Transcriptome analysis of low-dose ionizing radiation-impacted genes in CD4<sup>+</sup> T-cells undergoing activation and regulation of their expression of select cytokines

Cho, Seong‐Jun; Kang, Hana; Hong, Eun-Hee; Kim, Ji Young; Nam, Sang Yong

doi:10.1080/1547691x.2018.1521484

Cited by 15 publications

(17 citation statements)

References 34 publications

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“… 25 It was also determined that LDIR affects cytokine gene expression by different T cells. 26 Only 1 study explored the effects of LDIR on white and red blood cell populations in the context of atherosclerosis, 19 but specific monocyte and lymphocyte subgroups were not investigated. This represents a significant knowledge gap, since monocytes and T cells can polarize into distinct classes that are considered to be either pro-inflammatory or anti-inflammatory, with potentially opposing effects on atherosclerosis.…”

Section: Introductionmentioning

confidence: 99%

Exposure to Low to Moderate Doses of Ionizing Radiation Induces A Reduction of Pro-Inflammatory Ly6chigh Monocytes and a U-Curved Response of T Cells in APOE -/- Mice

et al. 2021

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Low dose ionizing radiation (LDIR) is known to have a protective effect on atherosclerosis in rodent studies, but how it impacts different cells types involved in lesion formation remains incompletely understood. We investigated the immunomodulatory response of different doses and dose-rates of irradiation in ApoE-/- mice. Mice were exposed to external γ rays at very low (1.4 mGy.h-1) or low (50 mGy.h-1) dose-rates, with cumulative doses spanning 50 to 1000 mGy. Flow cytometry of circulating cells revealed a significant decrease in pro-inflammatory Ly6CHi monocytes at all cumulative doses at low dose-rate, but more disparate effects at very low dose-rate with reductions in Ly6CHi cells at doses of 50, 100 and 750 mGy only. In contrast, Ly6CLo monocytes were not affected by LDIR. Similarly, proportions of CD4+ T cell subsets in the spleen did not differ between irradiated mice and non-irradiated controls, whether assessing CD25+FoxP3+ regulatory or CD69+ activated lymphocytes. In the aorta, gene expression of cytokines such as IL-1 and TGF-ß and adhesion molecules such as E-Selectin, ICAM-1, and VCAM-1 were reduced at the intermediate dose of 200 mGy. These results suggest that LDIR may reduce atherosclerotic plaque formation by selectively reducing blood pro-inflammatory monocytes and by impairing adhesion molecule expression and inflammatory processes in the vessel wall. In contrast, splenic T lymphocytes were not affected by LDIR. Furthermore, some responses to irradiation were nonlinear; reductions in aortic gene expression were significant at intermediate doses, but not at either highest or lowest doses. This work furthers our understanding of the impact of LDIR with different dose-rates on immune system response in the context of atherosclerosis.

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Section: Introductionmentioning

confidence: 99%

Exposure to Low to Moderate Doses of Ionizing Radiation Induces A Reduction of Pro-Inflammatory Ly6chigh Monocytes and a U-Curved Response of T Cells in APOE -/- Mice

et al. 2021

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“…As the expression of GPAT correlates with the immunogenic activity of B cells, LDR might help B cell in acquiring immunity. A transcriptome analysis confirmed LDR-induced (0.05 Gy) expression of IFN-γ, IL-4, and IL-6 ( Cho et al, 2018 ). In the same study, LDR also induced the expression of genes involved in mRNA translation, mitochondrial function, cell cycle regulation, and cytokine induction.…”

