Transcriptome analysis of turkey (Meleagris gallopavo) reproductive tract revealed key pathways regulating spermatogenesis and post-testicular sperm maturation

Słowińska, Mariola; Paukszto, Łukasz; Jastrzebski, J; Bukowska, Joanna; Kozłowski, Krzysztof; Jankowski, Jan; Ciereszko, Andrzej

doi:10.1016/j.psj.2020.07.031

Cited by 19 publications

(38 citation statements)

References 124 publications

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“…For chicken, Tingari and Lake (1972) reported three epithelial cell types responsible of sparmatophagy: the low cuboidal cells in the rete testis, the ciliated cell lining the efferent and connecting ducts in the epididymis and the non-ciliated type II cells in the vas deferens. On the other hand, Słowińska et al (2020) reported that apoptosis may be primarily involved in the regulation of post-testicular sperm maturation quality in turkey. Therefore, the induction of apoptosis in rooster spermatozoa that do not have proper chromatin condensation along the vas deferens should not be ruled out.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the induction of apoptosis in rooster spermatozoa that do not have proper chromatin condensation along the vas deferens should not be ruled out. A second possibility is that rearrangement of the rooster sperm chromatin continues in the vas deferens by phosphorylation of protamines as it happens in the testes (Słowińska et al 2020). Although it is assumed that chromatin condensation of mammalian spermatozoa is completed when they reach the cauda of the epididymis (which is well-developed in mammals), Bauer et al (2005) discovered that the spermatozoa of the eutherian mammal spinifex hopping mouse (Notomys alexis) spermatozoa do not finish the maturation process in the epididymis but undergo chromatin compaction in the vas deferens (Bauer et al 2005), probably due to the short size of the epididymis which results in a rapid epididymal transit; a shared characteristic with birds.…”

Section: Discussionmentioning

confidence: 99%

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Transit along the vas deferens results in a high percentage of filiform spermatozoa with compacted chromatin in the rooster (

Bernal

Behnamifar²,

Álvarez-Rodríguez³

et al. 2022

Reprod. Fertil. Dev.

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The present work aimed to evaluate the chromatin compaction of rooster spermatozoa along the male reproductive tract, and to study the vas deferens lining cells, potentially involved in sperm maturation. Chromomycin A3 (CMA3) was used to determine the chromatin compaction of spermatozoa from testis (T), proximal (including epididymis, V1), intermediate (V2) and distal (V3) vas deferens, and ejaculate (E). Six Birchen Leonesa roosters were used. E was obtained in vivo by dorso-ventral massage. V1, V2 and V3 sperm were obtained post mortem (six pairs of vasa deferentia), by flushing. T was obtained by washing the testes, cut in halves. The fixed cells were stained with CMA3 and propidium iodide for flow cytometry assessment. Results showed higher (P < 0.01) median fluorescence intensity (lower chromatin compaction) of T (693.8 ± 30.2) than V1 (546.3 ± 17.7), V2 (515.1 ± 12.1), V3 (517.6 ± 12.3) and E (491.4 ± 16.1). Regarding the percentage of intensely stained cells, T differs (P < 0.05) from V2, V3 and E, V1 differs (P < 0.05) from V3 and E, while V2, V3 and E do not differ. The histological analysis revealed secretory capacity of the vas deferens. Our findings specified that the transit though the vas deferens results in high percentage of compacted chromatin spermatozoa in E.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transit along the vas deferens results in a high percentage of filiform spermatozoa with compacted chromatin in the rooster (

Bernal

Behnamifar²,

Álvarez-Rodríguez³

et al. 2022

Reprod. Fertil. Dev.

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“…Ubiquitinated ZP1 proteins, bound with ZP3 to form the insoluble fiber of the IPVL, are the target protein of the sperm proteasome [ 2 ]. Sperm proteasomes have been identified, so far, in chicken and turkey spermatozoa after LC/MS and RNA-Seq analyses, respectively [ 45 , 46 ]. During sperm penetration of the IPVL, the sperm proteasome degrades ubiquitinated ZP1, and the fragment of ZP1, as well as intact ZP3, are released from the IPVL [ 2 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…Genomic DNA was removed from RNA samples using a DNase I, Amplification Grade kit (Invitrogen by Thermo Fisher Scientific, Carlsband, CA, USA). The evaluation of RNA quantity and quality was carried out according to the protocol of Słowińska et al [ 46 ]. First, the purity and concentration of isolated RNA were determined using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome and Proteome Analysis Revealed Key Pathways Regulating Final Stage of Oocyte Maturation of the Turkey (Meleagris gallopavo)

