Transcriptome Analysis of Infection of the Archaeon
            <i>Sulfolobus solfataricus</i>
            with
            <i>Sulfolobus</i>
            Turreted Icosahedral Virus

Ortmann, Alice C.; Brumfield, Susan K.; Walther, Jasper; McInnerney, Kathleen; Brouns, Stan J. J.; Werken, Harmen J.G. van de; Bothner, Brian; Douglas, Trevor; Oost, John van der; Young, Mark

doi:10.1128/jvi.02583-07

Cited by 82 publications

(74 citation statements)

References 30 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5), whereas cells arrested by acetate showed elevated levels of Orc1-2 and low levels of Orc1-1 and Orc1-3 (1); resumption of growth after arrest resulted in elevated Orc1-1 and -3 and greatly reduced Orc1-2. Several laboratories have reported microarray analyses of the response of the Sulfolobus transcriptome to a range of physiological stresses such as UV treatment, viral infection, and heat shock (21)(22)(23)(24). Interestingly, these studies have revealed modulation of Orc1 gene transcript and/or protein levels; similar to that seen with microarray analysis of acetate-treated cells (25).…”

Section: Discussionmentioning

confidence: 48%

Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius

Duggin

McCallum

Bell

2008

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The ''baby machine'' provides a means of generating synchronized cultures of minimally perturbed cells. We describe the use of this technique to establish the key cell-cycle parameters of hyperthermophilic archaea of the genus Sulfolobus. The 3 DNA replication origins of Sulfolobus acidocaldarius were mapped by 2D gel analysis to near 0 (oriC2), 579 (oriC1), and 1,197 kb (oriC3) on the 2,226-kb circular genome, and we present a direct demonstration of their activity within the first few minutes of a synchronous cell cycle. We also detected X-shaped DNA molecules at the origins in log-phase cells, but these were not directly associated with replication initiation or ongoing chromosome replication in synchronized cells. Whole-genome marker frequency analyses of both synchronous and log-phase cultures showed that origin utilization was close to 100% for all 3 origins per round of replication. However, oriC2 was activated slightly later on average compared with oriC1 and oriC3. The DNA replication forks moved bidirectionally away from each origin at Ϸ88 bp per second in synchronous culture. Analysis of the 3 Orc1/Cdc6 initiator proteins showed a uniformity of cellular abundance and origin binding throughout the cell cycle. In contrast, although levels of the MCM helicase were constant across the cell cycle, its origin localization was regulated, because it was strongly enriched at all 3 origins in early S phase.Archaea ͉ cell cycle ͉ DNA replication

show abstract

Section: Discussionmentioning

confidence: 48%

Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius

Duggin

McCallum

Bell

2008

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Except for a few isolated cases (4)(5)(6), it is generally presumed that these viruses persist in the host cell in a carrier state, a nonlytic relationship in which virions are continuously secreted by the still-dividing cells (7). However, the classification of crenarchaeal viruses as chronic is based on indirect experimental evidence, such as a lack of optical density (OD) decrease and absence of cellular debris in infected cultures (e.g., 8,9).…”

mentioning

confidence: 99%

A unique virus release mechanism in the Archaea

Bize

Karlsson

Ekefjärd

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

124

172

View full text Add to dashboard Cite

Little is known about the infection cycles of viruses infecting cells from Archaea, the third domain of life. Here, we demonstrate that the virions of the archaeal Sulfolobus islandicus rod-shaped virus 2 (SIRV2) are released from the host cell through a mechanism, involving the formation of specific cellular structures. Large pyramidal virus-induced protrusions transect the cell envelope at several positions, rupturing the S-layer; they eventually open out, thus creating large apertures through which virions escape the cell. We also demonstrate that massive degradation of the host chromosomes occurs because of virus infection, and that virion assembly occurs in the cytoplasm. Furthermore, intracellular viral DNA is visualized by flow cytometry. The results show that SIRV2 is a lytic virus, and that the host cell dies as a consequence of elaborated mechanisms orchestrated by the virus. The generation of specific cellular structures for a distinct step of virus life cycle is known in eukaryal virus-host systems but is unprecedented in cells from other domains.lysis ͉ virus factory ͉ hyperthermophile ͉ infection cycle

show abstract

“…STIV1 propagates in Sulfolobus solfataricus strains with a slow life cycle of about 38 h [38]. Viral transcripts are first detected 8 h post infection (p.i.)…”

Section: Turreted Icosahedral Stiv1mentioning

confidence: 99%

“…After 16 h, most viral genes are expressed and, on average, are 12-fold up-regulated ( Figure 5B). After 24 h, several genes, including F93, F75, D66, C63 and B66a, and a few intergenic regions, show little or no transcription [38].…”

Section: Turreted Icosahedral Stiv1mentioning

confidence: 99%

“…All transcript levels peaked at 24 h p.i. The cross-hatched arrows represent intergenic regions (adapted from Ortmann et al [38] [33] and the high sequence conservation and timing of transcription strongly implicate these gene products in viral replication. The virion proteins VP1, VP2 and VP3 are encoded by late transcripts T1/2 and another late transcript, C792, encodes one of two different proteins produced by other fuselloviruses which may generate tail end filaments putatively involved in host receptor recognition ( Figure 5A) [30].…”

Section: Fuselloviruses Ssv1/2mentioning

confidence: 99%

See 1 more Smart Citation

Archaeal viruses—novel, diverse and enigmatic

Peng

Garrett

She

2012

Sci. China Life Sci.

View full text Add to dashboard Cite

Recent research has revealed a remarkable diversity of viruses in archaeal-rich environments where spindles, spheres, filaments and rods are common, together with other exceptional morphotypes never recorded previously. Moreover, their double-stranded DNA genomes carry very few genes exhibiting homology to those of bacterial and eukaryal viruses. Studies on viral life cycles are still at a preliminary stage but important insights are being gained especially from microarray analyses of viral transcripts for a few model virus-host systems. Recently, evidence has been presented for some exceptional archaealnspecific mechanisms for extra-cellular morphological development of virions and for their cellular extrusion. Here we summarise some of the recent developments in this rapidly developing and exciting research area. virus morphotypes, diversity and evolution, life cycle, temporal regulation, cellular extrusion mechanism

show abstract

Transcriptome Analysis of Infection of the Archaeon Sulfolobus solfataricus with Sulfolobus Turreted Icosahedral Virus

Abstract: Microarray analysis of infection by

Cited by 82 publications

References 30 publications

Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius

Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius

A unique virus release mechanism in the Archaea

Archaeal viruses—novel, diverse and enigmatic

Contact Info

Product

Resources

About