Transcriptome analysis of blood and spleen in virulent and avirulent mouse malaria infection

Zhao, Yuancun; Hosking, Caroline; Cunningham, Deirdre; Langhorne, Jean; Lin, Jing-wen

doi:10.1038/s41597-020-00592-1

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…In this work, tamoxifen further increased the splenic index, particularly on day eight post-infection. This finding could be explained by the fact that by increasing parasitaemia, tamoxifen could increase antigenic stimulation in the spleen, promoting inflammation and cell recruitment in the spleen, as described by Del Portillo et al and Zhao, et al [39,40].…”

Section: Discussionmentioning

confidence: 88%

Tamoxifen Suppresses the Immune Response to Plasmodium berghei ANKA and Exacerbates Symptomatology

et al. 2021

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Malaria is the most lethal parasitic disease in the world. Mortality and severity in symptoms are higher in men than women, suggesting that oestrogens, which are in higher concentration in females than in males, may regulate the immune response against malaria. Tamoxifen, a selective oestrogen receptor modulator used in breast cancer treatment due to its antagonistic effect on oestrogen receptors α and β, is also studied because of its potential therapeutic use for several parasitic diseases. However, most studies, including one in malaria, have not addressed the immunomodulatory role of tamoxifen. In this work, we evaluated the effect of tamoxifen on the immune response of CBA/Ca mice against Plasmodium berghei ANKA. This study showed for the first time that tamoxifen increased parasite load, aggravated symptoms by decreasing body temperature and body weight, and worsened anaemia. Additionally, tamoxifen significantly increased the splenic index and the percentages of CD4+ and NK+ cells on day eight post-infection. By contrast, tamoxifen decreased both CD8+ and B220+ populations in the spleen and decreased the serum levels of IL-2, IL-6, and IL-17. Our findings support the notion that tamoxifen is a potent immunomodulator in malaria-infected mice and suggest caution when administering it to malaria-infected women with breast cancer.

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Section: Discussionmentioning

confidence: 88%

Tamoxifen Suppresses the Immune Response to Plasmodium berghei ANKA and Exacerbates Symptomatology

et al. 2021

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“…We did not test the SNPs of K13 using this microarray because K13 was not identified when the chip was developed. Although this custom array is not available now, some new microarrays have been developed, for example, Illumina Bead Chip microarray, protein array, or peptide array, which provide a high throughout and a powerful platform in malaria field ( Bailey et al, 2020 ; Zhao et al, 2020 ).…”

Section: Limitationsmentioning

confidence: 99%

Genomic Epidemiology of Antimalarial Drug Resistance in Plasmodium falciparum in Southern China

Huang

Jacob

Takala-Harrison

et al. 2021

Front. Cell. Infect. Microbiol.

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Emerging artemisinin resistance in Southeast Asia poses a significant risk to malaria control and eradication goals, including China’s plan to eliminate malaria nationwide by 2020. Plasmodium falciparum was endemic in China, especially in Southern China. Parasites from this region have shown decreased susceptibility to artemisinin and delayed parasite clearance after artemisinin treatment. Understanding the genetic basis of artemisinin resistance and identifying specific genetic loci associated with this phenotype is crucial for surveillance and containment of resistance. In this study, parasites were collected from clinical patients from Yunnan province and Hainan island. The parasites were genotyped using a P. falciparum-specific single nucleotide polymorphism (SNP) microarray. The SNP profiles examined included a total of 27 validated and candidate molecular markers of drug resistance. The structure of the parasite population was evaluated by principal component analysis by using the EIGENSOFT program, and ADMIXTURE was used to calculate maximum likelihood estimates for the substructure analysis. Parasites showed a high prevalence of resistance haplotypes of pfdhfr and pfdhps and moderate prevalence of pfcrt. There was no mutation identified on pfmdr1. Candidate SNPs on chromosomes 10, 13, and 14 that were associated with delayed parasite clearance showed a low prevalence of mutants. Parasites from Southern China were clustered and separated from those from Southeast Asia. Parasites from Yunnan province were substructured from parasites from Hainan island. This study provides evidence for a genomic population with drug resistance in Southern China and also illustrates the utility of SNP microarrays for large-scale parasite molecular epidemiology.

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Mouse Models for Unravelling Immunology of Blood Stage Malaria

et al. 2022

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Malaria comprises a spectrum of disease syndromes and the immune system is a major participant in malarial disease. This is particularly true in relation to the immune responses elicited against blood stages of Plasmodium-parasites that are responsible for the pathogenesis of infection. Mouse models of malaria are commonly used to dissect the immune mechanisms underlying disease. While no single mouse model of Plasmodium infection completely recapitulates all the features of malaria in humans, collectively the existing models are invaluable for defining the events that lead to the immunopathogenesis of malaria. Here we review the different mouse models of Plasmodium infection that are available, and highlight some of the main contributions these models have made with regards to identifying immune mechanisms of parasite control and the immunopathogenesis of malaria.

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Transcriptome analysis of blood and spleen in virulent and avirulent mouse malaria infection

Cited by 3 publications

References 19 publications

Tamoxifen Suppresses the Immune Response to Plasmodium berghei ANKA and Exacerbates Symptomatology

Tamoxifen Suppresses the Immune Response to Plasmodium berghei ANKA and Exacerbates Symptomatology

Genomic Epidemiology of Antimalarial Drug Resistance in Plasmodium falciparum in Southern China

Mouse Models for Unravelling Immunology of Blood Stage Malaria

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