Transcriptome analysis of Spodoptera frugiperda 9 (Sf9) cells infected with baculovirus, AcMNPV or AcMNPV-BmK IT

Wei, Lili; Cao, Leixi; Miao, Yanyan; Shu-ju, WU; Xu, Sihua; Wang, Rui-Sheng; Du, Jun; Liang, Aihua; Fu, Yuejun

doi:10.1007/s10529-017-2356-8

Cited by 20 publications

(16 citation statements)

References 14 publications

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“…2). This is consistent with the trend observed in previous transcriptomic studies in cell cultures of AcMNPV-infected S. frugiperda and T. ni [33,34,36,45,46]. Interestingly, we see extensive similarities across multiple biological processes as deduced from the functional attributes of DETs in each species, suggesting a shared host response to the AcMNPV pathogen.…”

Section: Host Transcriptomic Responses To the Acmnpv Infectionsupporting

confidence: 91%

“…Most studies exploring transcriptional regulation of these host-pathogen interactions use cell cultures infected with the virus. The transcriptome responses of S. frugiperda [33,34] and T. ni [4,35] cell cultures infected with AcMNPV have shown quite divergent transcriptional profiles, which makes it difficult to deduce the impact of these responses in intact organisms. Recently, Shrestha et al, (2019) described the in vivo transcriptional response of T. ni during AcMNPV infection.…”

Section: Introductionmentioning

confidence: 99%

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A tale of two transcriptomic responses in agricultural pests via host defenses and viral replication

Pantha

Subbaiah

et al. 2020

Preprint

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BackgroundAutographa californica Multiple Nucleopolyhedrovirus (AcMNPV) is a baculovirus with a high potential for its use as a biopesticide against arthropod pests. The budded form of the virus causes a systemic infection when it escapes the midgut to enter the hemolymph of susceptible hosts. Yet, the specific molecular processes underlying the biocidal activity of AcMNPV on its insect hosts are largely unknown.ResultsIn this study, we describe the transcriptional responses in two major pests, Spodoptera frugiperda and Trichoplusia ni, to determine the host-pathogen responses during AcMNPV infection, concurrently with the viral response to the host. We assembled species-specific de novo reference transcriptomes of the hemolymph to identify key transcripts that respond during pathogenesis in these arthropod models where genomic resources are sparse. We found that the suppression of transcriptional processes related to chitin, a metabolite critical for basement membrane stability and tracheal development are central in establishing a systemic infection. Synergistic transcriptional support was observed to suggest suppression of immune responses and induction of oxidative stress indicating disease progression in the host. The entire AcMNPV core genome was expressed in the host hemolymph and viral genes predominantly associated with the budded virus replication, structure, and movement were more abundant than those associated with the occlusion-derived virus. Genes known to directly arrest host cell cycle and development were among the most abundant AcMNPV transcripts in infected hosts. Interestingly, several of the host genes (e.g. Chitin synthase) that were targeted by the pathogen as revealed by our study are also targets of several chemical insecticides currently used commercially to control arthropod pests.ConclusionsOur results reveal an extensive overlap between biological processes represented by genes differently expressed in both hosts, as well as convergence on highly abundant viral genes expressed in the two hosts, providing an overview of the host-pathogen transcriptomic landscape during systemic infection. Given the diversity of AcMNPV strains that infect a wide range of insect hosts, our study provides a framework where pathogen strains could be selected to target specific host genes that facilitates modulation of the infection strength and specificity of the susceptible hosts.

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Section: Host Transcriptomic Responses To the Acmnpv Infectionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

A tale of two transcriptomic responses in agricultural pests via host defenses and viral replication

Pantha

Subbaiah

et al. 2020

Preprint

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“…RNA-Seq of each samples was performed by Sangon Biotech Co. Ltd. (Shanghai). Methods of RNA-Seq analysis were described in previous studies [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Potential Cardiovascular Protective Targets of the Thyroid Hormone Metabolite 3-Iodothyronamine (3-T1AM)

Zhou

Zhang

et al. 2020

BioMed Research International

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Background. The thyroid hormone metabolite 3-iodothyronamine (3-T1AM) is rapidly emerging as a promising compound in decreasing the heart rate and lowering the cardiac output. The aim of our study was to fully understand the molecular mechanism of 3-T1AM on cardiomyocytes and its potential targets in cardiovascular diseases. Materials and Methods. In our study, we utilized RNA-Seq to characterize the gene expression in H9C2 cells after 3-T1AM treatment. Comparative transcriptome analysis, including gene ontology, signaling pathways, disease connectivity analysis, and protein-protein interaction networks (PPI), was presented to find the critical gene function, hub genes, and related pathways. Results. A total of 1494 differently expressed genes (DEGs) were identified (192 upregulated and 1302 downregulated genes) in H9C2 cells for 3-T1AM treatment. Of these, 90 genes were associated with cardiovascular diseases. The PPI analysis indicated that 5 hub genes might be the targets of 3-T1AM. Subsequently, eight DEGs characterized using RNA-Seq were confirmed by RT-qPCR assays. Conclusions. Our study provides a comprehensive analysis of 3-T1AM on H9C2 cells and delineates a new insight into the therapeutic intervention of 3-T1AM for the cardiovascular diseases.

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“…frugiperda samples, which contained different developmental time-points and tissues, using NGS 1 . Furthermore, the transcriptome of Sf9 was obtained, and the insecticidal mechanisms of AcMNPV-BmK IT and AcMNPV treatment have been explained 38 . In the present study, we assembled the transcriptome of the S .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin

Shu

Zhang

Veeran

et al. 2017

Sci Rep

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As an important botanical pesticide, azadirachtin demonstrates broad insecticidal activity against many agricultural pests. The results of a previous study indicated the toxicity and apoptosis induction of azadirachtin in Spodoptera frugiperda Sf9 cells. However, the lack of genomic data has hindered a deeper investigation of apoptosis in Sf9 cells at a molecular level. In the present study, the complete transcriptome data for Sf9 cell line was accomplished using Illumina sequencing technology, and 97 putative apoptosis-related genes were identified through BLAST and KEGG orthologue annotations. Fragments of potential candidate apoptosis-related genes were cloned, and the mRNA expression patterns of ten identified genes regulated by azadirachtin were examined using qRT-PCR. Furthermore, Western blot analysis showed that six putative apoptosis-related proteins were upregulated after being treated with azadirachtin while the protein Bcl-2 were downregulated. These data suggested that both intrinsic and extrinsic apoptotic signal pathways comprising the identified potential apoptosis-related genes were potentially active in S. frugiperda. In addition, the preliminary results revealed that caspase-dependent or caspase-independent apoptotic pathways could function in azadirachtin-induced apoptosis in Sf9 cells.

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Transcriptome analysis of Spodoptera frugiperda 9 (Sf9) cells infected with baculovirus, AcMNPV or AcMNPV-BmK IT

Abstract: These results provide a broad spectrum of candidate genes that are critically involved in the molecular regulation mechanism of Sf9 cells infected with AcMNPV-BmK IT.

Cited by 20 publications

References 14 publications

A tale of two transcriptomic responses in agricultural pests via host defenses and viral replication

A tale of two transcriptomic responses in agricultural pests via host defenses and viral replication

Comparative Transcriptome Analysis Reveals the Potential Cardiovascular Protective Targets of the Thyroid Hormone Metabolite 3-Iodothyronamine (3-T1AM)

Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin

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