Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure

Artal, Mariana Coletty; Pereira, Karina Danielle; Luchessi, Augusto Ducati; Okura, Vagner Katsumi; Henry, Theodore B.; Marques-Souza, Henrique; Umbuzeiro, Gisela de Aragão

doi:10.1016/j.envpol.2020.113963

Cited by 15 publications

(7 citation statements)

References 41 publications

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“…pesticide diflubenzuron and silver nanoparticles in this system (Artal et al 2018, Artal et al 2020, Diehl et al 2021.…”

Section: Major Interests and Research Questionsmentioning

confidence: 92%

“…Expression and chromatin profiling: Genome-wide expression profiling by RNAseq has been applied in a number of projects to identify transcriptional dynamics and responses to treatments in Parhyale, including the timecourses of embryonic development (D. Sun, H. Bruce and N. Patel, unpublished) and limb development and regeneration (C. Sinigaglia, M. Paris and M. Averof, unpublished), the study of circadian rhythms (Hunt et al 2019), the maternal to zygotic transition (S. Rawas, W. Wei, M. Jara Espejo and A. Aboobaker, unpublished), innate immune responses to bacterial infection (L. Li and A. Aboobaker, unpublished), and responses to environmental toxicants (Artal et al 2020). Single-cell or single-nucleus RNAseq has been applied to embryos (L. Blondel and C. Extavour, unpublished) Besides single-cell sequencing, cell-or tissue-specific transcriptional profiling can be carried out with the thiouracil/TU tagging approach, whereby transcripts expressed in specific cells over a chosen period of time can be tagged, isolated and sequenced (Miller et al 2009, Gay et al 2014).…”

Section: Experimental Tools Approaches and Resourcesmentioning

confidence: 99%

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The Crustacean Model <i>Parhyale hawaiensis</i>

Paris¹,

Wolff²,

Patel³

et al. 2021

Preprint

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Arthropods are the most abundant and diverse animals on earth. Among them, pancrustaceans are an ancient and morphologically diverse group, comprising a wide range of aquatic and semi-aquatic crustaceans as well as the insects, which emerged from crustacean ancestors to colonise most terrestrial habitats. Within insects, Drosophila stands out as one of the most powerful animal models, making major contributions to our understanding of development, physiology and behaviour. Given these attributes, crustaceans provide a fertile ground for exploring biological diversity through comparative studies. However, beyond insects, few crustaceans are developed sufficiently as experimental models to enable such studies. The marine amphipod Parhyale hawaiensis is currently the best established crustacean system, offering year-round accessibility to developmental stages, transgenic tools, genomic resources, and established genetics and imaging approaches. The Parhyale research community is small but diverse, investigating the evolution of development, regeneration, aspects of sensory biology, chronobiology, bioprocessing and ecotoxicology.

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“…pesticide diflubenzuron and silver nanoparticles in this system (Artal et al 2018, Artal et al 2020, Diehl et al 2021.…”

Section: Major Interests and Research Questionsmentioning

confidence: 92%

Section: Experimental Tools Approaches and Resourcesmentioning

confidence: 99%

The Crustacean Model <i>Parhyale hawaiensis</i>

Paris¹,

Wolff²,

Patel³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Parhyale are emerging as an attractive ecotoxicity test organism for coastal marine ecosystems, due to their worldwide distribution and abundance in tropical coastal and estuarine habitats, and their robust year-round reproduction in their natural habitats and in the laboratory (Alegretti et al 2016, Artal et al 2018. Recent studies have tested the effects of ammonia, metals, the pesticide diflubenzuron and silver nanoparticles in this system (Artal et al 2018, Artal et al 2020, Diehl et al 2021.…”

Section: Major Interests and Research Questionsmentioning

confidence: 99%

“…Expression and chromatin profiling: Genome-wide expression profiling by RNAseq has been applied in a number of projects to identify transcriptional dynamics and responses to treatments in Parhyale, including the timecourses of embryonic development (D. Sun, H. Bruce and N. Patel, unpublished) and limb development and regeneration (C. Sinigaglia, M. Paris and M. Averof, unpublished), the study of circadian rhythms (Hunt et al 2019), the maternal to zygotic transition (S. Rawas, W. Wei, M. Jara Espejo and A. Aboobaker, unpublished), innate immune responses to bacterial infection (L. Li and A. Aboobaker, unpublished), and responses to environmental toxicants (Artal et al 2020). Single-cell or single-nucleus RNAseq has been applied to embryos (L. Blondel and C. Extavour, unpublished) and adult limbs (A. Almazan, M. Paris and M. Averof, unpublished).…”

Section: Experimental Tools Approaches and Resourcesmentioning

confidence: 99%

The crustacean model Parhyale hawaiensis

Paris

Wolff

Patel

et al. 2022

Current Topics in Developmental Biology

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“…Additionally, other studies with marine invertebrates have also evidenced stronger immune responses in females than males (Nguyen et al, 2018, and references therein), although exceptions also exist (Jiang et al, 2017). Indeed, focusing on gene expression responses, more recent studies have identified patterns of sex-specific differentially expressed genes in different marine invertebrates (e.g., Li et al, 2019;Artal et al, 2020;Kadiene et al, 2020), which are also present in somatic tissues (e.g., Rotllant et al, 2017). Hence, it is crucial to understand the sex-specific expression of A. lixula coelomocytes, as different adaptation performance, responses to environmental stressors, and resistance against pathogens of males and females may lead to different outcomes in the ongoing expansion of this species in the Mediterranean Sea.…”

Section: Introductionmentioning

confidence: 99%

Sex-Specific Transcriptomic Differences in the Immune Cells of a Key Atlantic-Mediterranean Sea Urchin

Pérez‐Portela

Leiva

2022

Front. Mar. Sci.

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The abundance of the black sea urchin, Arbacia lixula, has been increasing during the last decades likely related to global warming. This thermophilous species has a leading role in maintaining marine barrens in the Mediterranean with the consequent negative impact on coastal rocky ecosystems due to its grazing activity. In this study, we used transcriptomic data from coelomocytes (the cell effectors of the immune system) of females and males of this sea urchin to study potential differences in performance between sexes under laboratory conditions. Differential adaptations, responses to environmental stressors, and resistance against pathogens between sexes may lead to different outcomes in the ongoing expansion of this species in the Mediterranean Sea. Differential expression analyses demonstrated the existence of 120 transcripts, corresponding to 119 genes and two isoforms of the same gene, differentially expressed between coelomocytes of females and males, being 73 up-regulated in males and 47 up-regulated in females. The differential expression patterns were retrieved from a diversity of genes that play different roles related to the immune response due to their antibacterial activity, immune cell activation, cell to cell interaction, intracellular signaling, and detoxification functioning, among others. Our results point out a higher energetic demand of male coelomocytes due to a higher immune activity than females, whereas females have more efficient molecular systems to avoid oxidative stress caused by infections. In conclusion, our study provides evidence of sex-based differences in the expression of genes related to the immune and stress responses in coelomocytes of the sea urchin A. lixula.

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Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure

Cited by 15 publications

References 41 publications

The Crustacean Model <i>Parhyale hawaiensis</i>

The Crustacean Model <i>Parhyale hawaiensis</i>

The crustacean model Parhyale hawaiensis

Sex-Specific Transcriptomic Differences in the Immune Cells of a Key Atlantic-Mediterranean Sea Urchin

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