Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection

Wang, Yiya; Chen, Qiuyue; Liu, Zemin; Guo, Xiaoli; Du, Yanzhi; Yuan, Zhenjie; Guo, Miao; Li, Kang; Sun, Yuena; Jiang, Yunliang

doi:10.3389/fendo.2017.00317

Cited by 60 publications

(55 citation statements)

References 44 publications

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“…expression is helpful for elucidating mechanisms underlying follicle selection in chicken. By previous study we uncovered the role of Wnt4 in chicken follicle selection [4]. In this study, for the first time, we performed an integrated transcriptomic analysis on these SY follicles, identified an interaction network of SOWAHA, Integrated analysis indicated that the expression of SOWAHA was regulated by three miRNAs, i.e.…”

Section: Systemic Transcriptomic Analysis On Expression Differences Bmentioning

confidence: 85%

Integrated transcriptomic analysis on small yellow follicles reveals that sosondowah ankyrin repeat domain family member A inhibits chicken follicle selection

Zhong

Liu

Qiao³

et al. 2021

Anim Biosci

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Objective: Follicle selection is an important process in chicken egg laying. Among several small yellow (SY) follicles, the one exhibiting the highest expression of follicle stimulation hormone receptor (FSHR) will be selected to become hierarchal follicle. The role of lncRNA, miRNA and other non-coding RNA in chicken follicle selection is unclear. Methods: In this study, the whole transcriptome sequencing of SY follicles with different expression level of FSHR in Jining Bairi hens was performed, and the expression of 30 randomly selected mRNAs, lncRNAs and miRNAs was validated by qPCR. Preliminary studies and bioinformatics analysis were performed on the selected mRNA, lncRNA, miRNA and their target genes. The effect of identified gene was examined in the granulosa cells of chicken follicles. Results: Integrated transcriptomic analysis on chicken SY follicles differing in FSHR expression revealed 467 differentially expressed mRNA genes, 134 differentially expressed lncRNA genes and 34 differentially expressed miRNA genes, and sosondowah ankyrin repeat domain family member A (SOWAHA) was the common target gene of three miRNAs and one lncRNA. SOWAHA was mainly expressed in small white and SY follicles, and was affected by FSH treatment in the granulosa cells. Knockdown of SOWAHA inhibited the expression of Wnt4 and StAR in the granulosa cells of prehierarchal follicles, while stimulated Wnt4 in hierarchal follicles. Overexpression of SOWAHA increased the expression of Wnt4 in the granulosa cells of prehierarchal follicles, decreased StAR and CYP11A1 in the granulosa cells of hierarchal follicles and inhibited the proliferation of granulosa cells. A c c e p t e d A r t i c l e Conclusion: Integrated analysis on chicken small yellow follicle transcriptomes identified SOWAHA as a network gene that is affected by FSH in granulosa cells of ovarian follicles. SOWAHA affected the expression of genes involved in chicken follicle selection and inhibited the proliferation of granulosa cells, suggesting an inhibitory role in chicken follicle selection. .

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Section: Systemic Transcriptomic Analysis On Expression Differences Bmentioning

confidence: 85%

Integrated transcriptomic analysis on small yellow follicles reveals that sosondowah ankyrin repeat domain family member A inhibits chicken follicle selection

Zhong

Liu

Qiao³

et al. 2021

Anim Biosci

Self Cite

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“…The dynamic and highly regulated process requires the coordinated actions of a great number of genes, which is orderly orchestrated at the transcriptional and post-transcriptional levels [2]. While some of genes like Wnt family member 4 (Wnt4) [3], parathyroid hormone like hormone (PTHLH) [4], cytochrome P450 family 11 subfamily A member 1 (CYP11A1) [5] have been known are related to follicle development, new regulators continue to be uncovered. With the discovery of small RNAs that exert additional layers of control on gene expression, studies have begun looking at the roles these molecules play regulating cellular events in the ovary.…”

Section: Introductionmentioning

confidence: 99%

miR-458b-5p regulates ovarian granulosa cells proliferation through Wnt/β‐catenin signaling pathway by targeting catenin beta-1

et al. 2021

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Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA play an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear. Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-Catenin pathway. The Cell Counting Kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and Annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect the mRNA and protein expression levels. Results: We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells (TCs) of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and have no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule (CD44) and matrix metallopeptidase 7 (MMP7), which are the downstream effector of A c c e p t e d A r t i c l e CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation. Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development.