Słowińska

Paukszto

Pardyak

et al. 2021

IJMS

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In birds, the zona pellucida (ZP) matrix that surrounds the ovulated oocyte—called the inner perivitelline layer—is involved in sperm–zona interaction and successful fertilization. To identify the important genes and proteins connected with the final step of egg development, next-generation sequencing and two-dimensional electrophoresis, combined with mass spectrometry, were used for the analysis of mature oocytes at the F1 developmental stage. A total of 8161 genes and 228 proteins were annotated. Six subfamilies of genes, with codes ZP, ZP1–4, ZPD, and ZPAX, were identified, with the dominant expression of ZPD. The main expression site for ZP1 was the liver; however, granulosa cells may also participate in local ZP1 secretion. A ubiquitination system was identified in mature oocytes, where ZP1 was found to be the main ubiquitinated protein. Analysis of transcripts classified in estrogen receptor (ESR) signaling indicated the presence of ESR1 and ESR2, as well as a set of estrogen-dependent genes involved in both genomic and nongenomic mechanisms for the regulation of gene expression by estrogen. Oxidative phosphorylation was found to be a possible source of adenosine triphosphate, and the nuclear factor erythroid 2-related factor 2 signaling pathway could be involved in the response against oxidative stress. Oocyte–granulosa cell communication by tight, adherens, and gap junctions seems to be essential for the final step of oocyte maturation.

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“…Furthermore, placenta-associated genes, such as thrombospondin type-1 domain-containing protein 4 ( THSD4 ), pregnancy-associated glycoprotein 5 ( PAG5 ), PAG7 , and PAG10 , were abundantly transcribed in sperm, suggesting their possible role in implantation and placental development [ 198 ]. The proper organization of the centrosome of sperm, in the function of the sperm transcriptome tektin-1 ( TEKT1 ) is essential for oocyte genome activation leading to successful fertilization, and any defects in this process might lead to male infertility [ 199 ]. MAP7 , PTK2 , PLK1S1 , microtubule associated protein 7 ( MYH9 ), and protein kinase C zeta type ( PRKCZ ) are essential for spermatogenesis and sperm function [ 25 ].…”

Section: Sperm Transcriptome Assay (Gene Expression)mentioning

confidence: 99%

Semen evaluation: methodological advancements in sperm quality-specific fertility assessment

et al. 2021

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Assessment of male fertility is based on the evaluation of sperm. Semen evaluation measures various sperm quality parameters as fertility indicators. However, semen evaluation has limitations, and it requires the advancement and application of strict quality control methods to interpret the results. This article reviews the recent advances in evaluating various sperm-specific quality characteristics and methodologies, with the help of different assays to assess sperm-fertility status. Sperm evaluation methods that include conventional microscopic methods, computer-assisted sperm analyzers (CASA), and flow cytometric analysis, provide precise information related to sperm morphology and function. Moreover, profiling fertility-related biomarkers in sperm or seminal plasma can be helpful in predicting fertility. Identification of different sperm proteins and diagnosis of DNA damage has positively contributed to the existing pool of knowledge about sperm physiology and molecular anomalies associated with different infertility issues in males. Advances in methods and sperm-specific evaluation has subsequently resulted in a better understanding of sperm biology that has improved the diagnosis and clinical management of male factor infertility. Accurate sperm evaluation is of paramount importance in the application of artificial insemination and assisted reproductive technology. However, no single test can precisely determine fertility; the selection of an appropriate test or a set of tests and parameters is required to accurately determine the fertility of specific animal species. Therefore, a need to further calibrate the CASA and advance the gene expression tests is recommended for faster and field-level applications.

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Transcriptome analysis of turkey (Meleagris gallopavo) reproductive tract revealed key pathways regulating spermatogenesis and post-testicular sperm maturation

Cited by 19 publications

References 124 publications

Transit along the vas deferens results in a high percentage of filiform spermatozoa with compacted chromatin in the rooster (

Transit along the vas deferens results in a high percentage of filiform spermatozoa with compacted chromatin in the rooster (

Transcriptome and Proteome Analysis Revealed Key Pathways Regulating Final Stage of Oocyte Maturation of the Turkey (Meleagris gallopavo)

Semen evaluation: methodological advancements in sperm quality-specific fertility assessment

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