Transcriptome analysis of Spodoptera litura reveals the molecular mechanism to pyrethroids resistance

Xu, Li; Yu, Mei; Liu, Runqiang; Chen, Xiling; Li, Dong-Zhi; Wang, Chengju

doi:10.1016/j.pestbp.2020.104649

Cited by 40 publications

(42 citation statements)

References 40 publications

(34 reference statements)

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“…Similar results were also obtained in the species S. litura. Indeed, strains resistant to fenvalerate, beta-cypermethrin and cyhalothrin had their resistance reduced by the use of PBO [45]. RNAseq experiments for these field-collected Chinese strains (LF and NJ) showed over-expression of several P450s including CYP9As.…”

Section: Resistance Through Over-expression Of Cyp9amentioning

confidence: 99%

Resistance in the Genus Spodoptera: Key Insect Detoxification Genes

Hilliou

Chertemps

Maı̈bèche

et al. 2021

Insects

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The genus Spodoptera (Lepidoptera: Noctuidae) includes species that are among the most important crop pests in the world. These polyphagous species are able to feed on many plants, including corn, rice and cotton. In addition to their ability to adapt to toxic compounds produced by plants, they have developed resistance to the chemical insecticides used for their control. One of the main mechanisms developed by insects to become resistant involves detoxification enzymes. In this review, we illustrate some examples of the role of major families of detoxification enzymes such as cytochromes P450, carboxyl/cholinesterases, glutathione S-transferases (GST) and transporters such as ATP-binding cassette (ABC) transporters in insecticide resistance. We compare available data for four species, Spodoptera exigua, S. frugiperda, S. littoralis and S. litura. Molecular mechanisms underlying the involvement of these genes in resistance will be described, including the duplication of the CYP9A cluster, over-expression of GST epsilon or point mutations in acetylcholinesterase and ABCC2. This review is not intended to be exhaustive but to highlight the key roles of certain genes.

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Section: Resistance Through Over-expression Of Cyp9amentioning

confidence: 99%

Resistance in the Genus Spodoptera: Key Insect Detoxification Genes

Hilliou

Chertemps

Maı̈bèche

et al. 2021

Insects

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“…The role of ABC transporters in efflux pumps, facilitating cellular excretion of insecticides or metabolites, strengthens a hypothesis for their playing a role in insecticide resistance in insects ( Rösner & Merzendorfer, 2020 ). In a recent study of S. litura resistance to pyrethroid, the ABC5 gene was significantly upregulated and showed a strong correlation with insecticide resistance ( Xu et al, 2020 ). In B. dorslis , ABC transporter genes might play roles in the insecticide resistance, with several bdABC genes significantly upregulated after treatment of B. dorsalis with malathion, abamectin, and beta-cypermethrin ( Xiao et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Gene selection for studying frugivore-plant interactions: a review and an example using Queensland fruit fly in tomato

Roohigohar

Clarke

Prentis

2021

PeerJ

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Fruit production is negatively affected by a wide range of frugivorous insects, among them tephritid fruit flies are one of the most important. As a replacement for pesticide-based controls, enhancing natural fruit resistance through biotechnology approaches is a poorly researched but promising alternative. The use of quantitative reverse transcription PCR (RT-qPCR) is an approach to studying gene expression which has been widely used in studying plant resistance to pathogens and non-frugivorous insect herbivores, and offers a starting point for fruit fly studies. In this paper, we develop a gene selection pipe-line for known induced-defense genes in tomato fruit, Solanum lycopersicum, and putative detoxification genes in Queensland fruit fly, Bactrocera tryoni, as a basis for future RT-qPCR research. The pipeline started with a literature review on plant/herbivore and plant/pathogen molecular interactions. With respect to the fly, this was then followed by the identification of gene families known to be associated with insect resistance to toxins, and then individual genes through reference to annotated B. tryoni transcriptomes and gene identity matching with related species. In contrast for tomato, a much better studied species, individual defense genes could be identified directly through literature research. For B. tryoni, gene selection was then further refined through gene expression studies. Ultimately 28 putative detoxification genes from cytochrome P450 (P450), carboxylesterase (CarE), glutathione S-transferases (GST), and ATP binding cassette transporters (ABC) gene families were identified for B. tryoni, and 15 induced defense genes from receptor-like kinase (RLK), D-mannose/L-galactose, mitogen-activated protein kinase (MAPK), lipoxygenase (LOX), gamma-aminobutyric acid (GABA) pathways and polyphenol oxidase (PPO), proteinase inhibitors (PI) and resistance (R) gene families were identified from tomato fruit. The developed gene selection process for B. tryoni can be applied to other herbivorous and frugivorous insect pests so long as the minimum necessary genomic information, an annotated transcriptome, is available.

