Transcriptome analyses of the ginseng root rot pathogens Cylindrocarpon destructans and Fusarium solani to identify radicicol resistance mechanisms

Li, Taiying; Kim, Jin-Hyun; Jung, Boknam; Ji, Sungyeon; Seo, Mun Won; Han, You Kyoung; Lee, Sung Woo; Bae, Yeoung Seuk; Choi, Hong-Gyu; Lee, Seung-Ho; Lee, Jungkwan

doi:10.1016/j.jgr.2018.11.005

Cited by 16 publications

(8 citation statements)

References 41 publications

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“…A synergistic action of JA and ethylene (ET) signaling pathways was suggested to positively regulate the defense response in P. notoginseng [14]. On the other hand, transcriptomes of F. solani have been studied in the F. solani-ginseng pathosystem and largely explored pathogen genes related to chitin synthase and cell wall integrity genes [15]. The non-host disease resistance response of pea (Pisum sativum L.) to F. solani was explored, and pea endocarp tissues were described have a 6-h time window for resistance [12].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Chen¹,

Wu²,

He³

et al. 2020

Genes

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Common bean (Phaseolus vulgaris L.) is a major legume and is frequently attacked by fungal pathogens, including Fusarium solani f. sp. phaseoli (FSP), which cause Fusarium root rot. FSP substantially reduces common bean yields across the world, including China, but little is known about how common bean plants defend themselves against this fungal pathogen. In the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and metabolomic processes in common bean infected with FSP. There were 29,722 differentially regulated genes and 300 differentially regulated metabolites between control and infected plants. The combined omics approach revealed that FSP is perceived by PAMP-triggered immunity and effector-triggered immunity. Infected seedlings showed that common bean responded by cell wall modification, ROS generation, and a synergistic hormone-driven defense response. Further analysis showed that FSP induced energy metabolism, nitrogen mobilization, accumulation of sugars, and arginine and proline metabolism. Importantly, metabolic pathways were most significantly enriched, which resulted in increased levels of metabolites that were involved in the plant defense response. A correspondence between the transcript pattern and metabolite profile was observed in the discussed pathways. The combined omics approach enhances our understanding of the less explored pathosystem and will provide clues for the development of common bean cultivars’ resistant to FSP.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Chen¹,

Wu²,

He³

et al. 2020

Genes

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“…GST is a detoxifying enzyme ( Dasari et al, 2018 ) that was significantly downregulated in the present study. MFS transporters are more selective than ABC transporters in the efflux of toxin compounds ( Lee et al, 2011 ) previous research has shown that the deletion of MFS and ABC transporters inhibits FP growth ( Li et al, 2020 ). In the present study, the MFS transporter (HYE68_002746) was significantly downregulated, which inhibited the ability of Fp to extract toxic compounds, thereby reducing pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic effects of Talaromyces muroii TM28 against Fusarium crown rot of wheat caused by Fusarium pseudograminearum

Yang,

Cui,

Wei

et al. 2024

Front. Microbiol.

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Fusarium crown rot (FCR) caused by Fusarium pseudograminearum is a serious threat to wheat production worldwide. This study aimed to assess the effects of Talaromyces muroii strain TM28 isolated from root of Panax quinquefolius against F. pseudograminearum. The strain of TM28 inhibited mycelial growth of F. pseudograminearum by 87.8% at 72 h, its cell free fermentation filtrate had a strong antagonistic effect on mycelial growth and conidial germination of F. pseudograminearum by destroying the integrity of the cell membrane. In the greenhouse, TM28 significantly increased wheat fresh weight and height in the presence of pathogen Fp, it enhanced the antioxidant defense activity and ameliorated the negative effects of F. pseudograminearum, including disease severity and pathogen abundance in the rhizosphere soil, root and stem base of wheat. RNA-seq of F. pseudograminearum under TM28 antagonistic revealed 2,823 differentially expressed genes (DEGs). Most DEGs related to cell wall and cell membrane synthesis were significantly downregulated, the culture filtrate of TM28 affected the pathways of fatty acid synthesis, steroid synthesis, glycolysis, and the citrate acid cycle. T. muroii TM28 appears to have significant potential in controlling wheat Fusarium crown rot caused by F. pseudograminearum.

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“…For thousands of years, ginseng has been widely cultivated for its medicinal value ( Xiao et al, 2016 ). Ginseng root rot was caused by Fusarium spp., Fusarium solani was one of the most important pathogens that can reduce the yield and quality of ginseng, thereby seriously constraining the economic development of ginseng ( Li et al, 2020 ). Currently, the disease is controlled by applying synthetic fungicides and chemical products.…”

Section: Introductionmentioning

confidence: 99%

Bioassay-guided isolation of three new alkaloids from Suillus bovinus and preliminary mechanism against ginseng root rot

Xiong,

Yang,

Yao

et al. 2024

Front. Microbiol.

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In order to control the occurrence of ginseng root rot caused by Fusarium solani (Mart.) Sacc., the antifungal compounds of the mushroom Suillus bovinus were investigated. And three new alkaloids (1–3), named bovinalkaloid A–C, along with one known analog (4), were isolated and identified by bioassay-guided isolation and spectroscopic analyses. Compound 1 strongly inhibited the mycelial growth and spore germination of F. solani with minimum inhibitory concentration of 2.08 mM. Increases in electrical conductivity, nucleic acid, and protein contents, and decreases in lipid content showed that the membrane permeability and integrity were damaged by compound 1. Compound 1 also increased the contents of malondialdehyde and hydrogen peroxide and the activities of antioxidant enzymes, indicating that lipid peroxidation had taken place in F. solani. Compound 1 may serve as a natural alternative to synthetic fungicides for the control of ginseng root rot.

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Transcriptome analyses of the ginseng root rot pathogens Cylindrocarpon destructans and Fusarium solani to identify radicicol resistance mechanisms

Cited by 16 publications

References 41 publications

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Antagonistic effects of Talaromyces muroii TM28 against Fusarium crown rot of wheat caused by Fusarium pseudograminearum

Bioassay-guided isolation of three new alkaloids from Suillus bovinus and preliminary mechanism against ginseng root rot

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