Transcriptome analysis of metabolisms related to fruit cracking during ripening of a cracking-susceptible grape berry cv. Xiangfei (Vitis vinifera L.)

Zhu, Mingtao; Yu, Jun; Zhao, Min; Wang, Meijun; Gao, Yang

doi:10.1007/s13258-020-00930-y

Cited by 16 publications

(13 citation statements)

References 58 publications

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“…Another approach is sorting berries into density classes by floating them in salt solutions (Bigard et al 2019). However, recent studies about developmental changes in enzyme activities and gene expression profiles continued using calendar-or phenology-based sampling methods (Famiani et al 2018, Fasoli et al 2018, Savoi et al 2019, Zhu et al 2020. Therefore, we describe here a reliable method to study the sequence of key changes that occur at the onset of and during grape berry ripening.…”

Section: Introductionmentioning

confidence: 99%

Soft, Sweet, and Colorful: Stratified Sampling Reveals Sequence of Events at the Onset of Grape Ripening

et al. 2020

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Asynchronous development of grape berries leads to high variation among berry samples collected during veraison. We applied a stratified sampling method that groups berries by firmness to the touch and visible skin color to study the sequence of physical and chemical changes occurring at the onset of and during ripening. Method robustness and reproducibility were tested by measuring berry weight, diameter, deformation, and total soluble solids (TSS) in samples collected from ten wine, table, and juice grape cultivars varying in skin color. Additionally, Syrah berries sampled by five different individuals were compared in one year, and Syrah and Merlot berry samples and cluster images were evaluated over three years to account for environmental

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Section: Introductionmentioning

confidence: 99%

Soft, Sweet, and Colorful: Stratified Sampling Reveals Sequence of Events at the Onset of Grape Ripening

et al. 2020

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“…Previous studies have shown that the composition of the cell wall in the peel influences the structure of the peel (Huang et al, 1999 ; Yang et al, 2016 ). Calcium may have reduced cell wall-related enzyme activity and expression of related genes by decreasing ABA content in grapes, thereby reducing water-soluble sugar content (Yu et al, 2019 ; Zhu et al, 2020 ). In this study, calcium is closely related to cell wall components, cell wall-related enzyme activity, and related genes ( Figures 2 , 3 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of CaCl2 Sprays in Different Fruit Development Stages on Grape Berry Cracking

Shi

Zhou²,

Qin³

et al. 2022

Front. Plant Sci.

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Grape cracking is a common phenomenon that can reach more than 50% in some varieties and can cause enormous economic losses. “Li Xiu” grapes in different developmental stages were treated with calcium chloride (5 g/L), and the cracking rate and related biochemical and genetic indices were measured in the fruit ripening stage. The results showed that calcium treatment during the flowering period could significantly reduce grape cracking. Based on the experimental results, there are several reasons as follows: first, calcium spraying during the flowering period was more favorable to calcium absorption in grapes, and the increased calcium content in the peels helped to improve the mechanical properties of the peels, thus increasing crack resistance; second, calcium treatment reduced the expression levels of genes related to glucose metabolism, which in turn reduced PG and cellulase activities, delaying the degradation of pectin and cellulose and resulting in more structural integrity of the peels; third, calcium treatment increased fruit hardness and reduced fruit ventral pressure by decreasing the expression levels of ABA-related genes and synthesis of endogenous abscisic acid (ABA), soluble sugars (SSs), and total soluble solids (TSSs).

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“…Transcriptomes can produce large amounts of data, which is helpful for comprehensively and rapidly revealing the molecular mechanism of fruit cracking ( Chen et al, 2019 ; Jiang H. K. et al, 2019 ; Wang et al, 2019a ; Niu et al, 2020 ; Zhu et al, 2020 ); however, research using this technology has not previously been reported for seed cracking in T. grandis . Here, transcriptome sequencing was performed on 6 T. grandis aril samples at two different growth stages using RNA-Seq.…”

Section: Discussionmentioning

confidence: 99%

Acceleration of Aril Cracking by Ethylene in Torreya grandis During Nut Maturation

Gao

Shen

et al. 2021

Front. Plant Sci.

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Torreya grandis ‘Merrillii’ is a famous nut with great nutritional value and high medicinal value. Aril cracking is an important process for seed dispersal, which is also an indicator of seed maturation. However, the cracking mechanism of T. grandis aril during the maturation stage remains largely unknown. Here, we provided a comprehensive view of the physiological and molecular levels of aril cracking in T. grandis by systematically analyzing its anatomical structure, physiological parameters, and transcriptomic response during the cracking process. These results showed that the length of both epidermal and parenchymatous cell layers significantly increased from 133 to 144 days after seed protrusion (DASP), followed by a clear separation between parenchymatous cell layers and kernel, which was accompanied by a breakage between epidermal and parenchymatous cell layers. Moreover, analyses of cell wall composition showed that a significant degradation of cellular wall polysaccharides occurred during aril cracking. To examine the global gene expression changes in arils during the cracking process, the transcriptomes (96 and 141 DASP) were analyzed. KEGG pathway analysis of DEGs revealed that 4 of the top 10 enriched pathways were involved in cell wall modification and 2 pathways were related to ethylene biosynthesis and ethylene signal transduction. Furthermore, combining the analysis results of co-expression networks between different transcription factors, cell wall modification genes, and exogenous ethylene treatments suggested that the ethylene signal transcription factors (ERF11 and ERF1A) were involved in aril cracking of T. grandis by regulation of EXP and PME. Our findings provided new insights into the aril cracking trait in T. grandis.

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Transcriptome analysis of metabolisms related to fruit cracking during ripening of a cracking-susceptible grape berry cv. Xiangfei (Vitis vinifera L.)

Cited by 16 publications

References 58 publications

Soft, Sweet, and Colorful: Stratified Sampling Reveals Sequence of Events at the Onset of Grape Ripening

Soft, Sweet, and Colorful: Stratified Sampling Reveals Sequence of Events at the Onset of Grape Ripening

Effect of CaCl2 Sprays in Different Fruit Development Stages on Grape Berry Cracking

Acceleration of Aril Cracking by Ethylene in Torreya grandis During Nut Maturation

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