Transcriptome Analysis and Knockdown of the Juvenile Hormone Esterase Gene Reveal Abnormal Feeding Behavior in the Sugarcane Giant Borer

Noriega, Daniel D.; Arraes, Fabrício Barbosa Monteiro; Antonino, José Dijair; Macedo, Leonardo Lima Pepino; Fonseca, Fernando; Togawa, Roberto Coiti; Grynberg, Priscila; Silva, Maria C. M.; Negrisoli, A. S.; Grossi-de-Sá, Maria Fátima

doi:10.3389/fphys.2020.588450

Cited by 5 publications

(3 citation statements)

References 63 publications

(80 reference statements)

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“…Since the Cry1A family toxins were the most promising, as they presented the lowest LC 50 values (table 1), we performed in silico simulations to predict amino acids that might be involved in the interaction of Cry1A toxins and their putative receptors in T. licus licus . To this end, we employed MD and docking for three putative APN receptors (TlAPN1, TlAPN3, and TlAPN4) whose sequences were previously detected in the T. licus licus transcriptome (Fonseca et al ., 2015; Noriega et al ., 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Later, the same toxin was expressed in Escherichia coli and tested against other lepidopteran pests, conferring its enhanced activity (Tajne et al ., 2013). Two transcriptomes of the sugarcane giant borer have been published, making an important contribution to expanding the genetic information about this insect pest (Fonseca et al ., 2015; Noriega et al ., 2020). Sequencing data revealed APN genes with high expression in T. licus licus midgut tissues (Fonseca et al ., 2015), which could act as potential Cry toxin receptors.…”

Section: Introductionmentioning

confidence: 99%

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In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer

Fonseca

Antonino

Moura

et al. 2023

Bull. Entomol. Res.

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The sugarcane giant borer, Telchin licus licus, is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis (Bt) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin–receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic sugarcane plants resistant to this major occurring insect pest in sugarcane fields.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer

Fonseca

Antonino

Moura

et al. 2023

Bull. Entomol. Res.

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“…Remarkable advances have been made by different groups in developing transgenic and non-transgenic approaches to improve plant resistance to a range of pests [ 17 , 18 , 19 , 20 , 21 ]. Some of the contributions worth highlighting are the discovery and validation of potential target genes to design RNAi molecules to control the CBW [ 22 , 23 , 24 , 25 ], the improvement of the understanding of basic mechanisms of RNAi in insects [ 11 ], and the design of novel approaches to improve RNAi efficacy [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (Anthonomus grandis)

Ribeiro

Vasquez

Macedo

et al. 2022

IJMS

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Cotton is the most important crop for fiber production worldwide. However, the cotton boll weevil (CBW) is an insect pest that causes significant economic losses in infested areas. Current control methods are costly, inefficient, and environmentally hazardous. Herein, we generated transgenic cotton lines expressing double-stranded RNA (dsRNA) molecules to trigger RNA interference-mediated gene silencing in CBW. Thus, we targeted three essential genes coding for chitin synthase 2, vitellogenin, and ecdysis-triggering hormone receptor. The stability of expressed dsRNAs was improved by designing a structured RNA based on a viroid genome architecture. We transformed cotton embryos by inserting a promoter-driven expression cassette that overexpressed the dsRNA into flower buds. The transgenic cotton plants were characterized, and positive PCR transformed events were detected with an average heritability of 80%. Expression of dsRNAs was confirmed in floral buds by RT-qPCR, and the T1 cotton plant generation was challenged with fertilized CBW females. After 30 days, data showed high mortality (around 70%) in oviposited yolks. In adult insects fed on transgenic lines, chitin synthase II and vitellogenin showed reduced expression in larvae and adults, respectively. Developmental delays and abnormalities were also observed in these individuals. Our data remark on the potential of transgenic cotton based on a viroid-structured dsRNA to control CBW.

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Why Biotechnology Needed in Insects?

Rahul

Moteriya

Ghosh

et al. 2023

Learning Materials in Biosciences

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Transcriptome Analysis and Knockdown of the Juvenile Hormone Esterase Gene Reveal Abnormal Feeding Behavior in the Sugarcane Giant Borer

Cited by 5 publications

References 63 publications

In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer

In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer

Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (Anthonomus grandis)

Why Biotechnology Needed in Insects?

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