Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle)

Zhang, Jinyong; He, Muchun; Liu, Changlin; Liu, Shufang; Zhuang, Zhimeng

doi:10.1016/j.aqrep.2023.101494

Cited by 1 publication

(2 citation statements)

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“…Previous studies on S. pharaonis mainly focused on biology and optimization of culture conditions [ 5 – 7 ], to date, little information has been available on the nature of gene transcripts involved in Sepia gonad development and reproductive cycle [ 8 ]. Our recent studies have demonstrated that ovarian development in S. pharaonis is not synchronous and can be categorized into five stages: the non-developmental stage, the developmental stage, the near-maturity stage, the maturity stage, and the degeneration stage [ 9 ]. However, in the course of conducting a comprehensive study on the full-scale captive breeding of S. pharaonis , we identified several challenges that limit the scale of captive breeding for this species.…”

Section: Introductionmentioning

confidence: 99%

“…Transcriptomic analyses of cuttlefish ovaries provide contemporary insights into breeding fertility and contribute significantly to the field of developmental biology [ 10 – 12 ]. Molecular investigations into the ovaries of female Sepiella primarily focus on the transcriptomic analysis of both immature and mature developmental stages [ 8 , 9 ]. Many genes, such as those encoding vitellogenin, vitellogenin receptor, GnRH receptor, estrogen receptor, and FMRFamide receptor, have been identified and demonstrated to have important roles in ovary maturation [ 10 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptomic analysis of the different developmental stages of ovary in the cuttlefish Sepia pharaonis

Jiang,

Han,

et al. 2024

BMC Genomics

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The cuttlefish, Sepia pharaonis, is characterized by rapid growth and strong disease resistance, making it an important commercially farmed cephalopod species in the southeastern coastal regions of China. However, in the reproductive process of S. pharaonis, there are challenges such as a low output of eggs, poor quality, and low survival rates of newly hatched juveniles. Therefore, there is an urgent need to study the molecular mechanisms underlying ovarian development in this species. In this study, we conducted the first transcriptomic analysis of the ovary at four developmental stages: the undeveloped stage, developing stage, nearly-ripe stage, and ripe stage, and compared the transcriptomics among these four stages using Illumina sequencing technology. The total numbers of clean reads of the four stages ranged from 40,890,772 to 52,055,714 reads. A total of 136,829 DEGs were obtained, GC base ratios of raw data were between 38.44 and 44.59%, and the number of uniquely mapped reads spanned from 88.08 to 95.90%. The Pearson correlation coefficient demonstrated a strong correlation among different samples within the same group, PCA and Anosim analysis also revealed that the grouping of these four stages was feasible, and each stage could be distinguished from the others. GO enrichment analysis demonstrated that ovarian follicle growth, sex differentiation, and transforming growth factor beta receptor, played a foreshadowing role at the early ovarian development stage, and the terms of small molecule metabolic process, peptide metabolic process, and catalytic activity were prominent at the mature stage. Meanwhile, KEGG analysis showed that the early ovarian development of S. pharaonis was mainly associated with the cell cycle, DNA replication, and carbon metabolism, while the mid-late ovarian development was involved with the signal transduction, endocrine system, and reproduction pathway. RT-qPCR further confirmed the consistent expression patterns of genes such as 17β-HSD, GH, VGS, NFR, and NYR in the ovaries of S. pharaonis, exhibiting elevated levels of expression during the maturation stage. Conversely, ER and OM exhibited high expression levels during the early stages of ovarian development. These transcriptomic data provide insights into the molecular mechanisms of S. pharaonis ovarian development. The findings of this study will contribute to improving the reproduction and development of cuttlefish and enriching the bioinformatics knowledge of cephalopods.

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