Transcriptome analysis of Corynebacterium pseudotuberculosis biovar Equi in two conditions of the environmental stress

Gomide, Anne Cybelle Pinto; Ibraim, Izabela Coimbra; Alves, Jorianne Thyeska Castro; Sá, Pablo Gomes de; Silva, Yuri Rafael de Oliveira; Santana, Mariana Passos; Silva, Wanderson Marques da; Folador, Edson Luiz; Mariano, Diego; Castro, Thiago Luiz de Paula; Barbosa, Silvanira; Dorella, Fernanda Alves; Carvalho, Alex F.; Pereira, Felipe Luiz; Leal, Carlos Augusto Gomes; Figueiredo, Henrique César Pereira; Azevedo, Vasco; Silva, Artur M. S.; Folador, Adriana Ribeiro Carneiro

doi:10.1016/j.gene.2018.08.028

Cited by 5 publications

(18 citation statements)

References 85 publications

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“…Franco et al inferred the GCNs of four C. pseudotuberculosis strains (258, T1, Cp13 and 1002) using RNA-Seq datasets [40][41][42][43]. The authors applied the following bioinformatic tools: (i) miRsig [55] to infer the GCNs of all genes and differentially expressed genes (DEGs), (ii) miRinfluence [56] to identify the predicted networks' influential and causal genes and (iii) Online GEne Essentiality (OGEE) database v2 [57] to classify the causal genes as essential, nonessential or conditionally essential [54].…”

Section: Gene Co-expression Network and Transcriptional Regulatory Nmentioning

confidence: 99%

“…During environmental changes, a reduction, or even a lack of growth, is considered normal in bacteria [40,107]. Three studies in C. pseudotuberculosis point out a reduction of replication of ~23%, ~27% and ~34% in strain 1002 and 16%, 20% and 36% in strain 258 under osmotic, thermal and acid stresses, respectively [40][41][42]. The first study performed a differential expression analysis under these conditions in C. pseudotuberculosis 1002 and identified DEGs involved in oxidoreduction, adhesion and cell division processes [40].…”

Section: Response To Osmotic Thermal and Acid Stressmentioning

confidence: 99%

“…The first study performed a differential expression analysis under these conditions in C. pseudotuberculosis 1002 and identified DEGs involved in oxidoreduction, adhesion and cell division processes [40]. The other two allow us to further understand the transcriptional response induced during these stresses in C. pseudotuberculosis 258 [41,42]. In all three studies, the authors performed transcriptome analyses using the SOLiD 3 Plus platform [40][41][42].…”

Section: Response To Osmotic Thermal and Acid Stressmentioning

confidence: 99%

“…The other two allow us to further understand the transcriptional response induced during these stresses in C. pseudotuberculosis 258 [41,42]. In all three studies, the authors performed transcriptome analyses using the SOLiD 3 Plus platform [40][41][42]. A notable TF identified in these two strains is TetR2, a DEG found across the three stress conditions.…”

Section: Response To Osmotic Thermal and Acid Stressmentioning

confidence: 99%

“…Despite the importance of C. pseudotuberculosis, little is known about the mechanisms that control gene expression. A few studies have evaluated its transcriptional profile across different environmental conditions [40][41][42][43] and a few others have investigated mutant strains showing the importance of specific genes, such as plD [44], the fagABC operon [45] and phoP [46] in C. pseudotuberculosis virulence and pathogenicity. Recently, Parise et al reconstructed in silico TRNs for all C. pseudotuberculosis strains with complete genome sequences and made it available in the seventh version of CoryneRegNet [22].…”

Section: Introductionmentioning

confidence: 99%

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The Transcriptional Regulatory Network of Corynebacterium pseudotuberculosis

