Transcriptome analysis of the pheromone glands in Noorda blitealis reveals a novel AOX group of the superfamily Pyraloidea

Zhang, Zu-Bing; Yin, Ning-Na; Ji-ming, Long; Zhang, Yongke; Liu, Nai-Yong; Zhu, Jia‐Ying

doi:10.1016/j.aspen.2020.11.007

Cited by 2 publications

(9 citation statements)

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“… Following this classification system of AOXs previously described in Lepidoptera ( Zhang et al, 2021 ), six AyunAOXs clustered into six subfamilies, in which AyunAOX6 harbored two relatives. Candidate AyunAOXs and XDHs newly identified in this study were presented in red.…”

Section: Resultsmentioning

confidence: 84%

“…Based on a previous classification system of AOXs in Lepidoptera ( Zhang et al, 2021 ), AyunAOXs clustered in five orthologous clades: AOX2–AOX6. Members of an AOX2 clade functioned in the degradation of aldehyde compounds contained AyunAOX2, which shared an average of 66.87% identity with other members in this clade ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In moths, AOXs could be distinguished into six groups with a recently proposed clade of AOX6 in pyralid species ( Zhang et al, 2021 ). Based on the phylogenetic analysis of lepidopteran AOXs, it was found that the A. yunnanensis transcriptome did not comprise a member homologous to AOX1.…”

Section: Discussionmentioning

confidence: 99%

“…, ACCs, FASs, FADs, FARs, aldehyde reductases (ARs), aldehyde dehydrogenases (ALDHs), AOs/ADHs, ATFs, fatty acid transport proteins (FATPs) and AOXs), from Noorda blitealis ( Zhang et al, 2021 ), two Spodoptera species ( S. exigua and S. litura ) ( Zhang et al, 2015 ; Zhang et al, 2017 ), three Helicoverpa species ( H. armigera , H. assulta and H. zea ) ( Li et al, 2015 ; Dou et al, 2019a ) and Plutella xylostella ( He et al, 2017 ). For the identification of AOXs, except for the above six moths, the corresponding protein sequences from three additional moths, Amyelois transitella , Chilo suppressalis and Ostrinia furnacalis , were included ( Zhang et al, 2021 ). To avoid missing any pheromone biosynthesis and degradation related genes, newly identified genes were used as queries to screen the transcriptome.…”

Section: Methodsmentioning

confidence: 99%

“…Except for the biosynthesis of sex pheromones, the pheromone degradation also requires a variety of enzymes such as aldehyde oxidases (AOXs). In Lepidoptera, the AOX gene family comprises six conserved groups (AOX1–AOX6), in which a novel clade (AOX6) was more recently proposed ( Xu & Liao, 2017 ; Zhang et al, 2021 ). Functional experiments have demonstrated that AOXs enriched in moth antennae could degrade aldehyde compounds into carboxylic acids, including aldehyde-containing sex pheromones, plant volatiles and insecticides ( Choo et al, 2013 ; Wang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis identifies candidate genes in the biosynthetic pathway of sex pheromones from a zygaenid moth, Achelura yunnanensis (Lepidoptera: Zygaenidae)

Nuo

Yang

et al. 2021

PeerJ

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In most moth species, sex pheromones responsible for mating and communication of both sexes are primarily produced by the pheromone glands (PGs) of female moths. Although the PG transcriptomes and pheromone production related genes from 24 moth species have been characterized, studies on the related information remain unknown in the Zygaenidae family. Here, we sequenced the PG transcriptome of a zygaenid moth, Achelura yunnanensis. Such the sequencing resulted in the yields of 47,632,610 clean reads that were assembled into 54,297 unigenes, coupled with RNA sequencing data from 12 other tissues. Based on the transcriptome, a total of 191 genes encoding pheromone biosynthesis and degradation enzymes were identified, 161 of which were predicted to have full-length sequences. A comparative analysis among 24 moth species of nine families indicated that the numbers of the genes were variable, ranging from 14 in two Grapholita species to 191 in A. yunnanensis. Phylogenetic analysis in parallel with the expression data highlighted some key genes, including three △9 and four △11 desaturases, four fatty acyl-CoA reductases (FARs) clustering in the pgFAR clade, and three significantly antennae-enriched aldehyde oxidases. An extensive tissue- and sex- expression profile revealed a broad distribution of the genes, in which 128 relatives were detected in the PGs and 127 in the antennae. This study reports, for the first time, the gene repertoires associated with the pheromone production in Zygaenidae, and provides a valuable resource for exploring putative roles of the PG-enriched genes in A. yunnanensis.

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Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis identifies candidate genes in the biosynthetic pathway of sex pheromones from a zygaenid moth, Achelura yunnanensis (Lepidoptera: Zygaenidae)

Nuo

Yang

et al. 2021

PeerJ

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Genome-Wide Identification and Expression Profiling of Candidate Sex Pheromone Biosynthesis Genes in the Fall Armyworm (Spodoptera frugiperda)

Kang

Zhang

et al. 2022

Insects

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Spodoptera frugiperda is an agricultural pest causing substantial damage and losses to commercial crops. Sex pheromones are critical for successful mating in Lepidoptera and have been used for monitoring and control of many pest species. The sex pheromone of S. frugiperda is known, but the genes involved in its biosynthesis have not been identified. We systematically studied 99 candidate sex pheromone genes in the genome of S. frugiperda including 1 acetyl-CoA carboxylase (ACC), 11 fatty acid synthases (FASs), 17 desaturases (DESs), 4 fatty acid transport proteins (FATPs), 29 fatty acyl-CoA reductases (FARs), 17 acetyl-CoA acetyltransferases (ACTs), 5 acyl-CoA dehydrogenase (ACDs), 3 enoyl-CoA hydratases (ECHs), 3 hydroxyacyl-CoA dehydrogenases (HCDs), 6 ethyl-CoA thiolases (KCTs), and 3 acyl-CoA-binding proteins (ACBPs). Based on the comparative transcriptome results, we found 22 candidate sex pheromone biosynthesis genes predominately expressed in pheromone glands (PGs) than abdomens without PGs including SfruFAS4, SfruFATP3, SfruACD5, SfruKCT3, SfruDES2, SfruDES5, SfruDES11, SfruDES13, SfruFAR1, SfruFAR2, SfruFAR3, SfruFAR6, SfruFAR7, SfruFAR8, SfruFAR9, SfruFAR10, SfruFAR11, SfruFAR14, SfruFAR16, SfruFAR29, SfruACT6, and SfruACT10. A combination of phylogenetic and tissue-specific transcriptomic analyses indicated that SfruDES5, SfruDES11, SfruFAR2, SfruFAR3, and SfruFAR9 may be key genes involved in the sex pheromone synthesis of S. frugiperda. Our results could provide a theoretical basis for understanding the molecular mechanisms of sex pheromone biosynthesis in S. frugiperda, and also provide new targets for developing novel pest control methods based on disrupting sexual communication.

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Transcriptome analysis of the pheromone glands in Noorda blitealis reveals a novel AOX group of the superfamily Pyraloidea

Cited by 2 publications

References 50 publications

Transcriptome analysis identifies candidate genes in the biosynthetic pathway of sex pheromones from a zygaenid moth, Achelura yunnanensis (Lepidoptera: Zygaenidae)

Transcriptome analysis identifies candidate genes in the biosynthetic pathway of sex pheromones from a zygaenid moth, Achelura yunnanensis (Lepidoptera: Zygaenidae)

Genome-Wide Identification and Expression Profiling of Candidate Sex Pheromone Biosynthesis Genes in the Fall Armyworm (Spodoptera frugiperda)

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