Transcriptome Analysis of Chlorantraniliprole Resistance Development in the Diamondback Moth Plutella xylostella

Lin, Qimei; Jin, Fengliang; Hu, Zhongying; Chen, Huanyu; Yin, Fei; Li, Zhenyu; Dong, Xiaolin; Zhang, Deyong; Ren, Sumei; Feng, Xiaoqin

doi:10.1371/journal.pone.0072314

Cited by 77 publications

(81 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, a lack of genetic information is still a barrier to our knowledge of this insect pest. NGS, developed for rapid sequencing and transcriptomics research, has enabled the use of RNA-Seq and DGE methods to analyze the ACB transcriptome and insecticide mechanisms [14, 15]. …”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

et al. 2017

View full text Add to dashboard Cite

BackgroundThe Asian corn borer (ACB), Ostrinia furnacalis (Guenée), has become the most damaging insect pest of corn in Asia. However, the lack of genome or transcriptome information heavily hinders our further understanding of ACB in every aspect at a molecular level and on a genome-wide scale. Here, we used the Ion Torrent Personal Genome Machine (PGM) Sequencer to explore the ACB transcriptome and to identify relevant genes in response to flubendiamide, showing high selective activity against ACB.ResultsWe obtained 35,430 unigenes, with an average length of 716 bp, representing a dramatic expansion of existing cDNA sequences available for ACB. These sequences were annotated with Non-redundant Protein (Nr), Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to better understand their functions. A total of 31 cytochrome P450 monooxygenases (P450s), 27 carboxyl/cholinesterases (CCEs) and 19 glutathione S-transferases (GSTs) were manually curated to construct phylogenetic trees, and 25 unigenes encoding target proteins (acetylcholinesterase, nicotinic acetylcholine receptor, gamma-aminobutyric acid receptor, glutamate-gated chloride channel, voltage-gated sodium channel and ryanodine receptor) were identified. In addition, we compared and validated the differentially expressed unigenes upon flubendiamide treatment, revealing that the genes for detoxification enzymes (P450s and esterase), calcium signaling pathways and muscle control pathways (twitchin and tropomyosin), immunoglobulin (hemolin), chemosensory protein and heat shock protein 70 were significantly overexpressed in response to flubendiamide, while the genes for cuticular protein, protease and oxidoreductase showed much lower expression levels.ConclusionThe obtained transcriptome information provides large genomic resources available for further studies of ACB. The differentially expressed gene data will elucidate the molecular mechanisms of ACB in response to the novel diamide insecticide, flubendiamide. In particular, these findings will facilitate the identification of the genes involved in insecticide resistance and the development of new compounds to control the ACB.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3431-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In a chlorantraniliprole-resistant DBM population from Yunnan, 20% of individuals were found to have G4946E mutations, and synergy between this and three other point mutations (E1338D, Q4594L, and I4790M) caused the high level of resistance to this insecticide (30). Lin et al (52) investigated transcriptome assembly and used a tag-based digital gene expression (DGE) system to analyze differences between DBM populations exhibiting low, moderate, and high levels of resistance to chlorantraniliprole. Genes related to insecticide resistance [e.g., those coding for cytochrome P450, glutathione-S-transferase, ryanodine receptor(s), and connectin] had distinctive expression profiles in the DGE libraries generated for the various chlorantraniliprole-resistant DBM populations, indicating that multiple different resistance mechanisms are represented in these populations (52).…”

Section: Molecular Basis Of Insecticide Resistancementioning

confidence: 99%

Biology, Ecology, and Management of the Diamondback Moth in China

Feng

Liu

et al. 2016

Annu. Rev. Entomol.

Self Cite

187

205

View full text Add to dashboard Cite

The diamondback moth (DBM), Plutella xylostella (L.), costs the Chinese economy US$0.77 billion annually, and considerable research has focused on its biology, ecology, and management. Much of this research has been published locally and is inaccessible outside China. Since 1990 Brassica vegetable production has increased 20-fold and production practices have intensified, but losses continue to increase. Insecticide use is widespread and many DBM populations, particularly in southern provinces, are resistant to multiple compounds. The molecular bases of several insecticide resistance mechanisms are well understood, and genetic studies suggest that insecticide-resistant populations migrate northward in spring and that back migrations may occur in southern provinces. Fundamental studies have improved our understanding of the effects of temperature on DBM population dynamics and distributions and of interactions between DBM and its well-established parasitoid fauna. Nationally coordinated research is developing regional management strategies that integrate locally appropriate biological, physical, cultural, and insecticidal control, but sustaining their adoption will prove an enormous challenge.

show abstract

“…Using next generation sequencing, Lin et al 22 compared differential gene expression between three DBM strains with low, moderate and high levels of resistance to chlorantraniliprole. Compared to a susceptible reference strain, 189 unigenes were overexpressed, 19 of which were P450 metabolic detoxification enzymes.…”

mentioning

confidence: 99%

Characterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.)

Tang

et al. 2015

Sci Rep

View full text Add to dashboard Cite

Cytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xylostella P450 genes from clans 3 and 4. Gene expression of cytochrome P450s was quantified across multiple developmental stages (egg, larva, pupa and adult) and tissues (head and midgut) using P. xylostella strains susceptible or resistant to insecticides chlorpyrifos and fiprinol. Expression of the lepidopteran specific CYP367s predominantly occurred in head tissue suggesting a role in either olfaction or detoxification. CYP340s with abundant transposable elements and relatively high expression in the midgut probably contribute to the detoxification of insecticides or plant toxins in P. xylostella. This study will facilitate future functional studies of the P. xylostella P450s in detoxification.

show abstract

Transcriptome Analysis of Chlorantraniliprole Resistance Development in the Diamondback Moth Plutella xylostella

Cited by 77 publications

References 36 publications

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

Biology, Ecology, and Management of the Diamondback Moth in China

Characterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.)

Contact Info

Product

Resources

About