Transcriptome analysis of the Amazonian viper Bothrops atrox venom gland using expressed sequence tags (ESTs)

Neiva, Márcia; Arraes, Fabrício Barbosa Monteiro; Souza, Jonso Vieira de; Rádis‐Baptista, Gandhi; Silva, Álvaro Rossan de Brandão Prieto da; Walter, Maria Emília M. T.; Brigido, Marcelo M.; Yamane, Toshimi; López-Lozano, J.L.; Astolfi-Filho, Spártaco

doi:10.1016/j.toxicon.2009.01.006

Cited by 54 publications

(46 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The overall protein composition of these pit viper venoms is in agreement with their pharmacological properties, e.g. the elevated quantity of P-III SVMPs expressed by the venom gland of these snakes, may correlate with the severity of the hemorrhagic reactions evoked by their toxins, as evidenced in the transcriptome analysis of the Amazonian B. atrox venom [49].…”

Section: Discussionmentioning

confidence: 53%

Exploring the proteomes of the venoms of the Peruvian pit vipers Bothrops atrox, B. barnetti and B. pictus

Kohlhoff

Borges

Yarlequé

et al. 2012

Journal of Proteomics

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 53%

Exploring the proteomes of the venoms of the Peruvian pit vipers Bothrops atrox, B. barnetti and B. pictus

Kohlhoff

Borges

Yarlequé

et al. 2012

Journal of Proteomics

View full text Add to dashboard Cite

“…In opposition, in venoms from snakes collected in Brazil, Ecuador and Peru, PIII-SVMPs were the most abundant toxin, corresponding to the paedomorphic phenotype [15,16,18]. In Brazilian Amazon, PIII-SVMPs were certainly the major toxins with relative concentrations in venoms ranging around 50% both in proteomic or transcriptomics studies [15,19,20].…”

Section: Introductionmentioning

confidence: 98%

Comparison of venoms from wild and long-term captive Bothrops atrox snakes and characterization of Batroxrhagin, the predominant class PIII metalloproteinase from the venom of this species

et al. 2015

View full text Add to dashboard Cite

Comparisons between venoms from snakes kept under captivity or collected at the natural environment are of fundamental importance in order to obtain effective antivenoms to treat human victims of snakebites. In this study, we compared composition and biological activities of Bothrops atrox venom from snakes collected at Tapajós National Forest (Pará State, Brazil) or maintained for more than 10 years under captivity at Instituto Butantan herpetarium after have been collected mostly at Maranhão State, Brazil. Venoms from captive or wild snakes were similar except for small quantitative differences detected in peaks correspondent to phospholipases A2 (PLA2), snake venom metalloproteinases (SVMP) class PI and serine proteinases (SVSP), which did not correlate with fibrinolytic and coagulant activities (induced by PI-SVMPs and SVSPs). In both pools, the major toxic component corresponded to PIII-SVMPs, which were isolated and characterized. The characterization by mass spectrometry of both samples identified peptides that matched with a single PIII-SVMP cDNA characterized by transcriptomics, named Batroxrhagin. Sequence alignments show a strong similarity between Batroxrhagin and Jararhagin (96%). Batroxrhagin samples isolated from venoms of wild or captive snakes were not pro-coagulant, but inhibited collagen-induced platelet-aggregation, and induced hemorrhage and fibrin lysis with similar doses. Results suggest that in spite of environmental differences, venom variability was detected only among the less abundant components. In opposition, the most abundant toxin, which is a PIII-SVMP related to the key effects of the venom, is structurally conserved in the venoms. This observation is relevant for explaining the efficacy of antivenoms produced with venoms from captive snakes in human accidents inflicted at distinct natural environments.

show abstract

“…Besides large-scale EST libraries available for several organs of the anole lizard (including a brain library, dbEST library #23338, yet to be analyzed), reptilian transcriptomes so far are quite limited: a few snake venom-gland partial transcriptomes (each consisting of 600 to 1,000 ESTs generally clustering into about 300 unique sequences [20-22]), a heart transcriptome of the Burmese python consisting of about 2,800 mRNAs [23], 3,064 assembled unique sequences of Alligator missipiensis analyzed for their GC-content [24], and 833 assembled unique sequences available for the red-eared slider turtle, with a few related to brain development [25,26]. A notable very recent exception is a garter snake large-scale multi-individual and multi-organ transcriptome [27], which identified about 13,000 snake genes on the basis of homology assignment with other vertebrates, as well as thousands of transcripts of unidentified protein-coding genes.…”

Section: Introductionmentioning

confidence: 99%

Reptilian-transcriptome v1.0, a glimpse in the brain transcriptome of five divergent Sauropsida lineages and the phylogenetic position of turtles

Tzika

Helaers

Schramm

et al. 2011

EvoDevo

View full text Add to dashboard Cite

BackgroundReptiles are largely under-represented in comparative genomics despite the fact that they are substantially more diverse in many respects than mammals. Given the high divergence of reptiles from classical model species, next-generation sequencing of their transcriptomes is an approach of choice for gene identification and annotation.ResultsHere, we use 454 technology to sequence the brain transcriptome of four divergent reptilian and one reference avian species: the Nile crocodile, the corn snake, the bearded dragon, the red-eared turtle, and the chicken. Using an in-house pipeline for recursive similarity searches of >3,000,000 reads against multiple databases from 7 reference vertebrates, we compile a reptilian comparative transcriptomics dataset, with homology assignment for 20,000 to 31,000 transcripts per species and a cumulated non-redundant sequence length of 248.6 Mbases. Our approach identifies the majority (87%) of chicken brain transcripts and about 50% of de novo assembled reptilian transcripts. In addition to 57,502 microsatellite loci, we identify thousands of SNP and indel polymorphisms for population genetic and linkage analyses. We also build very large multiple alignments for Sauropsida and mammals (two million residues per species) and perform extensive phylogenetic analyses suggesting that turtles are not basal living reptiles but are rather associated with Archosaurians, hence, potentially answering a long-standing question in the phylogeny of Amniotes.ConclusionsThe reptilian transcriptome (freely available at http://www.reptilian-transcriptomes.org) should prove a useful new resource as reptiles are becoming important new models for comparative genomics, ecology, and evolutionary developmental genetics.

show abstract

Transcriptome analysis of the Amazonian viper Bothrops atrox venom gland using expressed sequence tags (ESTs)

Cited by 54 publications

References 63 publications

Exploring the proteomes of the venoms of the Peruvian pit vipers Bothrops atrox, B. barnetti and B. pictus

Exploring the proteomes of the venoms of the Peruvian pit vipers Bothrops atrox, B. barnetti and B. pictus

Comparison of venoms from wild and long-term captive Bothrops atrox snakes and characterization of Batroxrhagin, the predominant class PIII metalloproteinase from the venom of this species

Reptilian-transcriptome v1.0, a glimpse in the brain transcriptome of five divergent Sauropsida lineages and the phylogenetic position of turtles

Contact Info

Product

Resources

About