Transcriptome analysis of the post-larvae of giant freshwater prawn (Macrobrachium rosenbergii) after IAG gene knockdown with microRNA interference

Qian, Hongli; Knepper, M. A.; Feng, Jianbin; Guo, Ziqi; Gong, Jing; Chen, Huangen; Bai, Haotian; Qiu, Gao‐Feng

doi:10.1016/j.ygcen.2022.114054

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…IAG regulates male differentiation in decapods. A similar report indicates that the knocking down of MrIAG negatively regulates MrFem-1 in the post-larvae of M. rosenbergii [55]. Based on the function of EsIAG, these findings suggest that EsFem-1c regulates male sexual development [56].…”

Section: Discussionsupporting

confidence: 69%

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

Zhu

Feng

et al. 2023

Animals

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Feminization-1c (Fem-1c) is important for sex differentiation in the model organism Caenorhabditis elegans. In our previous study, the basic molecular characteristics of the Fem-1c gene (EsFem-1c) in Eriocheir sinensis (Henri Milne Edwards, 1854) were cloned to determine the relationship with sex differentiation. In this study, the genomic sequence of EsFem-1c contained five exons and four introns, with an exceptionally long 3′UTR sequence. The qRT-PCR results of EsFem-1c demonstrated lower tissue expression in the androgenic gland of the intersex crab than the normal male crab, implying that EsFem-1c plays a role in crab AG development. RNA interference experiments and morphological observations of juvenile and mature crabs indicated that EsFem-1c influences sexual development in E. sinensis. A dual-luciferase reporter assay disclosed that tcf-miR-315-5p effectively inhibits the translation of the EsFem-1c gene, influencing male development. An intriguing finding was that miRNA tcf-miR-307 could increase EsFem-1c expression by binding to the alternative splicing region with a length of 248 bp (ASR-248) in the 3′UTR sequence. The present research contributes to a better understanding of the molecular regulation mechanism of EsFem-1c and provides a resource for future studies of the miRNA-mediated regulation of sexual development and regulation in E. sinensis.

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Section: Discussionsupporting

confidence: 69%

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

Zhu

Feng

et al. 2023

Animals

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“…Lack of effective tools has become a bottleneck for studying gene function in non-model organisms. RNA interference can significantly reduce the expression of target genes both in vivo and in vitro, and it is widely employed for loss-of-function studies in aquaculture animals 23,[32][33][34] . RNAi can be induced in organicism using siRNAs, dsRNAs and shRNAs [35][36][37] .…”

Section: Discussionmentioning

confidence: 99%

“…In the Chinese mitten crab (Eriocheir sinensis), injecting long dmrt-like dsRNA for one month led to the atrophy www.nature.com/scientificreports/ of seminiferous tubules and fewer male germ cells in the seminiferous tubules 47 . In the prawn (Macrobrachium rosenbergii), silencing the insulin-like androgenic gland hormone gene (IAGs) through a long-term in vivo injection of long dsRNAs resulted in complete and functional sex reversal of male freshwater prawns 34 . In future dsRNA-based RNAi knockdown experiments, increasing the number of injections and extending the duration of the injection experiment should be taken into full consideration.…”

Section: Discussionmentioning

confidence: 99%

A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas

Zhang,

Sun,

et al. 2023

Sci Rep

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The signal recognition particle (SRP) system delivers approximately 30% of the proteome to the endoplasmic reticulum (ER) membrane. SRP receptor alpha (SRα) binds to SRP for targeting nascent secreted proteins to the ER membrane in eukaryotic cells. In this study, the SRα homologous gene was identified in the sea cucumber, Apostichopus japonicus (AjSRα). AjSRα codes for 641 amino acids and has 54.94% identity with its mammalian homologs. Like mammalian SRα, it is expected to contain the SRP-alpha N domain, SRP54_N domain, and SRP54 domain. In addition, AjSRα is ubiquitously expressed in adult tissues and exhibits a sexually dimorphic expression pattern, with significantly higher expression in ovaries compared to testes. As a maternal factor, AjSRα can be continuously detected during embryonic development. Importantly, we first attempted to investigate its function by using lentiviral vectors for delivering SRα gene-specific shRNA, and we revealed that lentiviral vectors do not induce an upregulation of immune-related enzymes in sea cucumbers. However, compared to the dsRNA-based RNA interference (RNAi) method, lentivirus-mediated RNAi caused dynamic changes in gene expression at a later time. This study supplied the technical support for studying the functional mechanism of SRα in sea cucumbers.

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“…In M. rosenbergii, MrDsx expression was significantly induced in the testis and AG following eyestalk ablation, and gene knockdown of MrDsx resulted in a significant decrease in MrIAG transcript levels in the AG, suggesting that eyestalk elements negatively control MrDsx, which regulates the activation of MrIAG [77]. As part of the extensive research aimed at elucidating the IAG signaling cascade in decapod species, IAG loss-of-function studies have played a significant role in the understanding of the IAG downstream factors [78][79][80][81][82][83]. Still, in most of the studies on candidates upstream of the IAG-switch, it seems that the intervention (usually RNAi) is too late to effectively influence sexual differentiation.…”

Section: Controlling Elements Of the Iag-switchmentioning

confidence: 99%

Monosex Populations of the Giant Freshwater Prawn Macrobrachium rosenbergii—From a Pre-Molecular Start to the Next Generation Era

Wahl,

Levy,

Ventura

et al. 2023

IJMS

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Sexual manipulation in the giant freshwater prawn Macrobrachium rosenbergii has proven successful in generating monosex (both all-male and all-female) populations for aquaculture using a crustacean-specific endocrine gland, the androgenic gland (AG), which serves as a key masculinizing factor by producing and secreting an insulin-like AG hormone (IAG). Here, we provide a summary of the advancements from the discovery of the AG and IAG in decapods through to the development of monosex populations in M. rosenbergii. We discuss the broader sexual development pathway, which is highly divergent across decapods, and provide our future perspective on the utility of novel genetic and genomic tools in promoting refined approaches towards monosex biotechnology. Finally, the future potential benefits of deploying monosex prawn populations for environmental management are discussed.

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Transcriptome analysis of the post-larvae of giant freshwater prawn (Macrobrachium rosenbergii) after IAG gene knockdown with microRNA interference

Cited by 5 publications

References 38 publications

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas

Monosex Populations of the Giant Freshwater Prawn Macrobrachium rosenbergii—From a Pre-Molecular Start to the Next Generation Era

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