Transcriptome analysis of the brain of the Chinese mitten crab, Eriocheir sinensis, for neuropeptide abundance profiles during ovarian development

Li, Xue; Knepper, M. A.; Liu, Zhiqiang; Feng, Jianbin; Ye, Baoqing; Qiu, Gao‐Feng

doi:10.1016/j.anireprosci.2018.12.010

Cited by 15 publications

(9 citation statements)

References 29 publications

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“…In some ILPs, the C chain is not completely removed, producing a polypeptide, in which part of the C chain is retained (77). ILPs have been identified in decapod crustacean transcriptomes (28,32,33,38,83,(110)(111)(112)(113)(114). One of the best characterized ILP is the insulin-like androgenic gland hormone (IAG); it is expressed primarily in the androgenic gland and determines adult male characteristics (42,83,108,109,115,116).…”

Section: Insulin-like Peptide Receptor Signalingmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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A pair of Y-organs (YOs) are the molting glands of decapod crustaceans. They synthesize and secrete steroid molting hormones (ecdysteroids) and their activity is controlled by external and internal signals. The YO transitions through four physiological states over the molt cycle, which are mediated by molt-inhibiting hormone (MIH; basal state), mechanistic Target of Rapamycin Complex 1 (mTORC1; activated state), Transforming Growth Factor-β (TGFβ)/Activin (committed state), and ecdysteroid (repressed state) signaling pathways. MIH, produced in the eyestalk X-organ/sinus gland complex, inhibits the synthesis of ecdysteroids. A model for MIH signaling is organized into a cAMP/Ca2+-dependent triggering phase and a nitric oxide/cGMP-dependent summation phase, which maintains the YO in the basal state during intermolt. A reduction in MIH release triggers YO activation, which requires mTORC1-dependent protein synthesis, followed by mTORC1-dependent gene expression. TGFβ/Activin signaling is required for YO commitment in mid-premolt. The YO transcriptome has 878 unique contigs assigned to 23 KEGG signaling pathways, 478 of which are differentially expressed over the molt cycle. Ninety-nine contigs encode G protein-coupled receptors (GPCRs), 65 of which bind a variety of neuropeptides and biogenic amines. Among these are putative receptors for MIH/crustacean hyperglycemic hormone neuropeptides, corazonin, relaxin, serotonin, octopamine, dopamine, allatostatins, Bursicon, ecdysis-triggering hormone (ETH), CCHamide, FMRFamide, and proctolin. Contigs encoding receptor tyrosine kinase insulin-like receptor, epidermal growth factor (EGF) receptor, and fibroblast growth factor (FGF) receptor and ligands EGF and FGF suggest that the YO is positively regulated by insulin-like peptides and growth factors. Future research should focus on the interactions of signaling pathways that integrate physiological status with environmental cues for molt control.

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Section: Insulin-like Peptide Receptor Signalingmentioning

confidence: 99%

Signaling Pathways That Regulate the Crustacean Molting Gland

Mykles

2021

Front. Endocrinol.

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“…A putative EH transcript was identified in the testis transcriptome database of P. trituberculatus ( Supplemental Information 7 ). In insects, FLRFamide is reported in endocrine and paracrine cells of the midgut ( Kingan et al., 1997 ), however, only a few decapod crustacean species have been reported to harbor these peptides in CNS ( Bao et al., 2015 ; Liu et al., 2019c ). Analysis showed that the peptides were expressed in different tissue among diverse species.…”

Section: Resultsmentioning

confidence: 99%

Identification and characterization of expression profiles of neuropeptides and their GPCRs in the swimming crab, Portunus trituberculatus

Wang

et al. 2021

PeerJ

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Neuropeptides and their G protein-coupled receptors (GPCRs) regulate multiple physiological processes. Currently, little is known about the identity of native neuropeptides and their receptors in Portunus trituberculatus. This study employed RNA-sequencing and reverse transcription-polymerase chain reaction (RT-PCR) techniques to identify neuropeptides and their receptors that might be involved in regulation of reproductive processes of P. trituberculatus. In the central nervous system transcriptome data, 47 neuropeptide transcripts were identified. In further analyses, the tissue expression profile of 32 putative neuropeptide-encoding transcripts was estimated. Results showed that the 32 transcripts were expressed in the central nervous system and 23 of them were expressed in the ovary. A total of 47 GPCR-encoding transcripts belonging to two classes were identified, including 39 encoding GPCR-A family and eight encoding GPCR-B family. In addition, we assessed the tissue expression profile of 33 GPCRs (27 GPCR-As and six GPCR-Bs) transcripts. These GPCRs were found to be widely expressed in different tissues. Similar to the expression profiles of neuropeptides, 20 of these putative GPCR-encoding transcripts were also detected in the ovary. This is the first study to establish the identify of neuropeptides and their GPCRs in P. trituberculatus, and provide information for further investigations into the effect of neuropeptides on the physiology and behavior of decapod crustaceans.

