Transcriptome analysis of rice leaves in response to Rhizoctonia solani infection and reveals a novel regulatory mechanism

Yuan, De Peng; Xu, Xiao; Hong, Woo Jong; Wang, Si Ting; Jia, Xin; Liu, Yang; Li, Shuang; Li, Zhi Min; Qi-zhong, Sun; Mei, Qiong; Li, Shuai; Jung, Ki‐Hong; Wei, Song; Xuan, Yuan

doi:10.1007/s11816-020-00630-9

Cited by 38 publications

(27 citation statements)

References 69 publications

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“…Various TFs belonging to NAC, MYB and ERF TF families were more up-regulated after R. solani infection (Supplemental Table 2). In line with these observations, another recent study also suggested the involvement of MYB and NAC TFs in rice SB responses (Peng Yuan et al, 2020). To summarize, WRKY, NAC, ERF and MYB TFs are highly induced in rice upon R. solani infection.…”

Section: Discussionsupporting

confidence: 70%

Differential Regulation of Rice Transcriptome toRhizoctonia solaniinfection

Das

Mazahar

Sahu

et al. 2021

Preprint

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Sheath Blight (SB) disease in rice crop caused by the infection of the fungal pathogen Rhizoctonia solani (R. solani) is one of the severe rice diseases that can cause up to 50% yield losses. Naturally occurring rice varieties resistant to SB have not been reported yet. We have performed a Time-Series RNA-Seq analysis on a widely cultivated rice variety BPT-5204 for identifying its transcriptomic response signatures to R. solani infection at 1st, 2nd and 5th day post inoculation (dpi). In total, 428, 3225 and 1225 genes were differentially expressed in the treated rice plants post 1, 2 and 5 dpi, respectively. GO and KEGG enrichment analysis identified significant processes and pathways differentially altered in the rice plant after the fungal infection. Machine learning and network based integrative approach was used to construct Transcriptional Regulatory Networks (TRNs) of the rice plant at the three Time Points. Regulatory network analysis identified SUB1B, MYB30 and CCA1 as important regulatory hub Transcription Factors in rice during R. solani infection. Jasmonic acid signaling pathway was activated and in contrast, photosynthesis and carbon fixation processes were significantly compromised. Involvement of MAPK, CYPs, Peroxidases and PAL genes was observed in response to the fungal infection. Circadian clock was also strongly influenced by R. solani infection. Our integrative analysis identified 7 putative SB resistant genes altered in rice after R. solani infection and provided a better understanding of rice plant response to R. solani infection.

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Section: Discussionsupporting

confidence: 70%

Differential Regulation of Rice Transcriptome toRhizoctonia solaniinfection

Das

Mazahar

Sahu

et al. 2021

Preprint

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“…However, the role of SWEET genes in rice and Rhizoctonia solani , the causative agent of sheath blight disease (ShB), is largely unknown. Our previous transcriptome data showed that SWEET14 expression is sensitive to R. solani infection (De Peng Yuan, 2020). qPCR results verified that R. solani infection dramatically induces SWEET14 expression along with a pathogen‐related protein PBZ1 (Figure 1a).…”

Section: Figurementioning

confidence: 99%

Tissue‐specific activation of DOF11 promotes rice resistance to sheath blight disease and increases grain weight via activation of SWEET14

Kim

Xue

Song

et al. 2020

Plant Biotechnology Journal

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“…The up regulation of this plethora of factors involved in biotic stress response signalling indicates an induction of certain defence pathways at both time points analysed. However, a recent analysis of significantly changed expression level of rice genes in response to R. solani AG1 IA also revealed negative effects of the transcription factor genes OsWRKY53 and OsAKT1 on the defence response [ 25 ]. This supports the necessity that involvement of DEGs in a specific defence reply has yet to be determined, either with mutant plants altered in specific signalling components or with the analysis of potato varieties, showing a different degree of quantitative response when challenged by R. solani .…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Changes in Potato Sprouts upon Interaction with Rhizoctonia solani Indicate Pathogen-Induced Interference in the Defence Pathways of Potato

Zrenner

Verwaaijen

Genzel

et al. 2021

IJMS

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Rhizoctonia solani is the causer of black scurf disease on potatoes and is responsible for high economical losses in global agriculture. In order to increase the limited knowledge of the plants’ molecular response to this pathogen, we inoculated potatoes with R. solani AG3-PT isolate Ben3 and carried out RNA sequencing with total RNA extracted from potato sprouts at three and eight days post inoculation (dpi). In this dual RNA-sequencing experiment, the necrotrophic lifestyle of R. solani AG3-PT during early phases of interaction with its host has already been characterised. Here the potato plants’ comprehensive transcriptional response to inoculation with R. solani AG3 was evaluated for the first time based on significantly different expressed plant genes extracted with DESeq analysis. Overall, 1640 genes were differentially expressed, comparing control (−Rs) and with R. solani AG3-PT isolate Ben3 inoculated plants (+Rs). Genes involved in the production of anti-fungal proteins and secondary metabolites with antifungal properties were significantly up regulated upon inoculation with R. solani. Gene ontology (GO) terms involved in the regulation of hormone levels (i.e., ethylene (ET) and jasmonic acid (JA) at 3 dpi and salicylic acid (SA) and JA response pathways at 8 dpi) were significantly enriched. Contrastingly, the GO term “response to abiotic stimulus” was down regulated at both time points analysed. These results may support future breeding efforts toward the development of cultivars with higher resistance level to black scurf disease or the development of new control strategies.

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Transcriptome analysis of rice leaves in response to Rhizoctonia solani infection and reveals a novel regulatory mechanism

Cited by 38 publications

References 69 publications

Differential Regulation of Rice Transcriptome toRhizoctonia solaniinfection

Differential Regulation of Rice Transcriptome toRhizoctonia solaniinfection

Tissue‐specific activation of DOF11 promotes rice resistance to sheath blight disease and increases grain weight via activation of SWEET14

Transcriptional Changes in Potato Sprouts upon Interaction with Rhizoctonia solani Indicate Pathogen-Induced Interference in the Defence Pathways of Potato

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