Transcriptome analysis of acerola fruit ripening: insights into ascorbate, ethylene, respiration, and softening metabolisms

Santos, Clesivan Pereira dos; Batista, Mathias Coelho; Saraiva, Kátia Daniella da Cruz; Roque, André Luiz Maia; Miranda, Rafael de Souza; Silva, Lorena; Moura, Carlos Farley Herbster; Filho, Elenilson G. Alves; Canuto, Kirley Marques; Costa, José Hélio

doi:10.1007/s11103-019-00903-0

Cited by 29 publications

(14 citation statements)

References 164 publications

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“…In this regard, a transcriptome analysis conducted in banana and acerola indicated that PME, PG, EXP, PL, and XTH levels increase significantly during softening in postharvest storage. 31,32 A previous study reported that increased expression of MdPG1 in apple during storage exhibited a strong correlation with softening. 33 Downregulation of PL in tomato was associated with increased fruit firmness and reduced postharvest fruit softening.…”

Section: Discussionmentioning

confidence: 98%

The MdXTHB gene is involved in fruit softening in ‘Golden Del. Reinders’ (Malus pumila)

Yang

Cheng

et al. 2020

J Sci Food Agric

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BACKGROUND: Fruit softening is a major determinant of commercial value and shelf life. A transcriptomic analysis of 'Golden Delicious' and 'Golden Del. Reinders' (a bud mutation of 'Golden Delicious' that readily softens) apple fruit was conducted during storage. RESULTS: A comparative analysis of the obtained expression profiles of fruit between two cultivars identified 1345 upregulated and 3475 downregulated differentially expressed genes (DEGs). The DEGs identified were associated with cellular processes and carbohydrate metabolism and were especially enriched in cell-wall-related genes. Among the cell-wall-related genes, the xyloglucan endotransglucosylase/hydrolases (XTH) gene MdXTHB was significantly upregulated and exhibited high expression levels in 'Golden Del. Reinders' fruit, which had a lower level of firmness relative to 'Golden Delicious'. Overexpression of MdXTHB in both 'Golden Delicious' and 'Fuji', which typically maintain high levels of firmness in storage, exhibited faster rates of softening and an earlier peak of ethylene production than empty-vector-infiltrated fruit did. CONCLUSION: The results of this study indicate that MdXTHB potentially promotes apple fruit softening by degrading the fruit cell wall. This result is also useful to designing further experiments on the molecular regulation of fruit softening in apple. Supporting information may be found in the online version of this article.

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Section: Discussionmentioning

confidence: 98%

The MdXTHB gene is involved in fruit softening in ‘Golden Del. Reinders’ (Malus pumila)

Yang

Cheng

et al. 2020

J Sci Food Agric

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“…Fruits in particular contain large amounts of Asc, which is the best source of vitamin C for animals. Tree transcriptome sequencing revealed that the expression of genes involved in the L-galactose pathway is linked to high Asc levels in acerola fruit [87]. Large-sized mandarin fruits contain high AsA levels [88], whereas fruit color and fruit size had no detected effect on the Asc content in sweet cherry fruits [89].…”

Section: Fruit Developmentmentioning

confidence: 99%

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

et al. 2019

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Reactive oxygen species (ROS) are constantly produced by metabolically active plant cells. The concentration of ROS may determine their role, e.g., they may participate in signal transduction or cause oxidative damage to various cellular components. To ensure cellular homeostasis and minimize the negative effects of excess ROS, plant cells have evolved a complex antioxidant system, which includes ascorbic acid (AsA). AsA is a multifunctional metabolite with strong reducing properties that allows the neutralization of ROS and the reduction of molecules oxidized by ROS in cooperation with glutathione in the Foyer-Halliwell-Asada cycle. Antioxidant enzymes involved in AsA oxidation and reduction switches evolved uniquely in plants. Most experiments concerning the role of AsA have been performed on herbaceous plants. In addition to extending our understanding of this role in additional taxa, fundamental knowledge of the complex life cycle stages of woody plants, including their development and response to environmental factors, will enhance their breeding and amend their protection. Thus, the role of AsA in woody plants compared to that in nonwoody plants is the focus of this paper. The role of AsA in woody plants has been studied for nearly 20 years. Studies have demonstrated that AsA is important for the growth and development of woody plants. Substantial changes in AsA levels, as well as reduction and oxidation switches, have been reported in various physiological processes and transitions described mainly in leaves, fruits, buds, and seeds. Evidently, AsA exhibits a dual role in the photoprotection of the photosynthetic apparatus in woody plants, which are the most important scavengers of ozone. AsA is associated with proper seed production and, thus, woody plant reproduction. Similarly, an important function of AsA is described under drought, salinity, temperature, light stress, and biotic stress. This report emphasizes the involvement of AsA in the ecological advantages, such as nutrition recycling due to leaf senescence, of trees and shrubs compared to nonwoody plants.

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“…Respiration is the primary catabolic process that contributes to subsequent degradation, senescence, and natural ripening. Minimizing mechanical injuries, harvesting at optimum maturity through adequate sanitary procedures, and supplying the optimal humidity and temperature throughout all marketing steps are the main components in increasing the postharvest life of fresh vegetables and fruits and preserving quality (Bruinsma & Paull, 1984;Santos et al, 2019;Kochevenko et al, 2012). Additional variables involve changing the levels of ethylene (C 2 H 4 ), carbon dioxide (CO 2 ), and oxygen (O 2 ) in the environment surrounding the product to levels not found in the air (Flores-Cortez et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Fruit preservation packaging technology based on air adjustment packaging method

Anggono¹,

Rebezov

Mironov

et al. 2022

Food Sci. Technol

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Fresh vegetables and fruits need oxygen (O 2 ) to carry out their metabolic activities, particularly respiration. The procedure where the actively respiring commodity is sealed in film packages made of polymer to change the CO 2 and O 2 levels of concentration inside the package environment required to increase shelf-life and preserve freshness is referred to as modified atmosphere packaging (MAP). To affect the product's metabolism being packaged or the activity of organisms that cause degradation to extend the time of preservation, it is frequently desired to create an environment high in CO 2 and low in O 2 . MAP changes the environment and increases moisture preservation that has a bigger impact on quality preservation. Moreover, packing separates the product from the surrounding environment, assisting in the creation of circumstances that, if not hygienic, at the very least minimize exposure to infections and pollutants, as well as physiological damage. MAP is a dynamic mechanism that occurs concurrently throughout permeation and respiration. As a result, MAP design necessitates the assessment of the product's intrinsic features, such as film permeability, optimal O 2 and CO 2 gas concentrations, and respiration rate. The goal of MAP design is to specify parameters that will provide the greatest feasible environment within the package for increasing the product's shelf-life in the quickest possible time. This is accomplished by synchronizing the packed produce's respiration rate with O2 and CO2 gas penetration rate through the film. The current study contains a detailed discussion of all of these elements of MAP.

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Transcriptome analysis of acerola fruit ripening: insights into ascorbate, ethylene, respiration, and softening metabolisms

Cited by 29 publications

References 164 publications

The MdXTHB gene is involved in fruit softening in ‘Golden Del. Reinders’ (Malus pumila)

The MdXTHB gene is involved in fruit softening in ‘Golden Del. Reinders’ (Malus pumila)

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

Fruit preservation packaging technology based on air adjustment packaging method

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