Chemoautotrophs within Campylobacterota, especially
Sulfurovum
and
Sulfurimonas
, are abundant in the seawater–sediment interface of the Formosa cold seep in the South China Sea. However, the
in situ
activity and function of Campylobacterota are unknown. In this study, the geochemical role of Campylobacterota in the Formosa cold seep was investigated with multiple means. Two members of
Sulfurovum
and
Sulfurimonas
were isolated for the first time from deep-sea cold seep. These isolates are new chemoautotrophic species that can use molecular hydrogen as an energy source and CO
2
as a sole carbon source. Comparative genomics identified an important hydrogen-oxidizing cluster in
Sulfurovum
and
Sulfurimonas
. Metatranscriptomic analysis detected high expression of hydrogen-oxidizing gene in the RS, suggesting that H
2
was likely an energy source in the cold seep. Genomic analysis indicated that the
Sulfurovum
and
Sulfurimonas
isolates possess a truncated sulfur-oxidizing system, and metatranscriptomic analysis revealed that
Sulfurovum
and
Sulfurimonas
with this genotype were active in the surface of RS and likely contributed to thiosulfate production. Furthermore, geochemical and
in situ
analyses revealed sharply decreased nitrate concentration in the sediment–water interface due to microbial consumption. Consistently, the denitrification genes of
Sulfurimonas
and
Sulfurovum
were highly expressed, suggesting an important contribution of these bacteria to nitrogen cycling. Overall, this study demonstrated that Campylobacterota played a significant role in the cycling of nitrogen and sulfur in a deep-sea cold seep.
IMPORTANCE
Chemoautotrophs within Campylobacterota, in particular
Sulfurovum
and
Sulfurimonas
, are ubiquitous in deep-sea cold seeps and hydrothermal vents. However, to date, no
Sulfurovum
or
Sulfurimonas
has been isolated from cold seeps, and the ecological roles of these bacteria in cold seeps remain to be investigated. In this study, we obtained two isolates of
Sulfurovum
and
Sulfurimonas
from Formosa cold seep, South China Sea. Comparative genomics, metatranscriptomics, geochemical analysis, and
in situ
experimental study indicated collectively that Campylobacterota played a significant part in nitrogen and sulfur cycling in cold seep and was the cause of thiosulfate accumulation and sharp reduction of nitrate level in the sediment–water interface. The findings of this study promoted our understanding of the
in situ
function and ecological role of deep-sea Campylobacterota.