Transcriptome Analysis and Identification of Genes Associated with Floral Transition and Flower Development in Sugar Apple (Annona squamosa L.)

Abstract

Sugar apple (Annona squamosa L.) is a semi-deciduous subtropical tree that progressively sheds its leaves in the spring. However, little information is available on the mechanism involved in flower developmental pattern. To gain a global perspective on the floral transition and flower development of sugar apple, cDNA libraries were prepared independently from inflorescent meristem and three flowering stages. Illumina sequencing generated 107,197,488 high quality reads that were assembled into 71,948 unigenes, with an average sequence length of 825.40 bp. Among the unigenes, various transcription factor families involved in floral transition and flower development were elucidated. Furthermore, a Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that unigenes exhibiting differential expressions were involved in various phytohormone signal transduction events and circadian rhythms. In addition, 147 unigenes exhibiting sequence similarities to known flowering-related genes from other plants were differentially expressed during flower development. The expression patterns of 20 selected genes were validated using quantitative-PCR. The expression data presented in our study is the most comprehensive dataset available for sugar apple so far and will serve as a resource for investigating the genetics of the flowering process in sugar apple and other Annona species.

A new Annonaceae genus, Wuodendron, provides support for a post–boreotropical origin of the Asian–Neotropical disjunction in the tribe Miliuseae

“…Subpetiolar buds are rare in Annonaceae, and this represents the first such observation in the tribe Miliuseae, although similar buds have previously been recorded in Annona (Soler & Cuevas, 2009;Liu & al., 2016). Wuodendron resembles Meiogyne and Sapranthus in its eucamptodromous leaf venation; this feature is common in the tribe Miliuseae, as well as the closely related tribes Monocarpieae (Mols & Keßler, 2000) and Dendrokingstonieae (Chaowasku & al., 2012b).…”

Novel Cyclic Peptides in Seed of Annona muricata are Ribosomally Synthesized

“…To investigate whether this type of gene is more widespread among the Annonaceae, we downloaded RNA-seq data from the NCBI Sequence Read Archive. These data were generated from RNA isolated from the mature flowers of Annona squamosa (Liu et al 2016) and leaves of Annona muricata (www.onekp.com). We assembled transcriptomes and searched them with tBLASTn using the pamAD transcript as the query sequence.…”

Integrating GWAS , linkage mapping and gene expression analyses reveals the genetic control of growth period traits in rapeseed (Brassica napus L.)

“…A GO and KEGG pathway enrichment analysis indicated that the common DEGs between EV vs. ER and LV vs. LR were enriched for circadian rhythm -plant and salicylic acid biosynthetic process (Fig. S6b), indicating that circadian rhythm and salicylic acid play an important role in the B. napus oral transition, which was also reported in the Annona squamosa and Rosa chinensis [40,41]. We also observed signi cant enrichment for genes involved in the circadian rhythm between LR vs. ER, suggesting that the owering time difference between early-and late-owering lines was primarily due to the expression difference of circadian rhythm genes (Fig.…”