Transcriptome Analyses of Two Citrus Cultivars (Shiranuhi and Huangguogan) in Seedling Etiolation

Xiong, Bo; Ye, Shuang; Qiu, Xia; Liao, Ling; Sun, Guochao; Luo, Jinyu; Dai, Lin; Rong, Yi; Wang, Zhi‐Hui

doi:10.1038/srep46245

Cited by 19 publications

(17 citation statements)

References 69 publications

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“…The germination of seeds from Citrus genotypes was reported to be generally high when seeds are sown immediately after fruit harvest [7][8][9][10][11], although it may differ among species and genotypes [10]. This fact is hypothesized to be due to excessive desiccation, which might cause membrane disruption [7].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, their low and slow germination may represent a main limitation for sprouting purposes, especially for homemade production, where the consumer requires a feasible, short, and cheap way to obtain sprouts. However, the germination of Citrus species is reported to be high when seeds are sown immediately after separation from the fruit pulp [7][8][9][10][11]. Finally, the germination performance and phytochemical content of Citrus sprouts is expected to depend on the genotype.…”

Section: Introductionmentioning

confidence: 99%

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Phenolic Compounds and Antioxidant Activity of Sprouts from Seeds of Citrus Species

et al. 2020

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Seeds from Citrus species represent a relevant by-product of the juice industry and a potential source of bioactive compounds such as phenols and other antioxidants. Sprouting could be an intriguing idea to enhance the content of these compounds, as explored for other fruittree species. In this experiment, the sprouting performance, the concentration of total phenols and phenolic acids, and the antioxidant activity of seeds and sprouts were evaluated for bitter orange (Citrus aurantium L. seedlings), blonde orange (Citrus sinensis (L.) Osbeck cv.Biondocomune), sweet orange (Citrus sinensis (L.) Osbeck seedlings), lemon (Citrus limon (L.) Osbeck cv.Femminello), and mandarin (Citrus reticulata Blanco cv.Tardivo di Ciaculli). The germination was high for all genotypes except for mandarin, but it took 4–8 weeks. Sprouts did not differ among genotypes for size and generally had hard consistency of cotyledons and a bitter taste. The concentrations of total phenols and phenolic acids of seeds and sprouts varied with the genotype, while the antioxidant activity was not statistically different among treatments. Sprouting increased both the concentration of phenolic compounds and antioxidant activity but no correlation was found between them, suggesting that other antioxidants, besides phenols, are present. Given the slow germination and the bitter taste, Citrus sprouts appear unsuitable for homemade production aimed at direct consumption, while they may have perspectives for extraction of food additives, cosmetics, and pharmaceutics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phenolic Compounds and Antioxidant Activity of Sprouts from Seeds of Citrus Species

et al. 2020

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“…We recently analyzed the transcriptome of etiolated citrus seedlings, there were variegated and green seedlings in both Shiranuhi and Huangguogan under the same conditions of seedling germination 23 . Although variegated plants exist widely in nature, the mechanism of citrus leaf variegation and the mechanisms regulating the expression patterns of well-characterized genes involved in photosynthesis and chlorophyll biosynthesis are still unknown.…”

Section: Introductionmentioning

confidence: 99%

Physiological and transcriptome analyses of photosynthesis and chlorophyll metabolism in variegated Citrus (Shiranuhi and Huangguogan) seedlings

Xiong

Qiu

Huang

et al. 2019

Sci Rep

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Citrus species are among the most economically important fruit crops. Physiological characteristics and molecular mechanisms associated with de-etiolation have been partially revealed. However, little is known about the mechanisms controlling the expression and function of genes associated with photosynthesis and chlorophyll biosynthesis in variegated citrus seedlings. The lower biomass, chlorophyll contents, and photosynthetic parameter values recorded for the variegated seedlings suggested that chlorophyll biosynthesis was partially inhibited. Additionally, roots of the variegated seedlings were longer than the roots of green seedlings. We obtained 567.07 million clean reads and 85.05 Gb of RNA-sequencing data, with more than 94.19% of the reads having a quality score of Q30 (sequencing error rate = 0.1%). Furthermore, we detected 4,786 and 7,007 differentially expressed genes (DEGs) between variegated and green Shiranuhi and Huangguogan seedlings. Thirty common pathways were differentially regulated, including pathways related to photosynthesis (GO: 0015979) and the chloroplast (GO: 0009507). Photosynthesis (44 and 63 DEGs), photosynthesis-antenna proteins (14 and 29 DEGs), and flavonoid biosynthesis (16 and 29 DEGs) pathways were the most common KEGG pathways detected in two analyzed libraries. Differences in the expression patterns of PsbQ, PetF, PetB, PsaA, PsaN, PsbP, PsaF, Cluster-2274.8338 (ZIP1), Cluster-2274.38688 (PTC52), and Cluster-2274.78784 might be responsible for the variegation in citrus seedlings. We completed a physiological- and transcriptome-level comparison of the Shiranuhi and Huangguogan cultivars that differ in terms of seedling variegation. We performed mRNA-seq analyses of variegated and green Shiranuhi and Huangguogan seedlings to explore the genes and regulatory pathways involved in the inhibition of chlorophyll biosynthesis and decreases in Chl a and Chl b contents. The candidate genes described herein should be investigated in greater detail to further characterize variegated citrus seedlings.

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“…Huangguogan (Citrus reticulata × Citrus sinensis) is hybrid citrus cultivars, which have been identified as a new cultivated variety in China. In recent years, the plant area of Huangguogan has been expanded rapidly in the southwest of China [2]. Huangguogan was a hybrid variety of Rutaceae Citrus family.…”

Section: Introductionmentioning

confidence: 99%

Effects of Chelated Iron on Roots under Different Mulching Materials on Fruit Quality of Huangguogan

et al. 2020

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In this experiment, Huangguogan in Shimian County was selected as the experimental material, and the effects of different treatments on the fruit quality of Huangguogan were studied by mulching treatments with black film, straw, horticultural cloth and river sand. The results of this study showed that the application of chelated iron to the roots under different mulching materials could significantly improve the fruit quality, and the application of chelated iron to the roots under different mulching materials had different effects on fruit quality. The river sand mulching treatment had the highest single fruit weight, vertical and horizontal diameters, while CK had the lowest one. The four mulching treatments all increased the contents of total soluble solids, soluble sugar and sucrose. The river sand mulching treatment had the highest contents, which were 13.96%, 13.09 and 6.93 g.100 mL-1, respectively. There were significant differences in the titratable acid, vitamins C and sugar-acid ratio between the different mulching treatments, and the river sand mulching treatment had the highest effect. In general, the river sand mulching treatment had the best effect on promoting fruit quality, and plastic film mulching treatment had the worst effect.

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Transcriptome Analyses of Two Citrus Cultivars (Shiranuhi and Huangguogan) in Seedling Etiolation

Cited by 19 publications

References 69 publications

Phenolic Compounds and Antioxidant Activity of Sprouts from Seeds of Citrus Species

Phenolic Compounds and Antioxidant Activity of Sprouts from Seeds of Citrus Species

Physiological and transcriptome analyses of photosynthesis and chlorophyll metabolism in variegated Citrus (Shiranuhi and Huangguogan) seedlings

Effects of Chelated Iron on Roots under Different Mulching Materials on Fruit Quality of Huangguogan

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