Section: The Organ-specific Effects Of Ldr In Cell and Mouse Modelsmentioning

confidence: 76%

Organ-Specific Effects of Low Dose Radiation Exposure: A Comprehensive Review

et al. 2020

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Ionizing radiation (IR) is a high-energy radiation whose biological effects depend on the irradiation doses. Low-dose radiation (LDR) is delivered during medical diagnoses or by an exposure to radioactive elements and has been linked to the occurrence of chronic diseases, such as leukemia and cardiovascular diseases. Though epidemiological research is indispensable for predicting and dealing with LDR-induced abnormalities in individuals exposed to LDR, little is known about epidemiological markers of LDR exposure. Moreover, difference in the LDR-induced molecular events in each organ has been an obstacle to a thorough investigation of the LDR effects and a validation of the experimental results in in vivo models. In this review, we summarized the recent reports on LDR-induced risk of organ-specifically arranged the alterations for a comprehensive understanding of the biological effects of LDR. We suggested that LDR basically caused the accumulation of DNA damages, controlled systemic immune systems, induced oxidative damages on peripheral organs, and even benefited the viability in some organs. Furthermore, we concluded that understanding of organ-specific responses and the biological markers involved in the responses is needed to investigate the precise biological effects of LDR.

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“…This effect has also been observed at higher acute doses, with acute 0.2 Gy IR increasing antioxidants in the blood and tissues of rats [127]. Increases in anti-inflammatory cytokines such as IL-4, IL-10, and reductions in inflammatory cy-tokines such as TFN-α have been observed at 50 mGy [158]; however, this effect has also been observed at higher doses of up to 1 Gy [25,159,160]. Of note, one study found that 100 mGy actually increased inflammatory cytokines [161], and another found that 100 mGy may have a negative effect on cognition, although noted further investigation is required [108].…”

Section: Figurementioning

confidence: 85%

Control of Neuroinflammation through Radiation-Induced Microglial Changes

Boyd

Byrne

Middleton

et al. 2021

Cells

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Microglia, the innate immune cells of the central nervous system, play a pivotal role in the modulation of neuroinflammation. Neuroinflammation has been implicated in many diseases of the CNS, including Alzheimer’s disease and Parkinson’s disease. It is well documented that microglial activation, initiated by a variety of stressors, can trigger a potentially destructive neuroinflammatory response via the release of pro-inflammatory molecules, and reactive oxygen and nitrogen species. However, the potential anti-inflammatory and neuroprotective effects that microglia are also thought to exhibit have been under-investigated. The application of ionising radiation at different doses and dose schedules may reveal novel methods for the control of microglial response to stressors, potentially highlighting avenues for treatment of neuroinflammation associated CNS disorders, such as Alzheimer’s disease and Parkinson’s disease. There remains a need to characterise the response of microglia to radiation, particularly low dose ionising radiation.

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Transcriptome analysis of low-dose ionizing radiation-impacted genes in CD4⁺ T-cells undergoing activation and regulation of their expression of select cytokines

Cited by 15 publications

References 34 publications

Exposure to Low to Moderate Doses of Ionizing Radiation Induces A Reduction of Pro-Inflammatory Ly6chigh Monocytes and a U-Curved Response of T Cells in APOE -/- Mice

Exposure to Low to Moderate Doses of Ionizing Radiation Induces A Reduction of Pro-Inflammatory Ly6chigh Monocytes and a U-Curved Response of T Cells in APOE -/- Mice

Organ-Specific Effects of Low Dose Radiation Exposure: A Comprehensive Review

Control of Neuroinflammation through Radiation-Induced Microglial Changes

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Transcriptome analysis of low-dose ionizing radiation-impacted genes in CD4+ T-cells undergoing activation and regulation of their expression of select cytokines

Cited by 15 publications

References 34 publications

Exposure to Low to Moderate Doses of Ionizing Radiation Induces A Reduction of Pro-Inflammatory Ly6chigh Monocytes and a U-Curved Response of T Cells in APOE -/- Mice

Exposure to Low to Moderate Doses of Ionizing Radiation Induces A Reduction of Pro-Inflammatory Ly6chigh Monocytes and a U-Curved Response of T Cells in APOE -/- Mice

Organ-Specific Effects of Low Dose Radiation Exposure: A Comprehensive Review

Control of Neuroinflammation through Radiation-Induced Microglial Changes

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Product

Resources

About

Transcriptome analysis of low-dose ionizing radiation-impacted genes in CD4⁺ T-cells undergoing activation and regulation of their expression of select cytokines