Parise

Gomide

et al. 2021

Microorganisms

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Corynebacterium pseudotuberculosis is a Gram-positive, facultative intracellular, pathogenic bacterium that infects several different hosts, yielding serious economic losses in livestock farming. It causes several diseases including oedematous skin disease (OSD) in buffaloes, ulcerative lymphangitis (UL) in horses, and caseous lymphadenitis (CLA) in sheep, goats and humans. Despite its economic and medical-veterinary importance, our understanding concerning this organism’s transcriptional regulatory mechanisms is still limited. Here, we review the state of the art knowledge on transcriptional regulatory mechanisms of this pathogenic species, covering regulatory interactions mediated by two-component systems, transcription factors and sigma factors. Key transcriptional regulatory players involved in virulence and pathogenicity of C. pseudotuberculosis, such as the PhoPR system and DtxR, are in the focus of this review, as these regulators are promising targets for future vaccine design and drug development. We conclude that more experimental studies are needed to further understand the regulatory repertoire of this important zoonotic pathogen, and that regulators are promising targets for future vaccine design and drug development.

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Section: Gene Co-expression Network and Transcriptional Regulatory Nmentioning

confidence: 99%

Section: Response To Osmotic Thermal and Acid Stressmentioning

confidence: 99%

Section: Response To Osmotic Thermal and Acid Stressmentioning

confidence: 99%

Section: Response To Osmotic Thermal and Acid Stressmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Transcriptional Regulatory Network of Corynebacterium pseudotuberculosis

Parise

Gomide

et al. 2021

Microorganisms

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Prediction of new vaccine targets in the core genome of Corynebacterium pseudotuberculosis through omics approaches and reverse vaccinology

Araújo

Alves

Nogueira

et al. 2019

Gene

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Açlık Stresine Maruz Kalan Corynebacterium pseudotuberculosis’in SDS-PAGE İle Proteinlerinin Analizi

Demir,

Erdenlig Gürbilek

2023

Harran Üniversitesi Veteriner Fakültesi Dergisi

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Corynebacterium pseudotuberculosis, hücre içi bir patojen olup küçük ruminantlarda kazeöz lenfadenit (KLA) hastalığına sebep olan, Gram-pozitif fakültatif bir bakteridir. Corynebacterium pseudotuberculosis, insanlarda ve hayvanlarda hastalık oluşturan bir bakteri türüdür. Bu bakteri, açlık stresine maruz kaldığında, hücresel yapısını ve metabolizmasını değiştirerek adaptasyon gösterir. Bu değişiklikler, bakterinin protein ekspresyonunda da değişikliklere neden olabilir. Bu çalışmada da, Corynebacterium pseudotuberculosis’in stres faktörlerine maruz bırakıldığında ve genel besiyerlerinde üretildiğinde protein profillerinde nasıl bir farklılık olacağı araştırılmıştır. SDS-PAGE analizi, açlık stresine maruz kalan Corynebacterium pseudotuberculosis'teki protein ekspresyonundaki değişiklikleri incelemek için kullanılabilir. Bu analiz, bakterinin strese karşı adaptasyonunu ve bu adaptasyonun enfeksiyonun yayılmasına nasıl katkıda bulunduğunu daha iyi anlamamıza yardımcı olabilir. Bu araştırmada SDS-PAGE analizi ile izole edilen ve referans olarak kullanılan Corynebacterium pseudotuberculosis suşlarının Tryptic Soy Broth ve RPMI 1640 (Roswell Park Memorial Institute) besiyerlerinde üretilip strese maruz bırakılan suşun protein profilleri incelenmiştir. Sonuç olarak, strese maruz bırakılan Corynebacterium suşları arasında protein yapıları arasında profil farklılıkları olduğu tespit edilmiştir.

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Transcriptome analysis of Corynebacterium pseudotuberculosis biovar Equi in two conditions of the environmental stress

Cited by 5 publications

References 85 publications

The Transcriptional Regulatory Network of Corynebacterium pseudotuberculosis

The Transcriptional Regulatory Network of Corynebacterium pseudotuberculosis

Prediction of new vaccine targets in the core genome of Corynebacterium pseudotuberculosis through omics approaches and reverse vaccinology

Açlık Stresine Maruz Kalan Corynebacterium pseudotuberculosis’in SDS-PAGE İle Proteinlerinin Analizi

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