Transcriptome Analysis of the Regulatory Mechanism of FoxO on Wing Dimorphism in the Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

Xu, Nan; Wei, Sheng-Fei; Xu, Haijun

doi:10.3390/insects12050413

Cited by 9 publications

(7 citation statements)

References 43 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, 2020; Xu et al. , 2021). An analogous phenotype was found in D. melanogaster , in which loss of FoxO suppressed the reduction in cell number, and overexpression of FoxO caused reduced cell number (Jünger et al.…”

Section: Discussionmentioning

confidence: 99%

FoxO is required for optimal fitness of the migratory brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

et al. 2023

Self Cite

View full text Add to dashboard Cite

The forkhead box O (FoxO) protein is the main transcriptional effector downstream of the insulin/insulin‐like signaling pathway and regulates many developmental and physiological processes. Holometabolous insects with loss‐of‐function mutations in FoxO exhibit phenotypes distinct from those of hemimetabolous insects in which RNA interference was used. Despite the functional importance of FoxO, whether hemimetabolous insects share an evolutionally conserved function of FoxO with holometabolous insects remains to be clarified. We used the clustered regularly interspaced short palindromic repeats/CRISPR‐associated 9 (CRISPR/Cas9) genome editing‐system to establish a homozygous FoxO‐null mutant (NlFoxO4E) of the wing‐dimorphic brown planthopper (BPH) Nilaparvata lugens, an economically important insect pest of rice fields. The phenotypes of NlFoxO4E mutants included extended nymphal duration, shortened lifespan, reduced reproduction, and decreased stress resistance. In addition, depletion of NlFoxO promoted cell proliferation in wing buds and led to 100% long‐winged morphs, in stark contrast to short‐winged wild‐type BPHs. These findings indicate that NlFoxO is highly functionally conserved with its counterpart in holometabolous insects, and is required for optimal fitness of N. lugens. The insights from FoxO studies may facilitate the identification of potential target genes for BPH control applications.

show abstract

Section: Discussionmentioning

confidence: 99%

FoxO is required for optimal fitness of the migratory brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, reports on how different cell types respond–either by producing resistance chemicals or activating other molecular processes –to BPH feeding are lacking. Therefore, we selected 48 h post-infestation time for this study based on previous results that show significant changes in gene expression occurring between 24–48 hours of BPH infestation of rice leaves ( Liu Y. et al., 2021 ; Xu et al., 2021a ; Xue et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Single-Cell RNA sequencing of leaf sheath cells reveals the mechanism of rice resistance to brown planthopper (Nilaparvata lugens)

Zha,

Li,

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

The brown planthopper (BPH) (Nilaparvata lugens) sucks rice sap causing leaves to turn yellow and wither, often leading to reduced or zero yields. Rice co-evolved to resist damage by BPH. However, the molecular mechanisms, including the cells and tissues, involved in the resistance are still rarely reported. Single-cell sequencing technology allows us to analyze different cell types involved in BPH resistance. Here, using single-cell sequencing technology, we compared the response offered by the leaf sheaths of the susceptible (TN1) and resistant (YHY15) rice varieties to BPH (48 hours after infestation). We found that the 14,699 and 16,237 cells (identified via transcriptomics) in TN1 and YHY15 could be annotated using cell-specific marker genes into nine cell-type clusters. The two rice varieties showed significant differences in cell types (such as mestome sheath cells, guard cells, mesophyll cells, xylem cells, bulliform cells, and phloem cells) in the rice resistance mechanism to BPH. Further analysis revealed that although mesophyll, xylem, and phloem cells are involved in the BPH resistance response, the molecular mechanism used by each cell type is different. Mesophyll cell may regulate the expression of genes related to vanillin, capsaicin, and ROS production, phloem cell may regulate the cell wall extension related genes, and xylem cell may be involved in BPH resistance response by controlling the expression of chitin and pectin related genes. Thus, rice resistance to BPH is a complicated process involving multiple insect resistance factors. The results presented here will significantly promote the investigation of the molecular mechanisms underlying the resistance of rice to insects and accelerate the breeding of insect-resistant rice varieties.

show abstract

“…DNA replication and cell cycle have rarely been focused on as regulatory mechanisms of density-dependent polyphenism [ 33 ]. A recent study [ 42 ] showed that the regulation of DNA replication and the cell cycle is involved in the density-dependent wing polyphenism of the planthopper Nilaparvata lugenes . Together with this study, our results emphasize the importance of DNA replication and the cell cycle as the regulatory mechanisms of density-dependent polyphenism.…”

Section: Discussionmentioning

confidence: 99%

Meta-Analysis of Transcriptomes in Insects Showing Density-Dependent Polyphenism

Toga

Yokoi

Bono

2022

Insects

View full text Add to dashboard Cite

With increasing public data, a statistical analysis approach called meta-analysis, which combines transcriptome results obtained from multiple studies, has succeeded in providing novel insights into targeted biological processes. Locusts and aphids are representative of insect groups that exhibit density-dependent plasticity. Although the physiological mechanisms underlying density-dependent polyphenism have been identified in aphids and locusts, the underlying molecular mechanisms remain largely unknown. In this study, we performed a meta-analysis of public transcriptomes to gain additional insights into the molecular underpinning of density-dependent plasticity. We collected RNA sequencing data of aphids and locusts from public databases and detected differentially expressed genes (DEGs) between crowded and isolated conditions. Gene set enrichment analysis was performed to reveal the characteristics of the DEGs. DNA replication (GO:0006260), DNA metabolic processes (GO:0006259), and mitotic cell cycle (GO:0000278) were enriched in response to crowded conditions. To date, these processes have scarcely been the focus of research. The importance of the oxidative stress response and neurological system modifications under isolated conditions has been highlighted. These biological processes, clarified by meta-analysis, are thought to play key roles in the regulation of density-dependent plasticity.

show abstract

Transcriptome Analysis of the Regulatory Mechanism of FoxO on Wing Dimorphism in the Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

Cited by 9 publications

References 43 publications

FoxO is required for optimal fitness of the migratory brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

FoxO is required for optimal fitness of the migratory brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

Single-Cell RNA sequencing of leaf sheath cells reveals the mechanism of rice resistance to brown planthopper (Nilaparvata lugens)

Meta-Analysis of Transcriptomes in Insects Showing Density-Dependent Polyphenism

Contact Info

Product

Resources

About