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“…Apparently, this process seems to be complex and highly coordinated, involving a number of divergent biological effects on the maturation of oocytes and differentiation and proliferation of granulosa and theca cells within the ovarian follicles [8]. In addition, the follicle-stimulating hormone (FSH) has been implicated in facilitating the recruitment of follicles [6,7,[9][10][11][12]. Similarly, follicle stimulating hormone receptors (FSHR) are reportedly expressed on the granulosa layer of developing follicles, and mRNA expression of these receptors (FSHR) changes with progressing follicular maturation [3,6,13].…”

Section: Introductionmentioning

confidence: 99%

Expression of FOXL2 and RSPO1 in Hen Ovarian Follicles and Implication of Exogenous Leptin in Modulating Their mRNA Expression in In Vitro Cultured Granulosa Cells

Niu

Qazi

et al. 2019

Animals

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In this study, using a laying hen model, we determined the expression of FOXL2 and RSPO1 in different central and peripheral tissue and ovarian follicles at different stages of development. At the same time, mRNA expression of both genes in granulosa and theca cells harvested from follicles at different stages of folliculogenesis was also evaluated. Finally, we assessed the effect of leptin treatment on expression of FOXL2 and RSPO1 in in vitro cultured granulosa cells harvested from 1–5 mm to F3–F1 follicles. Our RT-qPCR results revealed that a comparatively higher expression of FOXL2 and RSPO1 was observed in ovary, hypothalamus, and pituitary. Abundant mRNA expression of FOXL2 was observed in small prehierarchical follicles (1–1.9 and 2–2.9 mm follicles; p < 0.05), whereas mRNA expression of RSPO1 showed an increasing trend in large hierarchical follicles (F5–F1), and its abundant expression was observed in post-ovulatory follicles. FOXL2 mRNA expression was stable in granulosa cells harvested from 3–5 mm to F4 follicles, and exhibited a significantly higher expression in large hierarchical follicles. Conversely, relatively low mRNA expression of FOXL2 was observed in theca cells. RSPO1 mRNA expression was relatively lower in granulosa cells; however, theca cells exhibited a significantly higher mRNA expression of RSPO1 in F4 to F1 follicles. In the next experiment, we treated the in vitro cultured granulosa cells with different concentrations (1, 10, 100, and 1000 ng/mL) of exogenous leptin. Compared to the control group, a significant increase in the expression of FOXL2 was observed in groups treated with 1, 10, and 100 ng/mL leptin, whereas expression of RSPO1 was increased in all leptin-treated groups. When treated with 100 ng/mL leptin, FOXL2 and RSPO1 expression was upregulated in cultured granulosa cells harvested from both large hierarchical (F3–F1) and small prehierarchical follicles (1–5 mm). Based on these findings and evidence from mainstream literature, we envisage that FOXL2 and RSPO1 genes (in connection with hypothalamic-hypophysis axis) and leptin (via modulation of FOXL2 and RSPO1 expression) might have significant physiological roles, at least in part, in modulating the ovarian mechanisms, such as follicle development, selection, and steroidogenesis in laying hens.

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Transcriptome Analysis on Single Small Yellow Follicles Reveals That Wnt4 Is Involved in Chicken Follicle Selection

Cited by 60 publications

References 44 publications

Integrated transcriptomic analysis on small yellow follicles reveals that sosondowah ankyrin repeat domain family member A inhibits chicken follicle selection

Integrated transcriptomic analysis on small yellow follicles reveals that sosondowah ankyrin repeat domain family member A inhibits chicken follicle selection

miR-458b-5p regulates ovarian granulosa cells proliferation through Wnt/β‐catenin signaling pathway by targeting catenin beta-1

Expression of FOXL2 and RSPO1 in Hen Ovarian Follicles and Implication of Exogenous Leptin in Modulating Their mRNA Expression in In Vitro Cultured Granulosa Cells

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