Transcriptome analysis of roots from resistant and susceptible rice varieties infected with Hirschmanniella mucronata

Abstract

Hirschmanniella mucronata is a plant‐parasitic nematode that is widespread in rice production areas and causes 10–25% yield losses a year on average. Here, we investigated the mechanism of resistance to this nematode by comparing the transcriptomes of roots from resistant (Jiabali) and susceptible (Bawangbian) varieties of rice. Of 39 233 unigenes, 2243. exhibited altered total expression levels between control and infected resistant and susceptible varieties. Significant differences were observed in the expression levels of genes related to stress, peptidase regulation or inhibition, oxidoreductase activity, peroxidase activity and antioxidant activity. The up‐regulated genes related to plant secondary metabolites, such as phenylpropanoid, lignin, cellulose or hemicellulose, may result in an increase in the degree of resistance of Jiabali to the H. mucronata infection compared with that of Bawangbian by affecting cell wall organization or biogenesis. Of the genes that responded similarly to H. mucronata infection, ~252 (~76.59%) showed greater changes (whether induced or suppressed) in RN155 (susceptible varieties infected by rice root nematode) than in RN51 (resistance varieties infected by rice root nematode). Nineteen pathogenesis‐related genes belonging to nine pathogenesis‐related gene families were significantly induced by H. mucronata in the infected roots of Jiabali and Bawangbian, and 13 differentially expressed genes showed changes in their abundance only in the susceptible Bawangbian variety. This study may help enhance our understanding of the mechanisms underlying plant resistance to nematodes.

Comparative transcriptome profiling of resistant and susceptible foxtail millet responses to Sclerospora graminicola infection

“…Han et al analysed the early transcriptome of rice in response to infection with Ustilaginoidea virens and found that 16 peroxidase genes were up-regulated in the resistant variety IR28 and down-regulated in the susceptible variety LYP9 [ 45 ]. Similar research was reported for the interaction between rice and Hirschmanniella mucronata [ 46 ], peanut and Puccinia arachidis [ 47 ] , and mulberry and Meloidogyne enterolobii based on transcriptome analyses [ 48 ]. Cytochrome P450 is a supergene family containing haem monooxygenases involved in many metabolic pathways and defence reactions in plants [ 49 ].…”

Diverse Roles of MAX1 Homologues in Rice

“…Two other genes, Os02g0754400 (MR = 95.812) and Os10g0191300 (MR = 81.425) play a role in response to arsenic [83] and silicon [157], respectively (Table 3). However, it was postulated that, Os10g0191300, which encodes a pathogenesis-related protein (Table S10), might be involved in rice defence against insects [158] and fungi [157]. Other pathogen resistance genes are Os03g0760500 (MR = 96.156)-against bacteria [120], and Os10g0335000 (MR = 81.994)-against fungi [159].…”

Comparative transcriptome profiling of resistant and susceptible foxtail millet responses to Sclerospora graminicola infection

“…Han et al analysed the early transcriptome of rice in response to infection with Ustilaginoidea virens and found that 16 peroxidase genes were upregulated in the resistant variety IR28 and downregulated in the susceptible variety LYP9 [43]. Similar research was reported in the transcriptome of the interaction between rice and Hirschmanniella mucronata [44], peanut and Puccinia arachidis [45], and Mulberry and Meloidogyne enterolobii [46]. Cytochrome P450 is a supergene family containing haem monooxygenases in many metabolic pathways and defence reactions in plants [47].…”