Transcriptome analysis of MYB transcription factors family and PgMYB genes involved in salt stress resistance in Panax ginseng

Abstract

Background As the king of all herbs, the medicinal value of ginseng is self-evident. The perennial nature of ginseng causes its quality to be influenced by various factors, one of which is the soil environment. During plant growth and development, MYB transcription factors play an important role in responding to abiotic stresses and regulating the synthesis of secondary metabolites. However, there are relatively few reports on the MYB transcription factor family in Panax ginseng. Results This study identified 420 PgMYB transcripts under 117 genes ID in the Jilin ginseng transcriptome database. Phylogenetic analysis showed that PgMYB transcripts in Jilin ginseng were classified into 19 functional subclasses. The GO annotation result indicated that the functional differentiation of PgMYB transcripts was annotated to 11 functional nodes at GO Level 2 in ginseng. Expression pattern analysis of PgMYB transcripts based on the expression data (TPM) that PgMYB transcripts were revealed spatiotemporally specific in expression patterns. We performed a weighted network co-expression network analysis on the expression of PgMYB transcripts from different samples. The co-expression network containing 51 PgMYB transcripts was formed under a soft threshold of 0.85, revealing the reciprocal relationship of PgMYB in ginseng. Treatment of adventitious roots of ginseng with different concentrations of NaCl revealed four up-regulated expression of PgMYB transcripts that can candidate genes for salt resistance studies in ginseng. Conclusions The present findings provide data resources for the subsequent study of the functions of MYB transcription factor family members in ginseng, and provide an experimental basis for the anti-salt functions of MYB transcription factors in Panax ginseng.

Transcriptome-Wide Identification and Integrated Analysis of a UGT Gene Involved in Ginsenoside Ro Biosynthesis in Panax ginseng

“…In order to improve the ginsenoside content of P. ginseng , several genes from different gene families capable of affecting the synthesis of ginsenosides were identified and analyzed, such as, bHLHs [ 36 ], bZIPs [ 37 ], P450s [ 38 ], GRASs [ 39 ], and MYBs [ 40 ]. With the development of synthetic biology, the heterologous synthesis of ginsenosides in microbial cell factories offers a sustainable solution [ 41 , 42 , 43 , 44 ].…”

Transcriptome-Wide Integrated Analysis of the PgGT25-04 Gene in Controlling Ginsenoside Biosynthesis in Panax ginseng

“…More than 30 key enzyme genes of the ginsenoside biosynthesis pathway have been identified from plants and microorganisms of the genus Ginseng [ 35 ], examples of which include farnesyl pyrophosphate synthase ( FPS ), squalene epoxidase ( SQE ), oxidative squalene cyclase ( OSC ), cytochrome P450s ( CYP450 ), UDP-glycosyltransferases ( UGT ), etc. Many transcription factors have been identified in ginseng, such as NF-Y [ 36 ], MYB [ 37 ], bHLH [ 38 ], AP2/ERF [ 39 ], GRAS [ 40 ], NAC [ 41 ] and WRKY [ 42 ], and most of them respond to abiotic stresses such as salt stress and methyl jasmonate signaling, while some are also involved in ginseng secondary metabolic processes [ 43 ]. In contrast, studies on the involvement of Trihelix genes in ginseng secondary metabolism have not been reported.…”

Genome-wide characterization, evolutionary analysis, and expression pattern analysis of the trihelix transcription factor family and gene expression analysis under MeJA treatment in Panax ginseng

“…Under normal conditions, transcription factors with binding function and can be to other proteins domain or DNA sequence promoter region to perform their specific functions [ 2 ]. Currently, the GRAS [ 3 , 4 ], AP2/ERF [ 5 ], MYB [ 6 ], WRKY [ 7 ], NF-Y [ 8 ], HD‑Zip [ 9 ], bHLH [ 10 ], and other specific transcription factor families have been bioinformatically analysed, and their functions have been verified in ginseng. The trihelix transcription factor had been functionally validated in many plants, but no in-depth reports or systematic studies about this gene family in ginseng.…”