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“…A relative susceptible population (GX), provided by Guangxi Tianyuan Biochemistry Corp., Ltd and maintained in the laboratory for more than 10 years, was used for the resistance level comparison. The rear condition was same with that in Xu et al 24…”

Section: Insect Culturementioning

confidence: 99%

“…95.0% chlorpyrifos and 91.0% phoxim were obtained from Beijing Huarong Biochemical Co., Ltd (Beijing, China). Topical application as described in Xu et al 24 was used. Briefly, chlorpyrifos and phoxim were dissolved in acetone to five or six gradient concentrations and 1 μL was applied on the thoracic dorsum of third-instar larvae.…”

Section: Bioassaymentioning

confidence: 99%

“…23 Silencing of GSTe1 in S. litura by RNAi increases the cumulative mortality to fenvalerate significantly. 24 In addition, the activity of GSTs in the epsilon class can be inhibited by some insecticides. The recombinant enzyme activity of SlGSTE7 and SlGSTE20 can be inhibited by permethrin, bendiocarb, diazinon and chlorfenapyr, especially for diazinon.…”

Section: Introductionmentioning

confidence: 99%

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SlGSTE9 participates in the stability of chlorpyrifos resistance in Spodoptera litura

Xie

et al. 2021

Pest Management Science

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BACKGROUND: Spodoptera litura is an important agricultural pest and has developed serious resistance to multiple insecticides. The resistance level to several insecticides is reported to be unstable, but the mechanism is less reported.RESULTS: Chlorpyrifos and phoxim resistance level in a field-collected population of S. litura declined continuously from the first to the tenth generation and remained stable at the 11th and 12th generations without insecticide exposure. Synergist experiment showed that diethyl maleate and piperonyl butoxide significantly increased mortality to chlorpyrifos and phoxim in the first and sixth generations, but not in the 12th generation. The expression of 31 identified glutathione S-transferase (GST) genes in the third-instar larvae of S. litura in the first, sixth and 12th generations was determined, and eight genes were seen to decrease significantly in the sixth and 12th generations compared with the first generation. SlGSTe9 was selected for further functional study as it had higher abundance and significantly higher expression in the chlorpyrifos-resistant population than in the susceptible population. The recombinant protein of SlGSTE9 showed metabolism activity to chlorpyrifos in vitro and in Escherichia coli, but not to phoxim. Silencing of SlGSTe9 increased the cumulative mortality to chlorpyrifos significantly. SlGSTE9 also showed antioxidant activity to cumene hydroperoxide. CONCLUSION: Our results suggest that SlGSTe9 is directly involved in chlorpyrifos resistance stability, but not in phoxim. SlGSTE9 may also participate in insecticides resistance by relieving the oxidase stress induced by insecticides.

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Transcriptome analysis of Spodoptera litura reveals the molecular mechanism to pyrethroids resistance

Cited by 40 publications

References 40 publications

Resistance in the Genus Spodoptera: Key Insect Detoxification Genes

Resistance in the Genus Spodoptera: Key Insect Detoxification Genes

Gene selection for studying frugivore-plant interactions: a review and an example using Queensland fruit fly in tomato

SlGSTE9 participates in the stability of chlorpyrifos resistance in Spodoptera litura

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