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“…The first strand of cDNA was synthesized with 1 μg of total RNA using the SMARTer RACE cDNA Amplification Kit (Clontech, Kusatsu, Japan). Gene-specific primers ( Table S1 ) were designed by Primer Premier 5.0 software based on the homologous sequence in the brain transcriptome database ( 32 ). RACE-PCR amplification was conducted with initial denaturation at 94°C for 30 s, followed by 35 cycles at 94°C for 5 s, 60°C for 30 s, and 72°C for 2 min, then a final elongation at 72°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Crustacean neuropeptides are important neuroendocrine factors generally synthesized and secreted from the central nerve system (CNS) such as the brain and the X-organ/sinus gland complex in eyestalks, then transported to the target tissue through hemolymph to govern a variety of critical physiological processes including metabolism, molting, growth, and reproduction ( 4 – 7 ). As unique neuroendocrine factors, many crustacean neuropeptides have attracted wide attention in the regulation of ovarian development ( 1 , 4 , 8 ). It has been well known that the neuropeptides in eyestalks exhibit inhibitory effect on the gonadal development.…”

Section: Introductionmentioning

confidence: 99%

Evidences for Red Pigment Concentrating Hormone (RPCH) and Beta-Pigment Dispersing Hormone (β-PDH) Inducing Oocyte Meiotic Maturation in the Chinese Mitten Crab, Eriocheir sinensis

et al. 2021

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Red pigment concentrating hormone (RPCH) and pigment dispersing hormone (PDH) are crustacean neuropeptides involved in broad physiological processes including body color changes, circadian rhythm, and ovarian growth. In this study, the full-length cDNA of RPCH and PDH were identified from the brain of the Chinese mitten crab Eriocheir sinensis. The deduced RPCH and PDH mature peptides shared identical sequence to the adipokinetic hormone/RPCH peptides family and the β-PDH isoforms and were designated as Es-RPCH and Es-β-PDH, respectively. Es-RPCH and Es-β-PDH transcripts were distributed in the brain and eyestalks. The positive signals of Es-RPCH and Es-β-PDH were localized in the neuronal clusters 6, 8, 9, 10, and 17 of the brain as revealed by in situ hybridization. The expression level of Es-RPCH and Es-β-PDH mRNA in nervous tissues were all significantly increased at vitellogenic stage, and then decreased at the final meiotic maturation stage. The administrated with synthesized Es-RPCH peptide results in germinal vesicles shift toward the plasma membrane in vitellogenic oocyte, and significant decrease of the gonad-somatic index (GSI) and mean oocyte diameter as well as the expression of vitellogenin mRNA at 30 days post injection in vivo. Similar results were also found when injection of the Es-β-PDH peptide. In vitro culture demonstrated that Es-RPCH and Es-β-PDH induced germinal vesicle breakdown of the late vitellogenic oocytes. Comparative ovarian transcriptome analysis indicated that some reproduction/meiosis-related genes such as cdc2 kinase, cyclin B, 5-HT-R and retinoid-X receptor were significantly upregulated in response to Es-RPCH and Es-β-PDH treatments. Taken together, these results provided the evidence for the inductive effect of Es-RPCH and Es-β-PDH on the oocyte meiotic maturation in E. sinensis.

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Transcriptome analysis of the brain of the Chinese mitten crab, Eriocheir sinensis, for neuropeptide abundance profiles during ovarian development

Cited by 15 publications

References 29 publications

Signaling Pathways That Regulate the Crustacean Molting Gland

Signaling Pathways That Regulate the Crustacean Molting Gland

Identification and characterization of expression profiles of neuropeptides and their GPCRs in the swimming crab, Portunus trituberculatus

Evidences for Red Pigment Concentrating Hormone (RPCH) and Beta-Pigment Dispersing Hormone (β-PDH) Inducing Oocyte Meiotic Maturation in the Chinese Mitten Crab, Eriocheir sinensis

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