Transcriptome analysis of Ehrlichia ruminantium in the ruminant host at the tick bite site and in the tick vector salivary glands

Tjale, Mabotse A.; Liebenberg, Junita; Steyn, H.C.; Kleef, Mirinda Van; Pretorius, Ashley

doi:10.1016/j.ttbdis.2020.101646

Cited by 1 publication

(2 citation statements)

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“…Previous transcriptomic studies described E . ruminantium DEGs induced during infection in vitro and SG [ 29 , 55 ] or used alternative approaches for host gene identification [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Advances in high throughput sequencing technologies have accelerated genomic research [ 26 ] and enabled the uncovering of many disease-causing pathogens in ticks [ 27 ]. High throughput transcriptomic sequencing has facilitated the determination of pathogen’s and vector tissues’ gene expression during pathogen acquisition and transmission [ 28 , 29 ]. In-depth analysis of midgut transcriptomes provides an avenue for the identifying and development of potential targets for novel tick control methods.…”

Section: Introductionmentioning

confidence: 99%

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De novo assembly and annotation of the Amblyomma hebraeum tick midgut transcriptome response to Ehrlichia ruminantium infection

Omondi,

Zweygarth,

Murungi

et al. 2023

PLoS Negl Trop Dis

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The South African bont tick Amblyomma hebraeum is a hematophagous vector for the heartwater disease pathogen Ehrlichia ruminantium in southern Africa. During feeding, the tick’s enterocytes express proteins that perform vital functions in blood digestion, including proteins that may be involved in E. ruminantium acquisition, colonization or immunity. To delineate the molecular mechanism of midgut response to E. ruminantium infection, we performed comparative analyses of midgut transcriptomes of E. ruminantium infected engorged A. hebraeum nymphs, and infected adult male and female ticks with their corresponding matched uninfected controls, before and during feeding. A total of 102,036 unigenes were annotated in public databases and their expression levels analyzed for engorged nymphs as well as unfed and partly-fed adult ticks. There were 2,025 differentially expressed genes (DEGs) in midguts, of which 1,225 unigenes were up-regulated and 800 unigenes were down-regulated in the midguts of infected ticks. Annotation of DEGs revealed an increase in metabolic and cellular processes among E. ruminantium infected ticks. Notably, among the infected ticks, there was up-regulation in the expression of genes involved in tick immunity, histone proteins and oxidative stress responses. We also observed up-regulation of glycoproteins that E. ruminantium could potentially use as docking sites for host cell entry. Insights uncovered in this study offer a platform for further investigations into the molecular interaction between E. ruminantium and A. hebraeum.

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“…Previous transcriptomic studies described E . ruminantium DEGs induced during infection in vitro and SG [ 29 , 55 ] or used alternative approaches for host gene identification [ 56 ].…”

Section: Discussionmentioning

confidence: 99%