Transcriptome analysis in the skin of Carassius auratus challenged with Aeromonas hydrophila

Wang, Ruixia; Hu, Xiucai; Lü, Aijun; Liu, Rongrong; Sun, Jingfeng; Sung, Yeong Yik; Song, Yuegang

doi:10.1016/j.fsi.2019.09.039

Cited by 29 publications

(21 citation statements)

References 43 publications

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“…In the present study, the indicating that the pathogenic A. hydrophila infected-fish displayed obviously clinical symptoms during early infection at about 12 hpi, which then was quickly developed a typical signs of this disease. The pathological changes were basically consistent with inflammatory cell infiltration, hemorrhage and necrosis accorded with previous A. hydrophila infection reports [ 27 , [30] , [36] ]. Recently, the transcriptomics analysis in various organs (eg., kidney, skin and pharyngeal tissue) of C. auratus infected with different pathogens were successively reported [ [30] , [37] , [38] ].…”

Section: Discussionsupporting

confidence: 91%

“…Fishes were challenged with A. hydrophila by immersion as our previously reported by Wang et al. [ 30 ], and fed with commercial dried pelleted feed (Tongwei, China) twice daily. Briefly, sixty fish were randomly chosen and averaged to two groups namely the control group and infected group, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Although C. auratus is highly susceptible to bacterial pathogenes, the exploration of immunity at the molecular level is little investigated by multi-omic approaches [ 28 ]. Recently, our group studies have reported the gene differential expression profiling in skin immune response of C. auratus during A. hydrophila infection [ 29 , 30 ]. However, the mucosal immune mechanism of gills at transcriptomic and proteomic levels in fish against A. hydrophila infection, are still poorly understood so far.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comprehensive transcriptomics and proteomics analysis of Carassius auratus gills in response to Aeromonas hydrophila

Bai

Liu

et al. 2023

Fish and Shellfish Immunology Reports

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Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive transcriptomics and proteomics analysis of Carassius auratus gills in response to Aeromonas hydrophila

Bai

Liu

et al. 2023

Fish and Shellfish Immunology Reports

Self Cite

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“…The hierarchical analysis showed that all the samples associated with a given experiential group were clustered together, reflecting the comparable gene expression profiles of samples in each group. An RNA-Seq based study identified 8580 differentially expressed genes in the skin of Aeromonas hydrophila -infected goldfish ( Carassius auratus ) at 12 h post-infection 31 . Likewise, our global gene expression results showed a large number of DETs in Atlantic salmon skin infected with M. viscosa .…”

Section: Discussionmentioning

confidence: 99%

Global gene expression responses of Atlantic salmon skin to Moritella viscosa

Eslamloo

Kumar

Xue

et al. 2022

Sci Rep

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Moritella viscosa is a Gram-negative pathogen that causes large, chronic ulcers, known as winter-ulcer disease, in the skin of several fish species including Atlantic salmon. We used a bath challenge approach to profile the transcriptome responses of M. viscosa-infected Atlantic salmon skin at the lesion (Mv-At) and away from the lesion (Mv-Aw) sites. M. viscosa infection was confirmed through RNA-based qPCR assays. RNA-Seq identified 5212 and 2911 transcripts differentially expressed in the Mv-At compared to no-infection control and Mv-Aw groups, respectively. Also, there were 563 differentially expressed transcripts when comparing the Mv-Aw to control samples. Our results suggest that M. viscosa caused massive and strong, but largely infection site-focused, transcriptome dysregulations in Atlantic salmon skin, and its effects beyond the skin lesion site were comparably subtle. The M. viscosa-induced transcripts of Atlantic salmon were mainly involved in innate and adaptive immune response-related pathways, whereas the suppressed transcripts by this pathogen were largely connected to developmental and cellular processes. As validated by qPCR, M. viscosa dysregulated transcripts encoding receptors, signal transducers, transcription factors and immune effectors playing roles in TLR- and IFN-dependent pathways as well as immunoregulation, antigen presentation and T-cell development. This study broadened the current understanding of molecular pathways underlying M. viscosa-triggered responses of Atlantic salmon, and identified biomarkers that may assist to diagnose and combat this pathogen.

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“…By the quick advancement of highthroughput sequencing technologies, it provides valuable information for understanding host-pathogen interactions, including gene expression and immune responses [14]. By using high-throughput sequencing technologies, it have been reported that numerous of conserved and novel genes involved in innate and adaptive immunities during viral and bacterial infections in many shes such as Oncorhynchus mykiss [15], Oncorhynchus nerka [16], Schizothorax Prenanti [17], Oreochromis niloticus [18], Carassius Auratus [19], Hybrid Sturgeon [20], Common Carp [21], and Ctenopharyngodon idellus [22]. Research on transcriptomic and proteomic of O. mykiss has indicated that RBCs possessing nucleus support many functions of immunity, e.g.…”

Section: Introductionmentioning

confidence: 99%

Aeromonas Hydrophila Infection Activates Death Receptor Apoptosis Pathway in the RBCs of Grass Carp (Ctenopharyngodon idellus)

Yang

Zhang

et al. 2020

Preprint

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Background: Fish blood contains RBCs (Red blood cells) as the major cells. Unlike the absence of nucleus in mammalian RBC, fish RBC contains a nucleus. Previous researches have indicated that fish RBCs have a significant function in immune responses. However, the mechanism underlying the immune responses against bacterial infection of teleost RBCs remains enigmatic. To decipher the mechanisms, after the infection of A. hydrophila, transcriptomic profiling of grass carp RBCs was analyzed. Results: Outcomes demonstrated that there were 2144 altogether DEGs (Differently Expressed Genes) between the A. hydrophila non-disease groups, which includes 817 up-regulated and 1327 down-regulated DEGs. Differently Expressed Genes were allocated to 45 GO terms, including 20 natural procedure terms, 14 cell related element, and 11 terms related to molecular functions. Likewise, the expression levels of cytokines by Quantitative Reverse Transcription Polymerase Chain Reaction showed that they (e.g. CCL4, CCL11, CCL20, IL-4, IL-12, and IFNα) were significantly increased after A. hydrophila infection, which was identical to the expression patterns of transcriptomic data. The infection could cause the apoptosis of RBCs which was confirmed by annexin V/PI assay. To further elucidate the apoptosis pathway, the expression levels of genes by mRNA included in cellular apoptosis were monitored. The outcomes indicated that the expressions of mRNA of p53, Fas, Leucine-rich repeats, death domain-containing (LRDD) and caspase 8 were all significantly increased, respectively. Conclusions: Our results support the notion that A. hydrophila infection could activate Fas-related death receptor apoptosis pathway in grass carp RBCs, which will reveal another insight on the mechanisms underlying the antibacterial immunity of teleost fish RBCs.

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Transcriptome analysis in the skin of Carassius auratus challenged with Aeromonas hydrophila

Cited by 29 publications

References 43 publications

Comprehensive transcriptomics and proteomics analysis of Carassius auratus gills in response to Aeromonas hydrophila

Comprehensive transcriptomics and proteomics analysis of Carassius auratus gills in response to Aeromonas hydrophila

Global gene expression responses of Atlantic salmon skin to Moritella viscosa

Aeromonas Hydrophila Infection Activates Death Receptor Apoptosis Pathway in the RBCs of Grass Carp (Ctenopharyngodon idellus)

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