Transcriptome Analysis of Infection of the Archaeon
            <i>Sulfolobus solfataricus</i>
            with
            <i>Sulfolobus</i>
            Turreted Icosahedral Virus

Ortmann, Alice C.; Brumfield, Susan K.; Walther, Jasper; McInnerney, Kathleen; Brouns, Stan J. J.; Werken, Harmen J.G. van de; Bothner, Brian; Douglas, Trevor; Oost, John van der; Young, Mark

doi:10.1128/jvi.00912-08

Cited by 24 publications

(53 citation statements)

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“…Perhaps one of the best studied archaeal virus-host systems is the STIV-Sulfolobus model system (Brumfield et al, 2009;Dellas et al, in preparation;Fu and Johnson, 2012;Fu et al, 2010;Khayat et al, 2010Khayat et al, , 2005Larson et al, 2007aLarson et al, , 2007bLarson et al, , 2006Maaty et al, 2006Maaty et al, , 2012Ortmann et al, 2008;Rice et al, 2004;Snyder et al, 2011aSnyder et al, , 2011bSnyder et al, , 2013aSnyder et al, , 2013bVeesler et al, 2013;Wirth et al, 2011). Even though a complete archaeal virus replication cycle has not been solved, several steps of the STIV replication cycle have been described (Brumfield et al, 2009;Dellas et al, in preparation;Fu et al, 2010;Happonen et al, 2013;Snyder et al, 2011bSnyder et al, , 2013b.…”

Section: Archaeal Virus-host Model Systemsmentioning

confidence: 94%

“…The archaeal viruses that have been developed into model systems are Sulfolobus spindle-shaped virus 1 [SSV1; (Stedman et al, 1999)], Sulfolobus islandicus rod-shaped virus 2 [SIRV2; (Prangishvili et al, 1999)], and Sulfolobus turreted icosahedral virus [STIV; (Wirth et al, 2011)]. The availability of these archaeal virushost model systems has resulted in a better understanding of gene function (Clore and Stedman, 2007;Snyder et al, 2011b;Stedman et al, 1999;Wirth et al, 2011), gene expression (Frols et al, 2008;Okutan et al, 2013;Ortmann et al, 2008), protein structures [STIV (Dellas et al, in preparation;Happonen et al, 2013;Khayat et al, 2005;Larson et al, 2007aLarson et al, , 2007bLarson et al, , 2006Rice et al, 2004;Veesler et al, 2013); SSV1 (Kraft et al, 2004a(Kraft et al, , 2004bStedman et al, 2015;Hong et al, 2015); SIRV (Szymczyna et al, 2009)], the development of viral shuttle vectors (Stedman et al, 1999;Wirth et al, 2011), and differential protein expression (Maaty et al, 2012). However, since all of these genetic systems are based exclusively on Sulfolobus host species, we have only a very limited understanding of the range of host-virus interactions in archaeal viruses.…”

Section: Archaeal Virus-host Model Systemsmentioning

confidence: 97%

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40 Years of archaeal virology: Expanding viral diversity

2015

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The first archaeal virus was isolated over 40 years ago prior to the recognition of the three domain structure of life. In the ensuing years, our knowledge of Archaea and their viruses has increased, but they still remain the most mysterious of life's three domains. Currently, over 100 archaeal viruses have been discovered, but few have been described in biochemical or structural detail. However, those that have been characterized have revealed a new world of structural, biochemical and genetic diversity. Several model systems for studying archaeal virus-host interactions have been developed, revealing evolutionary linkages between viruses infecting the three domains of life, new viral lysis systems, and unusual features of host-virus interactions. It is likely that the study of archaeal viruses will continue to provide fertile ground for fundamental discoveries in virus diversity, structure and function.

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Section: Archaeal Virus-host Model Systemsmentioning

confidence: 94%

Section: Archaeal Virus-host Model Systemsmentioning

confidence: 97%

40 Years of archaeal virology: Expanding viral diversity

2015

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“…An infectious clone of STIV was constructed through insertion of the STIV genome into a TOPO vector as previously described (9). The susceptible host Sulfolobus solfataricus 2-2-12 (P2 3 ) was evolved from S. solfataricus P2 as previously described (2).…”

Section: Methodsmentioning

confidence: 99%

Structure-Based Mutagenesis of Sulfolobus Turreted Icosahedral Virus B204 Reveals Essential Residues in the Virion-Associated DNA-Packaging ATPase

