Transcriptome analysis of the entomopathogenic fungus Beauveria bassiana grown on cuticular extracts of the coffee berry borer (Hypothenemus hampei)

Mantilla, Javier Guillermo; Galeano, Narmer F.; Gaitán, Álvaro; Cristancho, Marco; Keyhani, Nemat O.; Góngora, Carmenza E.

doi:10.1099/mic.0.051664-0

Cited by 38 publications

(16 citation statements)

References 44 publications

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“…These cells are able to evade immune cells and/ or survive within phagocytic cells, a phenomenon paralleling several microbial animal pathogens that are capable of surviving within host macrophages (Bidochka et al 2010;Kurtti and Keyhani 2008). Programs of gene expression in response to cuticle and cuticular components ultimately lead to production of the factors needed for successful mycosis to occur (Chantasingh et al 2013;Cho et al 2006aCho et al , 2006bMantilla et al 2012). And, although more of these genes are being identified, additional research is needed to better understand the temporal expression of these factors that undoubtedly contribute to different stages of the infection process.…”

Section: Conclusion and Future Directions: Stress And Virulencementioning

confidence: 97%

Stress response signaling and virulence: insights from entomopathogenic fungi

2014

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The Ascomycete fungal insect pathogens, Beauveria and Metarhizium spp. have emerged as model systems with which to probe diverse aspects of fungal growth, stress response, and pathogenesis. Due to the availability of genomic resources and the development of robust methods for genetic manipulation, the last 5 years have witnessed a rapid increase in the molecular characterization of genes and their pathways involved in stress response and signal transduction in these fungi. These studies have been performed mainly via characterization of gene deletion/knockout mutants and have included the targeting of general proteins involved in stress response and/or virulence, e.g. catalases, superoxide dismutases, and osmolyte balance maintenance enzymes, membrane proteins and signaling pathways including GPI anchored proteins and G-protein coupled membrane receptors, MAPK pathways, e.g. (i) the pheromone/nutrient sensing, Fus3/Kss1, (ii) the cell wall integrity, Mpk1, and (iii) the high osmolarity, Hog1, the PKA/adenyl cyclase pathway, and various downstream transcription factors, e.g. Msn2, CreA and Pac1. Here, we will discuss current research that strongly suggests extensive underlying contributions of these biochemical and signaling pathways to both abiotic stress response and virulence.

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Section: Conclusion and Future Directions: Stress And Virulencementioning

confidence: 97%

Stress response signaling and virulence: insights from entomopathogenic fungi

2014

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“…(Cordyceps) bassiana, M. robertsii, M. anisopliae, and the narrower host specific locust pathogen, Metarhizium acridum (Gao et al 2011;Pattemore et al 2014;Xiao et al 2012;Zheng et al 2013). In addition, extensive transcriptomics data including of these fungi growing on insect cuticles are available (Cho et al 2006a;Cho et al 2006b;Freimoser et al 2003;Mantilla et al 2012). Comparative genomic analyses, particularly between B. bassiana and M. roberstii, indicate that although similarities regarding insect virulence exist, significant aspects of the molecular and biochemical mechanisms and even cellular structures employed during the invasion process are dissimilar.…”

Section: Insect and Plant Interactionsmentioning

confidence: 98%

Improving mycoinsecticides for insect biological control

Ortiz‐Urquiza

Luo

Keyhani

2014

Appl Microbiol Biotechnol

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The desire for decreased reliance on chemical pesticides continues to fuel interest in alternative means for pest control including the use of naturally occurring microbial insect pathogens. Insects, as vectors of disease causing agents or as agricultural pests, are responsible for millions of deaths and significant economic losses worldwide, placing stresses on productivity (GDP) and human health and welfare. In addition, alterations in climate change are likely to affect insect ranges, expanding their access to previously constrained geographic areas, a potentially worrisome outcome. Metarhizium anisopliae and Beauveria bassiana, two cosmopolitan fungal pathogens of insects found in almost all ecosystems, are the most commonly applied mycoinsecticides for a variety of insect control purposes. The availability of the complete genomes for both organisms coupled to robust technologies for their transformation has led to several advances in engineering these fungi for greater efficacy and/or utility in pest control applications. Here, we will provide an overview of the fungal-insect and fungal-plant interactions that occur and highlight recent advances in the genetic engineering of these fungi. The latter work has resulted in the development of strains displaying (1) increased resistance to abiotic stress, (2) increased cuticular targeting and degradation, (3) increased virulence via expression of insecticidal protein/peptide toxins, (4) the ability to block transmission of disease causing agents, and (5) the ability to target specific insect hosts, decrease host fecundity, and/or alter insect behaviors.

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“…In this regard, the high expression levels of both catA and catB genes detected in alkane-grown B. bassiana, and the correlation between higher catalase activity and faster germination reported in alkane-grown B. bassiana (Pedrini et al 2007) and I. fumosorosea (Ali et al 2013) compared to the controls, suggest an important function for catalases in H 2 O 2 consumption by fungi, and hence facilitating conidia germination on these carbon sources. In B. bassiana s.l., a transcriptome analysis of fungi grown on insect cuticle showed that peroxidases and catalases are up-regulated in cuticle-germinated conidia compared with cuticle-grown mycelia (Mantilla et al 2012). Transgenic strains of M. anisopliae s.l.…”

Section: Catalasesmentioning

confidence: 99%

Oxidative stress in entomopathogenic fungi grown on insect-like hydrocarbons

2014

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Entomopathogenic fungi mostly attack their insect hosts by penetration through the cuticle. The outermost insect surface is covered by a lipid-rich layer, usually composed of very long chain hydrocarbons. These fungi are apt to grow on straight chain hydrocarbons (alkanes) as the sole carbon source. Insect-like hydrocarbons are first hydroxylated by a microsomal P450 monooxygenase system, and then fully catabolized by peroxisomal β-oxidation reactions in Beauveria bassiana. In this review, we will discuss lipid metabolism adaptations in alkane-grown fungi, and how an oxidative stress scenario is established under these conditions. Fungi have to pay a high cost for hydrocarbon utilization; high levels of reactive oxygen species are produced and a concomitant antioxidant response is triggered in fungal cells to cope with this drawback.

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Transcriptome analysis of the entomopathogenic fungus Beauveria bassiana grown on cuticular extracts of the coffee berry borer (Hypothenemus hampei)

Cited by 38 publications

References 44 publications

Stress response signaling and virulence: insights from entomopathogenic fungi

Stress response signaling and virulence: insights from entomopathogenic fungi

Improving mycoinsecticides for insect biological control

Oxidative stress in entomopathogenic fungi grown on insect-like hydrocarbons

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