Transcriptome analysis and transcription factors responsive to drought stress in Hibiscus cannabinus

Abstract

Kenaf is an annual bast fiber crop. Drought stress influences the growth of kenaf stems and causes a marked decrease in fiber yield and quality. Research on the drought resistance of kenaf is therefore important, but limited information is available on the response mechanism of kenaf to drought stress. In this study, a transcriptome analysis of genes associated with the drought stress response in kenaf was performed. About 264,244,210 bp high-quality reads were obtained after strict quality inspection and data cleaning. Compared with the control group, 4,281 genes were differentially expressed in plants treated with drought stress for 7 d (the drought stress group). Compared with the control group, 605 genes showed differential expression in plants subjected to drought stress for 6 d and then watered for 1 d (the rewatering group). Compared with the rewatering group, 5,004 genes were differentially expressed in the drought stress group. In the comparisons between the drought stress and control groups, and between the drought stress and rewatering groups, the pathway that showed the most highly significant enrichment was plant hormone signal transduction. In the comparison between the rewatering and control groups, the pathways that showed the most highly significant enrichment were starch and sucrose metabolism. Eight transcription factors belonging to the AP2/ERF, MYB, NAC, and WRKY families (two transcription factors per family) detected in the leaf transcriptome were associated with the drought stress response. The identified transcription factors provide a basis for further investigation of the response mechanism of kenaf to drought stress. . 2008. Role of the Arabidopsis thaliana NAC transcription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses. X. 2016. Insight into the AP2/ERF transcription factor superfamily in sesame and expression profiling of DREB subfamily under drought stress. BMC Plant Biology 16:171 L. 2006. Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Martinoia E. 2004. Disruption of AtMRP4, a guard cell plasma membrane ABCC-type ABC transporter, leads to deregulation of stomatal opening and increased drought susceptibility.

Transcriptome and proteome analyses of the molecular mechanisms underlying changes in oil storage under drought stress in Brassica napus L.

“…Proteomics, which is the study of the structural and functional features of all the proteins in an organism, is an important means of understanding complex biological mechanisms, including plant responses to abiotic stress tolerance (Deshmukh et al, 2014). Using transcriptomic or proteomics sequencing, many genes associated with drought response were identified in a number of plants, such as Arabidopsis (Cho et al, 2013; Matsui et al, 2008; Meng et al, 2019), rice (Hamzelou et al, 2020; Li et al, 2019), wheat (Fotovat et al, 2017; Kang et al, 2012; Ma et al, 2017; Michaletti et al, 2018), maize (Zeng et al, 2019; Zheng et al, 2020), soybean (Song, Prince, et al, 2016; Wang & Komatsu, 2018), tobacco (Chen et al, 2019; Khan et al, 2019), and others (An et al, 2020; Ghatak et al, 2017; Jangpromma et al, 2010; Ngara & Ndimba, 2013; Raney et al, 2014; Rodziewicz et al, 2019; Singh et al, 2017; Utsumi et al, 2012; Wisniewski et al, 2009; Yang et al, 2017). In rapeseed, numerous candidate genes associated with drought response have also been identified by transcriptomic and proteomics analyses.…”

RNA-Seq Analysis Demonstrates Different Strategies Employed by Tiger Nuts (Cyperus esculentus L.) in Response to Drought Stress

“…In addition, lignin was recognized as having an important role in drought resistance [ 66 ] while the CAD enzyme was used to characterize drought tolerance [ 67 ]. Here, the phenylpropanoid biosynthesis (ko00940), as a stress-resistant pathway [ 68 ], was enriched in the KEGG analysis, while the contents of lignin and CAD activities were significantly changed ( Figure 9 ). In addition, the three pathways have been reported to participate in responding to abiotic stress in Hibiscus cannabinus , Miscanthus and tomato ( Solanum lycopersicum ) [ 69 , 70 , 71 ].…”

The transcription factor HcERF4 confers salt and drought tolerance in kenaf (Hibiscus cannabinus L.)

“…Kenaf has a high yield of biomass and is widely used, including papermaking, ropes, textiles, animal feed, compounding agents, construction and ltration materials, and can also be used for board making and as a source of biomass energy (Li et al 2021b;Tang et al 2019). Based on the characteristics of strong resistance, such as saline (Wei et al 2019), drought (An et al 2020b) and heavy metal (Chen et al 2021b), it is also considered as a good candidate for bioremediation. To uncover the molecular mechanism involved in salt tolerance in kenaf, our previous proteomic study has revealed several key regulatory pathways and identi ed a lot of proteins associated with salt-stress tolerance (Kashif et al 2020b).…”

“…To uncover the molecular mechanism involved in salt tolerance in kenaf, our previous proteomic study has revealed several key regulatory pathways and identi ed a lot of proteins associated with salt-stress tolerance (Kashif et al 2020b). In addition, a lot of transcription factors have been characterized to be-associated with drought stress response by transcriptome analysis (An et al 2020b). However, the salt and drought resistance mechanism of kenaf still needs further study.…”