Transcriptome analysis of maize pathogen Fusarium verticillioides revealed FvLcp1, a secreted protein with type-D fungal LysM and chitin-binding domains, that plays important roles in pathogenesis and mycotoxin production

Zhang, Huan; Kim, Man S.; Huang, Jun; Huang, Yan; Yang, Tao; Song, Linlin; Shim, Won Bo

doi:10.1016/j.micres.2022.127195

Cited by 3 publications

(2 citation statements)

References 74 publications

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“…The ability to suppress BAX-triggered cell death is a well-known technique for the initial screening of fungal candidate effectors [ 18 , 34 , 35 ]. To evaluate the function of the above candidate effectors, we further selected the above 18 candidate effector genes for transient expression in N. benthamiana using an Agrobacterium -mediated transformation system.…”

Section: Resultsmentioning

confidence: 99%

Screening of Candidate Effectors from Magnaporthe oryzae by In Vitro Secretomic Analysis

Shi

et al. 2023

IJMS

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Magnaporthe oryzae is the causal agent of rice blast, one of the most serious diseases of rice worldwide. Secreted proteins play essential roles during a M. oryzae–rice interaction. Although much progress has been made in recent decades, it is still necessary to systematically explore M. oryzae-secreted proteins and to analyze their functions. This study employs a shotgun-based proteomic analysis to investigate the in vitro secretome of M. oryzae by spraying fungus conidia onto the PVDF membrane to mimic the early stages of infection, during which 3315 non-redundant secreted proteins were identified. Among these proteins, 9.6% (319) and 24.7% (818) are classified as classically or non-classically secreted proteins, while the remaining 1988 proteins (60.0%) are secreted through currently unknown secretory pathway. Functional characteristics analysis show that 257 (7.8%) and 90 (2.7%) secreted proteins are annotated as CAZymes and candidate effectors, respectively. Eighteen candidate effectors are selected for further experimental validation. All 18 genes encoding candidate effectors are significantly up- or down-regulated during the early infection process. Sixteen of the eighteen candidate effectors cause the suppression of BAX-mediated cell death in Nicotiana benthamiana by using an Agrobacterium-mediated transient expression assay, suggesting their involvement in pathogenicity related to secretion effectors. Our results provide high-quality experimental secretome data of M. oryzae and will expand our knowledge on the molecular mechanisms of M. oryzae pathogenesis.

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Section: Resultsmentioning

confidence: 99%

Screening of Candidate Effectors from Magnaporthe oryzae by In Vitro Secretomic Analysis

Shi

et al. 2023

IJMS

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“…In addition to the potential health risks to humans, fumonisin consumed by animals causes diseases, such as leukoencephalomalacia in horses and pulmonary edema in pigs. Significant efforts have been made in control food deterioration or contamination caused by fungi, with the traditional pesticide’s application being the most commonly used strategy in production process. − However, the use of traditional pesticides has adversely affected wildlife and humans, in addition to environmental and agricultural pollution, ecological imbalances, and food safety impacts, including residues, resistance, and moisture return. Traditional pesticides have become unsuitable for the sustainable development in modern society .…”

Section: Introductionmentioning

confidence: 99%

Discovery and Development of Luvangetin from Zanthoxylum avicennae as a New Fungicide Candidate for Fusarium verticillioides

Chen,

Zhou,

Liu

et al. 2024

J. Agric. Food Chem.

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Pathogenic fungi pose a significant threat to crop yields and human healthy, and the subsequent fungicide resistance has greatly aggravated these agricultural and medical challenges. Hence, the development of new fungicides with higher efficiency and greater environmental friendliness is urgently required. In this study, luvangetin, isolated and identified from the root of Zanthoxylum avicennae, exhibited wide-spectrum antifungal activity in vivo and in vitro. Integrated omics and in vitro and in vivo transcriptional analyses revealed that luvangetin inhibited GAL4-like Zn(II) 2 Cys6 transcriptional factor-mediated transcription, particularly the FvFUM21-mediated FUM cluster gene expression, and decreased the biosynthesis of fumonisins inFusarium verticillioides. Moreover, luvangetin binds to the double-stranded DNA helix in vitro in the groove mode. We isolated and identified luvangetin, a natural metabolite from a traditional Chinese edible medicinal plant and uncovered its multipathogen resistance mechanism. This study is the first to reveal the mechanism underlying the antifungal activity of luvangetin and provides a promising direction for the future use of plant-derived natural products to prevent and control plant and animal pathogenic fungi.

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Comprehensive analysis of the LysM protein family and functional characterization of the key LysM effector StLysM1, which modulates plant immunity in Setosphaeria turcica1

Gong,

Han,

Zhang

et al. 2024

Journal of Integrative Agriculture

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Transcriptome analysis of maize pathogen Fusarium verticillioides revealed FvLcp1, a secreted protein with type-D fungal LysM and chitin-binding domains, that plays important roles in pathogenesis and mycotoxin production

Cited by 3 publications

References 74 publications

Screening of Candidate Effectors from Magnaporthe oryzae by In Vitro Secretomic Analysis

Screening of Candidate Effectors from Magnaporthe oryzae by In Vitro Secretomic Analysis

Discovery and Development of Luvangetin from Zanthoxylum avicennae as a New Fungicide Candidate for Fusarium verticillioides

Comprehensive analysis of the LysM protein family and functional characterization of the key LysM effector StLysM1, which modulates plant immunity in Setosphaeria turcica1

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