Dellas

Snyder

Dills

et al. 2016

J Virol

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Sulfolobus turreted icosahedral virus (STIV), an archaeal virus that infects the hyperthermoacidophile Sulfolobus solfataricus, is one of the most well-studied viruses of the domain Archaea. STIV shares structural, morphological, and sequence similarities with viruses from other domains of life, all of which are thought to belong to the same viral lineage. Several of these common features include a conserved coat protein fold, an internal lipid membrane, and a DNA-packaging ATPase. B204 is the ATPase encoded by STIV and is thought to drive packaging of viral DNA during the replication process. Here, we report the crystal structure of B204 along with the biochemical analysis of B204 mutants chosen based on structural information and sequence conservation patterns observed among members of the same viral lineage and the larger FtsK/HerA superfamily to which B204 belongs. Both in vitro ATPase activity assays and transfection assays with mutant forms of B204 confirmed the essentiality of conserved and nonconserved positions. We also have identified two distinct particle morphologies during an STIV infection that differ in the presence or absence of the B204 protein. The biochemical and structural data presented here are not only informative for the STIV replication process but also can be useful in deciphering DNA-packaging mechanisms for other viruses belonging to this lineage. IMPORTANCE STIV is a virus that infects a host from the domainArchaea that replicates in high-temperature, acidic environments. While STIV has many unique features, there exist several striking similarities between this virus and others that replicate in different environments and infect a broad range of hosts from Bacteria and Eukarya. Aside from structural features shared by viruses from this lineage, there exists a significant level of sequence similarity between the ATPase genes carried by these different viruses; this gene encodes an enzyme thought to provide energy that drives DNA packaging into the virion during infection. The experiments described here highlight the elements of this enzyme that are essential for proper function and also provide supporting evidence that B204 is present in the mature STIV virion. In Sulfolobus turreted icosahedral virus (STIV), the open reading frame B204 (previously described as B164) (1, 2) encodes a putative ATPase that is predicted to function as the molecular motor driving viral DNA packaging during infection (3). Interestingly, it has been demonstrated that viruses harboring ATPases homologous to B204 share other common features, such as a spherical morphology, an internal lipid membrane, and a structurally homologous coat protein fold (termed the double ␤-barrel or double jelly roll fold) (4). The structural and functional assessment of viruses belonging to this putative viral lineage can provide insights on viral infection strategies across the three domains of life. In the case of B204, understanding its function has implications for mechanisms by which an ancient molecular motor may h...

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“…Microarray experiments following an infected culture and comparing it with an uninfected culture have provided insights into the timing of events during viral infection as well as the host's response to the virus [2]. In a near-synchronous single replication cycle, there is little evidence for distinct temporal regulation of viral gene expression.…”

Section: Transcriptional Analysismentioning

confidence: 99%

“…The virus has been shown to infect the Italian isolates Sulfolobus solfataricus strains P1, P2 and a highly susceptible strain derived from S. solfataricus P2, termed strain P2-2-2-12. Infection of other species, including Sulfolobus islandicus, Sulfolobus acidocaldarius and Sulfolobus tokodaii, as well as other uncharacterized Sulfolobus isolates from Yellowstone National Park has been unproductive [1,2]. The full host range of the virus is not known.…”

Section: Introductionmentioning

confidence: 95%

Genetics, biochemistry and structure of the archaeal virus STIV

Fulton

Bothner

Lawrence

et al. 2009

Biochemical Society Transactions

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STIV (Sulfolobus turreted icosahedral virus) has been the subject of detailed structural, genetic, transcriptomic, proteomic and biochemical studies. STIV arguably has been investigated in more detail than any other archaeal virus. As a result, we know more about STIV than other viruses infecting members of the Archaea domain. Like most viruses isolated from crenarchaeal hosts, STIV has little in common with viruses that infect eukaryotic and bacterial hosts and should be considered the founding member of a new virus family. However, despite this lack of obvious homology with other viruses, STIV has components of gene content, replication strategy and particle structure reminiscent of viruses of the Eukarya and Bacteria domains, suggesting an evolutionary relationship between viruses from all domains of life. The present mini-review describes the current knowledge of this virus and insights it has given us into viral and cellular evolution, as well as newly developed tools for the further study of STIV-host interactions.

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Transcriptome Analysis of Infection of the Archaeon Sulfolobus solfataricus with Sulfolobus Turreted Icosahedral Virus

Cited by 24 publications

References 0 publications

40 Years of archaeal virology: Expanding viral diversity

40 Years of archaeal virology: Expanding viral diversity

Structure-Based Mutagenesis of Sulfolobus Turreted Icosahedral Virus B204 Reveals Essential Residues in the Virion-Associated DNA-Packaging ATPase

Genetics, biochemistry and structure of the archaeal virus STIV

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