Transcriptome analysis of the secretion stress response of Bacillus subtilis

Hyyryläinen, Hanne‐Leena; Sarvas, Matti; Kontinen, Vesa P.

doi:10.1007/s00253-005-1898-1

Cited by 72 publications

(77 citation statements)

References 18 publications

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“…Previously, we showed that the overproduction of PspA greatly increased the secretion of homologous and heterologous proteins through the Tat pathway by S. lividans (25). A similar effect was described previously for E. coli PspA (7), and in B. subtilis, it was shown that liaH is dramatically upregulated under conditions of secretion stress (8).…”

Section: Vol 190 2008mentioning

confidence: 64%

Characterization of the Streptomyces lividans PspA Response

2008

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Phage shock protein (Psp) is induced by extracytoplasmic stress that may reduce the energy status of the cell. It is encoded in Escherichia coli by the phage shock protein regulon consisting of pspABCDE and by pspF and pspG. The phage shock protein system is highly conserved among a large number of gram-negative bacteria. However, many bacterial genomes contain only a pspA homologue but no homologues of the other genes of the Psp system. This conservation indicates that PspA alone might play an important role in these bacteria. In Streptomyces lividans, a soil-borne gram-positive bacterium, the phage shock protein system consists only of the pspA gene. In this report, we showed that pspA encodes a 28-kDa protein that is present in both the cytoplasmic and the membrane fractions of the S. lividans mycelium. We demonstrated that the pspA gene is strongly induced under stress conditions that attack membrane integrity and that it is essential for growth and survival under most of these conditions. The data reported here clearly show that PspA plays an important role in S. lividans under stress conditions despite the absence of other psp homologues, suggesting that PspA may be more important in most bacteria than previously thought.

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Section: Vol 190 2008mentioning

confidence: 64%

Characterization of the Streptomyces lividans PspA Response

2008

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“…The most-studied TCS related to the cell envelope stress response in Firmicutes is LiaFSR of B. subtilis, a system originally identified in a study of the response of B. subtilis to bacitracin (378). This system also responds to the presence of other antibiotics that interfere with the lipid II cycle (vancomycin and ramoplanin), to the action of detergents and organic solvents, and to other unspecific stimuli on the cell envelope (433)(434)(435)(436)(437)(438). LiaFSR is a three-component system, as a third protein, LiaF, acts as a negative regulator of LiaRS-dependent signal transduction (439).…”

Section: Mechanosensitive Channelsmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

511

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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“…The LiaRS TCS is also strongly induced by cell-wall active antibiotics such as vancomycin, ramoplanin, bacitracin, and nisin (6,28,29) as well as by the human antimicrobial peptide LL37 (31). LiaRS is weakly induced by the antibiotics fosfomycin and tunicamycin (29), by detergents, ethanol, phenol and organic solvents (29), and under conditions of alkaline shock (37) or secretion stress (19). Weak induction is also observed during normal growth conditions in rich medium at the onset of stationary phase (21).…”

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confidence: 99%

TheyydFGHIJOperon ofBacillus subtilisEncodes a Peptide That Induces the LiaRS Two-Component System

2007

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The Bacillus subtilis LiaRS two-component system (TCS) responds to perturbations of the cell envelope induced by lipid II-interacting antibiotics, such as vancomycin, ramoplanin, nisin, and bacitracin. Here, we characterize Tn7-generated mutations that induce the liaRS TCS. In addition to insertions in liaF, a known negative regulator of the LiaRS TCS, we identified two disruptions in the last two genes of the yydFGHIJ operon. This operon is predicted to encode a 49-amino-acid peptide (YydF), a modification enzyme (YydG), a membrane-embedded protease (YydH), and an ATP-binding cassette (ABC) transporter (YydIJ). Genome sequence comparisons suggest that the yydFGHIJ operon may have been acquired by horizontal transfer. Inactivation of the YydIJ transporter resulted in increased expression from the LiaR-dependent P liaI promoter only in the presence of the yydFGH genes. Cells harboring the complete yydFGHIJ operon induced LiaR activity in cocultured cells lacking either this transporter or the complete operon. These results suggest that this operon is involved in the synthesis and export of a modified peptide (YydF*) that elicits cell envelope stress sensed by the LiaRS TCS.

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Transcriptome analysis of the secretion stress response of Bacillus subtilis

Cited by 72 publications

References 18 publications

Characterization of the Streptomyces lividans PspA Response

Characterization of the Streptomyces lividans PspA Response

Stress Physiology of Lactic Acid Bacteria

TheyydFGHIJOperon ofBacillus subtilisEncodes a Peptide That Induces the LiaRS Two-Component System

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