Transcriptome analysis of primary chicken cells infected with infectious bronchitis virus strain K047-12 isolated in Korea

Lee, Rangyeon; Jung, Jin‐Woo; Yeo, Ji-in; Kwon, Hyun-Jung; Park, Jeongho

doi:10.1007/s00705-021-05124-9

Cited by 5 publications

(8 citation statements)

References 51 publications

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“…Pathways such as Cytokine-cytokine receptor interaction and Toll-like receptor signaling have top ranked functions correlated to gamma CoV infection in avian cells (Fig. 2 ) 34 .…”

Section: Discussionmentioning

confidence: 99%

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Differing coronavirus genres alter shared host signaling pathways upon viral infection

Cruz-Pulido

Ouma

Kenney

2022

Sci Rep

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Coronaviruses are important viral pathogens across a range of animal species including humans. They have a high potential for cross-species transmission as evidenced by the emergence of COVID-19 and may be the origin of future pandemics. There is therefore an urgent need to study coronaviruses in depth and to identify new therapeutic targets. This study shows that distant coronaviruses such as Alpha-, Beta-, and Deltacoronaviruses can share common host immune associated pathways and genes. Differentially expressed genes (DEGs) in the transcription profile of epithelial cell lines infected with swine acute diarrhea syndrome, severe acute respiratory syndrome coronavirus 2, or porcine deltacoronavirus, showed that DEGs within 10 common immune associated pathways were upregulated upon infection. Twenty Three pathways and 21 DEGs across 10 immune response associated pathways were shared by these viruses. These 21 DEGs can serve as focused targets for therapeutics against newly emerging coronaviruses. We were able to show that even though there is a positive correlation between PDCoV and SARS-CoV-2 infections, these viruses could be using different strategies for efficient replication in their cells from their natural hosts. To the best of our knowledge, this is the first report of comparative host transcriptome analysis across distant coronavirus genres.

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“…Pathways such as Cytokine-cytokine receptor interaction and Toll-like receptor signaling have top ranked functions correlated to gamma CoV infection in avian cells (Fig. 2 ) 34 .…”

Section: Discussionmentioning

confidence: 99%

“…Liu et al provided a transcriptomic profiling of long non-coding RNAs (lncRNAs) in swine testicular (ST) cells infected with PDCoV 33 . Finally, for gammacoronaviruses, Lee et al investigated changes in chicken embryonic kidney (CEK) cells infected with infectious bronchitis virus (IBV) by transcriptome analysis 34 .…”

Section: Introductionmentioning

confidence: 99%

Differing coronavirus genres alter shared host signaling pathways upon viral infection

Cruz-Pulido

Ouma

Kenney

2022

Sci Rep

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“…Since the early 2000s, researchers have aimed to map the host gene expression patterns involved in IBV infection [ 37 , 38 , 39 ]. More recent transcriptomic studies have looked at chicken spleen tissues [ 40 , 41 , 42 ], tracheal tissues [ 43 , 44 , 45 ], lung tissues [ 41 ], human lung epithelial-like cells [ 46 ], chicken kidney tissues and cells [ 47 , 48 , 49 , 50 , 51 ], dendritic cells [ 52 , 53 ], macrophages [ 54 ], and fibroblasts [ 55 ] upon infection with various strains of IBV. Currently, there are no RNA-seq studies specifically looking at IBV infection in chicken tracheal epithelial cells (cTECs), nor using the IBV DMV/1639 strain, which has been the dominant IBV genotype circulating in Canada [ 21 , 22 ] and the United States of America (USA) in recent years [ 20 , 56 ].…”

Section: Introductionmentioning

confidence: 99%

Host Immune Response Modulation in Avian Coronavirus Infection: Tracheal Transcriptome Profiling In Vitro and In Vivo

