyme activities of phenylalanine ammonialyase (PAL) and peroxidase (POD), and relative expression of related transcription networks factors (TFs) were performed. The results suggested that the lignification process in HBSes is faster than that in MBSes because of incremental increase in lignin and cellulose contents within 6 days and the shorter shelf-life. Additionally, compared with the expression pattern of lignification-related TFs and correlation analysis of lignin and cellulose contents, MYB20, MYB43, MYB85 could function positively in the lignification process of two types of bamboo shoots. A negative regulator, KNAT7, could negatively regulate the lignin biosynthesis in two types of bamboo shoots. In addition, MYB63 could function positively in HBSes, and NST1 could function negatively in MBSes. Notably, MYB42 may function differently in the two types of bamboo shoots, that is, a positive regulator in HBSes, but a negative regulator in MBSes. Transcription networks provide a comprehensive analysis to explore the mechanism of lignification in two types of bamboo shoots after post-harvest during room temperature storage. These results suggest that the lignification of bamboo shoots was mainly due to the increased activity of POD, higher expression levels of MYB20, MYB43, MYB63, and MYB85 genes, and lower expression levels of KNAT7 and NST1 genes, and the lignification process of HBSes and MBSes had significant differences.

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“…Subcellular localization of PeUGDH4. Based on the fact that the expression level of PeUGDH4 was related to the lignification of bamboo shoots 26 , we considered it may be a key gene of PeUGDHs in the formation of cell wall. Thus, PeUGDH4 was selected to gain further transgene research.…”

Section: Phylogenetic Analysis Of the Peugdh Genes To Investigate Thmentioning

confidence: 99%

“…However, the specific expression mechanism of PeUGDHs remains to be determined. Interestingly, the Moso bambo MYB transcription factor has been identified to be involved in the regulation of cellulose and hemicellulose synthesis and are also related to the metabolism of lignin 26 . In the future, on behalf of the PeUGDH4 promoter sequence (we have cloned), key transcription factors binding to the cis-acting elements upstream of PeUGDH4 will be isolated and PeUGDH4 transcriptional regulation mechanism will be revealed.…”

Section: Overexpression Of Peugdh4 Caused Changes In Cell Wall Componmentioning

confidence: 99%

Genome-wide identification and characterization of UDP-glucose dehydrogenase family genes in moso bamboo and functional analysis of PeUGDH4 in hemicellulose synthesis

Yang

Kang

et al. 2020

Sci Rep

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Uridine diphosphate glucose dehydrogenases (UGDHs) are critical for synthesizing many nucleotide sugars and help promote the carbohydrate metabolism related to cell wall synthesis. In plants, UGDHs are encoded by a small gene family. Genome-wide analyses of these genes have been conducted in Glycine max and Arabidopsis thaliana, however, the UGDH gene family has not been comprehensively and systematically investigated in moso bamboo (Phyllostachys edulis), which is a special woody grass monocotyledonous species. In this study, we identified nine putative PeUGDH genes. Furthermore, analysis of gene duplication events and divergences revealed that the expansion of the PeUGDH family was mainly due to segmental and tandem duplications approximately 4.76-83.16 million years ago. An examination of tissue-specific PeUGDH expression indicated that more than 77% of the genes were predominantly expressed in the stem. Based on relative expression levels among PeUGDH members in different tissues in moso bamboo, PeUGDH4 was selected for detailed analysis. The results of subcellular localization indicated that PeUGDH4-GFP fusion proteins was observed to be localized in the cytoplasm. The ectopic overexpression of PeUGDH4 in Arabidopsis significantly increased the contents of hemicellulose and soluble sugar, suggesting that PeUGDH4 acts as a key enzyme involved in bamboo cell wall synthesis. The presence of a cell wall, which provides rigidity and flexibility, is one of the main characteristics that differentiates plant cells from animal cells. The cell wall, comprised mainly of complex polysaccharides (cellulose, hemicellulose, and pectin) and some structural proteins, is critical for plant growth 1-3. Additionally, UDP-glucose (UDP-Glc) is the chief form of activated sugar, representing a branch point of glucose metabolism 4 and a major substrate in many glycosylation reactions. For example, UDP-Glc is the substrate used by sucrose phosphate synthase to synthetize sucrose-6-phosphate in the cytosol. Moreover, in plastids, UDP-Glc is essential for the direct or indirect (via ADP-glucose) production of starch 5. Therefore, UDP-Glc is indispensable for the synthesis of sucrose, cellulose, and callose, which contribute to cell wall formation. The UDP-glucose dehydrogenases (UGDHs) are involved in the synthesis of matrix polysaccharides and can convert UDP-glucose into UDP-glucuronate (UDP-GlcA) and produce two NAD + molecules. Furthermore, UDP-GlcA is not only the intermediate pivot of polysaccharide metabolism, it is also an important glucuronic acid donor during cell wall polysaccharide synthesis. Previous studies revealed that UDP-GlcA continues to be glycosylated to form UDP-galacturonic acid, UDP-xylose, UDP-apiose, and other nucleoside sugars participating in the biosynthesis of hemicellulose and pectin, which represent over half of the cell wall biomass in Arabidopsis

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Transcriptome analysis provides insights into xylogenesis formation in Moso bamboo (Phyllostachys edulis) shoot

Cited by 33 publications

References 69 publications

Molecular Mechanism of Xylogenesis in Moso Bamboo (Phyllostachys edulis) Shoots during Cold Storage

Molecular Mechanism of Xylogenesis in Moso Bamboo (Phyllostachys edulis) Shoots during Cold Storage

Comparative Analysis of the Lignification Process of Two Bamboo Shoots Stored at Room Temperature

Genome-wide identification and characterization of UDP-glucose dehydrogenase family genes in moso bamboo and functional analysis of PeUGDH4 in hemicellulose synthesis

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