Transcriptome analysis of skeletal muscle at prenatal stages in Polled Dorset versus Small-tailed Han sheep

Abstract

ABSTRACT. The objectives of the present study were to identify additional genes that may play important roles in the regulation of skeletal muscle growth and development, and to provide fundamental information for understanding the underlying molecular mechanisms. Eighteen cDNA libraries were constructed from the longissimus muscle of Polled Dorset (PD) and Small-tailed Han (SH) fetuses. To reveal the differences between the two species, we analyzed the differences in gene expression in 60-, 90-and 120-day fetal skeletal muscle by applying Agilent ovine genome-wide microarray. In this study, we obtained 17,704 genes using a chip containing 39,242 probes. There were 88 differentially expressed genes in the 60-day group (P < 0.05), 128 genes in the 90-day group (P < 0.05), and 340 genes in the 120-day group (P < 0.05) between the two breeds. The differentially expressed genes were grouped in different GO categories and signaling pathways. These results suggested that there are many genetic differences in the muscle growth and development transcriptomes between these two breeds. This study laid the foundation for future genomic research in sheep.

Comparative Transcriptome Analysis Identifying the Different Molecular Genetic Markers Related to Production Performance and Meat Quality in Longissimus Dorsi Tissues of MG × STH and STH Sheep

“…Comparative transcriptome analyses have also identified some important differentially expressed genes (DEGs), particularly metallothionein 3 (MMP3), related to a curly fleece in Chinese Tan sheep [13]. Overall, the genome-wide screening of crossbred and indigenous sheep breeds has identified some specific trait-associated genetic molecules, including MYOD1, MYOG, and IGFBPs [1,3,14]. However, understanding of the differences between the phenotypic and genetic aspects of different sheep breeds remains limited.…”

Integrative Meta-Analysis: Unveiling Genetic Factors in Meat Sheep Growth and Muscular Development through QTL and Transcriptome Studies

“…Additionally, they needed to provide measures of sample variance or the necessary information to calculate it (such as test statistics and p -values). As a result, the final dataset comprised 7 studies extracted from comprehensive research papers [ 12 , 21 , 22 , 23 , 24 , 25 ]. The detailed workflow is illustrated in Figure 1 and Supplementary File S1, Table S1 .…”

“…Among the 11 identified studies, seven only included one biological replicate per breed. Therefore, there were seven datasets selected for biological analysis [ 12 , 21 , 22 , 23 , 24 , 25 ]. These datasets consisted of samples obtained from the muscle tissue of adult male sheep, with at least four biological replicates per breed.…”

Tissue Engineering for Clean Meat Production

“…Microarrays quantify 10 1 -10 6 known RNA transcripts, and can be used to obtain an overview of gene expression patterns in muscle and fat (Wang et al, 2005;Tan et al, 2006). Genome-wide microarrays, which were used to profile gene expression in fat and muscle, are commercially available from Affymetrix and Agilent for bovine (Lee et al, 2010;Cassar-Malek et al, 2017;Hayashi et al, 2018;Liu et al, 2018), ovine (Liu et al, 2015;González-Calvo et al, 2017), porcine (Fontanesi et al, 2011;González-Prendes et al, 2017a,b) and chicken (Du et al, 2017;Zahoor et al, 2017;Chang et al, 2018) samples, however may be too expensive and provide an overwhelming volume of data for routine sample assessment. Additional commercial products for porcine samples were summarized by Pena et al (2014), including microarrays specific for muscle.…”