O’Dowd,

Isham,

Vatandour

et al. 2024

Viruses

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Infectious bronchitis virus (IBV) is a highly contagious Gammacoronavirus causing moderate to severe respiratory infection in chickens. Understanding the initial antiviral response in the respiratory mucosa is crucial for controlling viral spread. We aimed to characterize the impact of IBV Delmarva (DMV)/1639 and IBV Massachusetts (Mass) 41 at the primary site of infection, namely, in chicken tracheal epithelial cells (cTECs) in vitro and the trachea in vivo. We hypothesized that some elements of the induced antiviral responses are distinct in both infection models. We inoculated cTECs and infected young specific pathogen-free (SPF) chickens with IBV DMV/1639 or IBV Mass41, along with mock-inoculated controls, and studied the transcriptome using RNA-sequencing (RNA-seq) at 3 and 18 h post-infection (hpi) for cTECs and at 4 and 11 days post-infection (dpi) in the trachea. We showed that IBV DMV/1639 and IBV Mass41 replicate in cTECs in vitro and the trachea in vivo, inducing host mRNA expression profiles that are strain- and time-dependent. We demonstrated the different gene expression patterns between in vitro and in vivo tracheal IBV infection. Ultimately, characterizing host–pathogen interactions with various IBV strains reveals potential mechanisms for inducing and modulating the immune response during IBV infection in the chicken trachea.

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“…Transcriptomic analysis is a powerful tool for elucidating the molecular interactions between viruses and hosts. Transcriptomic analysis aids the identification of biological processes or crucial host proteins that mediate virus-host interactions at the molecular level ( Wang et al, 2016 ; Shimizu et al, 2018 ; Cao et al, 2019 ; Lee et al, 2021 ; Zhang et al, 2022 ). Transcriptomic technologies, especially RNA-seq, have widespread application in elucidating bacteriophage-host interactions ( Jian et al, 2016 ; Mojardín and Salas, 2016 ; Morimoto et al, 2018 ; Yang et al, 2019 ; Zhong et al, 2020 ; Brandão et al, 2021 ; Zhang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and transcriptomic analysis of a novel polymycovirus in the fungus Talaromyces amestolkiae

Teng

Chen²,

Hu³

et al. 2022

Front. Microbiol.

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Talaromyces amestolkiae is an important fungal species owing to its ubiquity in soils, plants, air, and food. In this study, we identified a novel six-segmented polymycovirus, Talaromyces amestolkiae polymycovirus 1 (TaPmV-1). Each of the double-stranded (ds) RNA segments of TaPmV-1 contained a single open reading frame, and the proteins encoded by dsRNA1, dsRNA2, dsRNA3, and dsRNA 5 shared significant amino acid identities of 56, 40, 47, and 43%, respectively, with the corresponding proteins of Aspergillus fumigatus polymycovirus-1(AfuPmV-1). DsRNA1, dsRNA3, and dsRNA5 of TaPmV-1 encoded an RNA-dependent RNA polymerase (RdRp), a viral methyltransferase, and a PAS-rich protein, respectively. The functions of the proteins encoded by dsRNA2, dsRNA4, and dsRNA6 have not been elucidated. Comparison of the virus-infected strain LSH3 with virus-cured strain LSHVF revealed that infection with TaPmV-l may reduce the production of red pigments and induce the clustering of fungal sclerotia. Furthermore, transcriptomic analyses demonstrated that infection with TaPmV-l downregulated the expression of transcripts related to metabolism, and may correlate with the reduced production of red pigments and clustering of sclerotia in T. amestolkiae. These results of this study provide novel insights into the mechanism of fungal gene regulation by polymycovirus infections at the transcriptome level, and this study is the first to report a novel polymycovirus of T. amestolkiae.

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Transcriptome analysis of primary chicken cells infected with infectious bronchitis virus strain K047-12 isolated in Korea

Cited by 5 publications

References 51 publications

Differing coronavirus genres alter shared host signaling pathways upon viral infection

Differing coronavirus genres alter shared host signaling pathways upon viral infection

Host Immune Response Modulation in Avian Coronavirus Infection: Tracheal Transcriptome Profiling In Vitro and In Vivo

Molecular characterization and transcriptomic analysis of a novel polymycovirus in the fungus Talaromyces amestolkiae

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